Gene description for Mapk1
Gene name mitogen-activated protein kinase 1
Gene symbol Mapk1
Other names/aliases 9030612K14Rik
AA407128
AU018647
C78273
ERK
Erk2
MAPK2
PRKM2
Prkm1
p41mapk
p42mapk
Species Mus musculus
 Database cross references - Mapk1
ExoCarta ExoCarta_26413
Vesiclepedia VP_26413
Entrez Gene 26413
UniProt P63085  
 Mapk1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mapk1
Molecular Function
    phosphotyrosine residue binding GO:0001784 IMP
    double-stranded DNA binding GO:0003690 ISO
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 TAS
    JUN kinase activity GO:0004705 IEA
    MAP kinase activity GO:0004707 IDA
    MAP kinase activity GO:0004707 IMP
    MAP kinase activity GO:0004707 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    RNA polymerase II CTD heptapeptide repeat kinase activity GO:0008353 IDA
    kinase activity GO:0016301 IDA
    protein kinase binding GO:0019901 ISO
    phosphatase binding GO:0019902 IPI
    phosphatase binding GO:0019902 ISO
    mitogen-activated protein kinase kinase kinase binding GO:0031435 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    protein serine kinase activity GO:0106310 IEA
Biological Process
    MAPK cascade GO:0000165 IDA
    MAPK cascade GO:0000165 IGI
    MAPK cascade GO:0000165 ISO
    DNA-templated transcription GO:0006351 NAS
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 IMP
    protein phosphorylation GO:0006468 ISO
    apoptotic process GO:0006915 IEA
    DNA damage response GO:0006974 IDA
    cell surface receptor signaling pathway GO:0007166 IBA
    JNK cascade GO:0007254 IEA
    heart development GO:0007507 IMP
    insulin receptor signaling pathway GO:0008286 IDA
    response to toxic substance GO:0009636 ISO
    animal organ morphogenesis GO:0009887 IDA
    positive regulation of macrophage chemotaxis GO:0010759 IEA
    positive regulation of macrophage chemotaxis GO:0010759 ISO
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 ISO
    neural crest cell development GO:0014032 IGI
    Schwann cell development GO:0014044 IMP
    diadenosine tetraphosphate biosynthetic process GO:0015966 ISO
    peptidyl-serine phosphorylation GO:0018105 IDA
    peptidyl-serine phosphorylation GO:0018105 IDA
    peptidyl-serine phosphorylation GO:0018105 IMP
    peptidyl-serine phosphorylation GO:0018105 ISO
    peptidyl-serine phosphorylation GO:0018105 ISO
    peptidyl-threonine phosphorylation GO:0018107 IDA
    sensory perception of pain GO:0019233 ISO
    cytosine metabolic process GO:0019858 IDA
    regulation of ossification GO:0030278 IGI
    androgen receptor signaling pathway GO:0030521 IEA
    androgen receptor signaling pathway GO:0030521 ISO
    regulation of cellular pH GO:0030641 IMP
    thyroid gland development GO:0030878 IGI
    regulation of protein stability GO:0031647 ISS
    lipopolysaccharide-mediated signaling pathway GO:0031663 IDA
    positive regulation of telomere maintenance GO:0032206 IEA
    positive regulation of telomere maintenance via telomerase GO:0032212 ISO
    response to lipopolysaccharide GO:0032496 IDA
    regulation of stress-activated MAPK cascade GO:0032872 TAS
    mammary gland epithelial cell proliferation GO:0033598 IDA
    cellular response to amino acid starvation GO:0034198 IEA
    cellular response to amino acid starvation GO:0034198 ISO
    cellular response to reactive oxygen species GO:0034614 ISO
    response to nicotine GO:0035094 IGI
    response to nicotine GO:0035094 ISO
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 ISO
    ERBB signaling pathway GO:0038127 ISO
    ERBB2-ERBB3 signaling pathway GO:0038133 IDA
    positive regulation of protein import into nucleus GO:0042307 ISO
    outer ear morphogenesis GO:0042473 IGI
    myelination GO:0042552 IMP
    response to exogenous dsRNA GO:0043330 IDA
    steroid hormone receptor signaling pathway GO:0043401 ISO
    response to estrogen GO:0043627 ISO
    positive regulation of cholesterol biosynthetic process GO:0045542 IEA
    negative regulation of cell differentiation GO:0045596 IGI
    positive regulation of translation GO:0045727 ISO
    positive regulation of DNA-templated transcription GO:0045893 ISO
    decidualization GO:0046697 ISO
    insulin-like growth factor receptor signaling pathway GO:0048009 IDA
    thymus development GO:0048538 IGI
    progesterone receptor signaling pathway GO:0050847 IEA
    progesterone receptor signaling pathway GO:0050847 ISO
    T cell receptor signaling pathway GO:0050852 IDA
    B cell receptor signaling pathway GO:0050853 IDA
    regulation of DNA-binding transcription factor activity GO:0051090 NAS
    stress-activated MAPK cascade GO:0051403 IEA
    stress-activated MAPK cascade GO:0051403 ISO
    regulation of cytoskeleton organization GO:0051493 TAS
    Bergmann glial cell differentiation GO:0060020 IGI
