Gene description for Rps6ka3
Gene name ribosomal protein S6 kinase polypeptide 3
Gene symbol Rps6ka3
Other names/aliases C130006E23
MAPKAPK-1b
MPK-9
Rsk2
S6K-alpha3
p90RSK3
pp90RSK2
Species Mus musculus
 Database cross references - Rps6ka3
ExoCarta ExoCarta_110651
Vesiclepedia VP_110651
Entrez Gene 110651
UniProt P18654  
 Rps6ka3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rps6ka3
Molecular Function
    magnesium ion binding GO:0000287 IEA
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IBA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 IPI
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 IEA
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 ISO
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    toll-like receptor signaling pathway GO:0002224 IMP
    chromatin remodeling GO:0006338 IEA
    response to lipopolysaccharide GO:0032496 IMP
    intracellular signal transduction GO:0035556 IEA
    TORC1 signaling GO:0038202 IBA
    negative regulation of apoptotic process GO:0043066 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    negative regulation of cysteine-type endopeptidase activity GO:2000117 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Rps6ka3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rps6ka3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Affinity Capture-Western Homo sapiens
2 Tmem173  
Proximity Label-MS Mus musculus
3 Grin2b  
Affinity Capture-Western Mus musculus
4 Foxp3  
Affinity Capture-MS Mus musculus
5 Crebbp  
Affinity Capture-Western Mus musculus
6 Mapk1 26413
Biochemical Activity Mus musculus
7 MAPK3 5595
Affinity Capture-Western Homo sapiens
8 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
9 ABCG1  
Affinity Capture-MS Homo sapiens
10 Mapk3 26417
Biochemical Activity Mus musculus
11 Grin1  
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Rps6ka3 is involved
PathwayEvidenceSource
Activation of NMDA receptors and postsynaptic events IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Cellular Senescence IEA Reactome
CREB phosphorylation IEA Reactome
CREB1 phosphorylation through NMDA receptor-mediated activation of RAS signaling IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
ERK/MAPK targets IEA Reactome
G alpha (q) signalling events IEA Reactome
Gastrin-CREB signalling pathway via PKC and MAPK IEA Reactome
GPCR downstream signalling IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interleukin-17 signaling IEA Reactome
MAP kinase activation IEA Reactome
MAPK targets/ Nuclear events mediated by MAP kinases IEA Reactome
MyD88 cascade initiated on plasma membrane IEA Reactome
MyD88 dependent cascade initiated on endosome IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Nuclear Events (kinase and transcription factor activation) IEA Reactome
Post NMDA receptor activation events IEA Reactome
RSK activation IEA Reactome
Senescence-Associated Secretory Phenotype (SASP) IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Toll Like Receptor 10 (TLR10) Cascade IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade IEA Reactome
Toll Like Receptor 3 (TLR3) Cascade IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll Like Receptor 5 (TLR5) Cascade IEA Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade IEA Reactome
Toll Like Receptor 9 (TLR9) Cascade IEA Reactome
Toll Like Receptor TLR1:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR6:TLR2 Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation IEA Reactome
Transmission across Chemical Synapses IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome





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