Gene description for PNPLA6
Gene name patatin-like phospholipase domain containing 6
Gene symbol PNPLA6
Other names/aliases BNHS
LNMS
NTE
NTEMND
OMCS
SPG39
iPLA2delta
sws
Species Homo sapiens
 Database cross references - PNPLA6
ExoCarta ExoCarta_10908
Vesiclepedia VP_10908
Entrez Gene 10908
HGNC 16268
MIM 603197
UniProt Q8IY17  
 PNPLA6 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 25452312    
 Gene ontology annotations for PNPLA6
Molecular Function
    lysophospholipase activity GO:0004622 EXP
    lysophospholipase activity GO:0004622 IBA
    lysophospholipase activity GO:0004622 ISS
Biological Process
    phosphatidylcholine metabolic process GO:0046470 IEA
    glycerophospholipid catabolic process GO:0046475 TAS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
 Experiment description of studies that identified PNPLA6 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 238
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25452312    
Organism Homo sapiens
Experiment description N-linked (N-) glycoproteomics of urimary exosomes
Authors "Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R."
Journal name Mol Cell Proteomics
Publication year 2014
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PNPLA6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRP3  
Affinity Capture-MS Homo sapiens
2 SDC2 6383
Affinity Capture-MS Homo sapiens
3 NKAIN1  
Affinity Capture-MS Homo sapiens
4 HFE  
Affinity Capture-MS Homo sapiens
5 GRK5 2869
Affinity Capture-MS Homo sapiens
6 KCNS3 3790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 Rab5c 19345
Affinity Capture-MS Mus musculus
8 ZNRF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RAMP2  
Affinity Capture-MS Homo sapiens
10 FAF2 23197
Affinity Capture-MS Homo sapiens
11 BAX 581
Affinity Capture-MS Homo sapiens
12 TTYH1  
Affinity Capture-MS Homo sapiens
13 CUL3 8452
Affinity Capture-MS Homo sapiens
14 CANX 821
Affinity Capture-MS Homo sapiens
15 CXCR4 7852
Affinity Capture-MS Homo sapiens
16 EGLN3  
Affinity Capture-MS Homo sapiens
17 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 HCST  
Affinity Capture-MS Homo sapiens
19 PNPLA7  
Affinity Capture-MS Homo sapiens
20 CDH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PDCD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PCDHGB5 56101
Affinity Capture-MS Homo sapiens
24 NEUROG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TSPAN17 26262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 STIM1 6786
Affinity Capture-MS Homo sapiens
27 IL17RA 23765
Affinity Capture-MS Homo sapiens
28 EMD 2010
Affinity Capture-MS Homo sapiens
29 CTDP1 9150
Affinity Capture-MS Homo sapiens
30 PCDHB6  
Affinity Capture-MS Homo sapiens
31 PARK2  
Affinity Capture-MS Homo sapiens
32 Ktn1  
Affinity Capture-MS Mus musculus
33 FAXC  
Affinity Capture-MS Homo sapiens
34 Ppp6c 67857
Affinity Capture-MS Mus musculus
35 GPRASP2  
Affinity Capture-MS Homo sapiens
36 IFNLR1  
Affinity Capture-MS Homo sapiens
37 NXF1 10482
Affinity Capture-RNA Homo sapiens
38 PCDHB11  
Affinity Capture-MS Homo sapiens
39 EVA1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 PGRMC2 10424
Affinity Capture-MS Homo sapiens
41 TEX264 51368
Affinity Capture-MS Homo sapiens
42 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
43 Srp72  
Affinity Capture-MS Mus musculus
44 C3orf52  
Affinity Capture-MS Homo sapiens
45 BRD1 23774
Affinity Capture-MS Homo sapiens
46 HLA-DPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PCDHGC4  
Affinity Capture-MS Homo sapiens
48 CTLA4  
Affinity Capture-MS Homo sapiens
49 NTRK1 4914
Affinity Capture-MS Homo sapiens
50 LRRTM1 347730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PLD3 23646
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
52 EVA1B 55194
Affinity Capture-MS Homo sapiens
53 MOV10 4343
Affinity Capture-RNA Homo sapiens
54 FBXW7  
Affinity Capture-MS Homo sapiens
55 PLXDC2 84898
Affinity Capture-MS Homo sapiens
56 PCDHB16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 GDI1 2664
Affinity Capture-MS Homo sapiens
58 DNAJC1 64215
Proximity Label-MS Homo sapiens
59 VIPR1  
Affinity Capture-MS Homo sapiens
60 ILK 3611
Affinity Capture-MS Homo sapiens
61 KRAS 3845
Negative Genetic Homo sapiens
62 NUP35 129401
Proximity Label-MS Homo sapiens
63 PCDHB3  
Affinity Capture-MS Homo sapiens
64 FSCN1 6624
Affinity Capture-MS Homo sapiens
65 EFNB2 1948
Affinity Capture-MS Homo sapiens
66 MRAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 PDE3A  
Affinity Capture-MS Homo sapiens
68 PCDHGC5  
Affinity Capture-MS Homo sapiens
69 CLRN2  
Affinity Capture-MS Homo sapiens
70 CHRND  
Affinity Capture-MS Homo sapiens
71 MMGT1 93380
Affinity Capture-MS Homo sapiens
72 HIF1A 3091
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PNPLA6 is involved
PathwayEvidenceSource
Glycerophospholipid catabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Phospholipid metabolism TAS Reactome
PI Metabolism TAS Reactome





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