    positive regulation of cardiac muscle cell proliferation GO:0060045 ISO
    long-term synaptic potentiation GO:0060291 IGI
    face development GO:0060324 IGI
    face development GO:0060324 IMP
    lung morphogenesis GO:0060425 IGI
    trachea formation GO:0060440 IGI
    labyrinthine layer blood vessel development GO:0060716 IMP
    cardiac neural crest cell development involved in heart development GO:0061308 IGI
    ERK1 and ERK2 cascade GO:0070371 IGI
    ERK1 and ERK2 cascade GO:0070371 ISO
    response to epidermal growth factor GO:0070849 ISO
    response to epidermal growth factor GO:0070849 ISS
    cellular response to cadmium ion GO:0071276 ISO
    cellular response to tumor necrosis factor GO:0071356 IGI
    caveolin-mediated endocytosis GO:0072584 TAS
    regulation of Golgi inheritance GO:0090170 TAS
    cellular response to toxic substance GO:0097237 ISO
    positive regulation of macrophage proliferation GO:0120041 IEA
    positive regulation of macrophage proliferation GO:0120041 ISO
    positive regulation of telomere capping GO:1904355 ISO
    regulation of early endosome to late endosome transport GO:2000641 TAS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 TAS
    early endosome GO:0005769 TAS
    late endosome GO:0005770 TAS
    Golgi apparatus GO:0005794 TAS
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 TAS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    caveola GO:0005901 ISO
    caveola GO:0005901 ISS
    caveola GO:0005901 TAS
    focal adhesion GO:0005925 TAS
    postsynaptic density GO:0014069 ISO
    axon GO:0030424 ISO
    pseudopodium GO:0031143 IDA
    dendrite cytoplasm GO:0032839 ISO
    protein-containing complex GO:0032991 ISO
    perikaryon GO:0043204 ISO
    mitotic spindle GO:0072686 ISS
 Experiment description of studies that identified Mapk1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mapk1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vrk3  
Affinity Capture-Western Mus musculus
2 KLF11  
Biochemical Activity Homo sapiens
3 Ptpn5  
Reconstituted Complex Mus musculus
4 Tnik  
Affinity Capture-MS Mus musculus
5 Capn2 12334
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
6 Pde4dip  
Affinity Capture-MS Mus musculus
7 Pea15b  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Co-localization Mus musculus
8 Mbp 17196
Biochemical Activity Mus musculus
9 Dusp3  
Affinity Capture-Western Mus musculus
10 TP53 7157
Biochemical Activity Homo sapiens
11 Rps6ka3 110651
Biochemical Activity Mus musculus
12 Dusp2  
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
13 Nras 18176
Synthetic Lethality Mus musculus
14 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
15 Syne2  
Co-fractionation Mus musculus
16 Rps6ka2 20112
Co-fractionation Mus musculus
17 Syngap1  
Affinity Capture-MS Mus musculus
18 Mapt  
Affinity Capture-MS Mus musculus
19 Fmr1  
Affinity Capture-MS Mus musculus
20 Kctd13  
Affinity Capture-MS Mus musculus
21 Mapk3 26417
Phenotypic Suppression Mus musculus
22 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
23 Grin2b  
Affinity Capture-Western Mus musculus
24 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
25 Cttn  
Affinity Capture-Western Mus musculus
26 Elk1  
Biochemical Activity Mus musculus
Biochemical Activity Mus musculus
Biochemical Activity Mus musculus
27 Eif4ebp1  
Biochemical Activity Mus musculus
28 Ntn1  
Co-fractionation Mus musculus
29 Stat1 20846
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
30 Mapk6  
Phenotypic Suppression Mus musculus
31 Itgb5 16419
Affinity Capture-Western Mus musculus
Protein-peptide Mus musculus
32 Blnk  
Affinity Capture-Western Mus musculus
33 Map2k1 26395
Biochemical Activity Mus musculus
Biochemical Activity Mus musculus
34 Pcyt1a  
Affinity Capture-Western Mus musculus
35 PTPN5  
Reconstituted Complex Homo sapiens
36 Eif4e3  
Co-fractionation Mus musculus
37 Pea15a  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
38 Agap2  
Affinity Capture-MS Mus musculus
39 Nanog  
Two-hybrid Mus musculus
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
40 Grin1  
Affinity Capture-Western Mus musculus
41 Eif4ebp2  
Biochemical Activity Mus musculus
42 Mapk8ip3  
Affinity Capture-Western Mus musculus
43 Top2a 21973
Affinity Capture-Western Mus musculus
44 Fancd2  
Affinity Capture-MS Mus musculus
45 Sqstm1  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
46 Eed  
Affinity Capture-MS Mus musculus
47 Atxn1  
Affinity Capture-MS Mus musculus
48 Tia1  
Affinity Capture-MS Mus musculus
49 Ksr1  
Affinity Capture-Western Mus musculus
50 Pxn  
Reconstituted Complex Mus musculus
View the network image/svg+xml
 Pathways in which Mapk1 is involved
PathwayEvidenceSource
Activation of the AP-1 family of transcription factors IEA Reactome
Apoptosis IEA Reactome
Apoptotic factor-mediated response IEA Reactome
