Gene description for FRAT1
Gene name frequently rearranged in advanced T-cell lymphomas 1
Gene symbol FRAT1
Other names/aliases -
Species Homo sapiens
 Database cross references - FRAT1
ExoCarta ExoCarta_10023
Vesiclepedia VP_10023
Entrez Gene 10023
HGNC 3944
MIM 602503
UniProt Q92837  
 FRAT1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 25330373    
 Gene ontology annotations for FRAT1
Molecular Function
    protein binding GO:0005515 IPI
    molecular function inhibitor activity GO:0140678 IDA
Biological Process
    regulation of protein export from nucleus GO:0046825 IDA
    canonical Wnt signaling pathway GO:0060070 IDA
    canonical Wnt signaling pathway GO:0060070 IGI
    positive regulation of canonical Wnt signaling pathway GO:0090263 IBA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IGI
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified FRAT1 in sEVs
1
Experiment ID 223
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25330373    
Organism Homo sapiens
Experiment description Deep sequencing of RNA from three different extracellular vesicle (EV) subtypes released from the human LIM1863 colon cancer cell line uncovers distinct miRNA-enrichment signatures
Authors "Ji H, Chen M, Greening DW, He W, Rai A, Zhang W, Simpson RJ"
Journal name PLoS One
Publication year 2014
Sample Colorectal cancer cells
Sample name LIM1863
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Immunobeads (EpCAM)
Immunobeads (A33)
Flotation density -
Molecules identified in the study miRNA
Protein
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for FRAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GPRASP2  
Two-hybrid Homo sapiens
2 KRT18 3875
Two-hybrid Homo sapiens
3 GSK3A 2931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LMAN2 10960
Two-hybrid Homo sapiens
5 KRAS 3845
Affinity Capture-MS Homo sapiens
6 LAMC3  
Two-hybrid Homo sapiens
7 DVL1 1855
Affinity Capture-Western Homo sapiens
8 GRN 2896
Two-hybrid Homo sapiens
9 PSMA3 5684
Two-hybrid Homo sapiens
10 PLSCR1 5359
Two-hybrid Homo sapiens
11 LTBP4 8425
Two-hybrid Homo sapiens
12 TDRD7 23424
Two-hybrid Homo sapiens
13 MAP3K1 4214
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
14 GSK3B 2932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 RABEP2 79874
Two-hybrid Homo sapiens
16 PIAS1 8554
Affinity Capture-MS Homo sapiens
17 KCNK5  
Two-hybrid Homo sapiens
View the network image/svg+xml



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