Axon guidance IEA Reactome
Ca-dependent events IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Cellular Senescence IEA Reactome
Cytochrome c-mediated apoptotic response IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Developmental Biology IEA Reactome
Downregulation of SMAD2/3:SMAD4 transcriptional activity IEA Reactome
ERK/MAPK targets IEA Reactome
ERKs are inactivated IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-dependent nuclear events downstream of ESR-membrane signaling IEA Reactome
Estrogen-stimulated signaling through PRKCZ IEA Reactome
Extra-nuclear estrogen signaling IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated MAPK activation IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
Formation of apoptosome IEA Reactome
Frs2-mediated activation IEA Reactome
G alpha (i) signalling events IEA Reactome
G alpha (q) signalling events IEA Reactome
G-protein mediated events IEA Reactome
Gastrin-CREB signalling pathway via PKC and MAPK IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
Golgi Cisternae Pericentriolar Stack Reorganization IEA Reactome
GPCR downstream signalling IEA Reactome
Growth hormone receptor signaling IEA Reactome
Hemostasis IEA Reactome
IFNG signaling activates MAPKs IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Insulin receptor signalling cascade IEA Reactome
Interferon gamma signaling IEA Reactome
Interferon Signaling IEA Reactome
Interleukin-17 signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
Intrinsic Pathway for Apoptosis IEA Reactome
L1CAM interactions IEA Reactome
M Phase IEA Reactome
MAP kinase activation IEA Reactome
MAP2K and MAPK activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK targets/ Nuclear events mediated by MAP kinases IEA Reactome
MAPK1 (ERK2) activation IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
MITF-M-regulated melanocyte development IEA Reactome
Mitotic Prophase IEA Reactome
MyD88 cascade initiated on plasma membrane IEA Reactome
MyD88 dependent cascade initiated on endosome IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane IEA Reactome
NCAM signaling for neurite out-growth IEA Reactome
Negative feedback regulation of MAPK pathway IEA Reactome
Negative regulation of FGFR1 signaling IEA Reactome
Negative regulation of FGFR2 signaling IEA Reactome
Negative regulation of FGFR3 signaling IEA Reactome
Negative regulation of FGFR4 signaling IEA Reactome
Negative regulation of MAPK pathway IEA Reactome
Negative regulation of the PI3K/AKT network IEA Reactome
Nervous system development IEA Reactome
Neutrophil degranulation IEA Reactome
Nuclear Events (kinase and transcription factor activation) IEA Reactome
Oncogene Induced Senescence IEA Reactome
Opioid Signalling IEA Reactome
Oxidative Stress Induced Senescence IEA Reactome
phospho-PLA2 pathway IEA Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
PLC beta mediated events IEA Reactome
Programmed Cell Death IEA Reactome
Prolonged ERK activation events IEA Reactome
RAF-independent MAPK1/3 activation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Recycling pathway of L1 IEA Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
Regulation of HSF1-mediated heat shock response IEA Reactome
Regulation of the apoptosome activity IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate NADPH Oxidases IEA Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Senescence-Associated Secretory Phenotype (SASP) IEA Reactome
Signal attenuation IEA Reactome
Signal Transduction IEA Reactome
Signal transduction by L1 IEA Reactome
Signaling by Activin IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Insulin receptor IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by NODAL IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by TGF-beta Receptor Complex IEA Reactome
Signaling by TGFB family members IEA Reactome
Signalling to ERKs IEA Reactome
SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription IEA Reactome
Spry regulation of FGF signaling IEA Reactome
Thrombin signalling through proteinase activated receptors (PARs) IEA Reactome
Toll Like Receptor 10 (TLR10) Cascade IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade IEA Reactome
Toll Like Receptor 3 (TLR3) Cascade IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll Like Receptor 5 (TLR5) Cascade IEA Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade IEA Reactome
Toll Like Receptor 9 (TLR9) Cascade IEA Reactome
Toll Like Receptor TLR1:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR6:TLR2 Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation IEA Reactome
Transcriptional activity of SMAD2/SMAD3:SMAD4 heterotrimer IEA Reactome
Transcriptional and post-translational regulation of MITF-M expression and activity IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome





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