Gene description for Mapk3
Gene name mitogen-activated protein kinase 3
Gene symbol Mapk3
Other names/aliases Erk-1
Erk1
Ert2
Esrk1
Mnk1
Mtap2k
Prkm3
p44
p44erk1
p44mapk
Species Mus musculus
 Database cross references - Mapk3
ExoCarta ExoCarta_26417
Vesiclepedia VP_26417
Entrez Gene 26417
UniProt Q63844  
 Mapk3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mapk3
Molecular Function
    phosphotyrosine residue binding GO:0001784 IMP
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 TAS
    JUN kinase activity GO:0004705 IEA
    MAP kinase activity GO:0004707 IDA
    MAP kinase activity GO:0004707 IMP
    MAP kinase activity GO:0004707 ISO
    MAP kinase activity GO:0004707 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    phosphatase binding GO:0019902 IEA
    phosphatase binding GO:0019902 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    scaffold protein binding GO:0097110 ISO
    protein serine kinase activity GO:0106310 IEA
    DNA-binding transcription factor binding GO:0140297 IEA
    DNA-binding transcription factor binding GO:0140297 ISO
Biological Process
    MAPK cascade GO:0000165 IGI
    MAPK cascade GO:0000165 ISO
    DNA-templated transcription GO:0006351 IMP
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 ISO
    apoptotic process GO:0006915 IEA
    DNA damage response GO:0006974 IDA
    signal transduction GO:0007165 TAS
    cell surface receptor signaling pathway GO:0007166 IBA
    JNK cascade GO:0007254 IEA
    insulin receptor signaling pathway GO:0008286 IDA
    response to toxic substance GO:0009636 ISO
    animal organ morphogenesis GO:0009887 IDA
    positive regulation of macrophage chemotaxis GO:0010759 IEA
    positive regulation of macrophage chemotaxis GO:0010759 ISO
    neural crest cell development GO:0014032 IGI
    Schwann cell development GO:0014044 IMP
    phosphorylation GO:0016310 ISO
    phosphorylation GO:0016310 TAS
    sensory perception of pain GO:0019233 IMP
    regulation of ossification GO:0030278 IGI
    BMP signaling pathway GO:0030509 IEA
    BMP signaling pathway GO:0030509 ISO
    regulation of cellular pH GO:0030641 IMP
    thyroid gland development GO:0030878 IGI
    positive regulation of cyclase activity GO:0031281 ISO
    lipopolysaccharide-mediated signaling pathway GO:0031663 IDA
    positive regulation of telomere maintenance GO:0032206 IEA
    positive regulation of telomere maintenance via telomerase GO:0032212 ISO
    response to lipopolysaccharide GO:0032496 IDA
    regulation of stress-activated MAPK cascade GO:0032872 TAS
    cellular response to amino acid starvation GO:0034198 IEA
    cellular response to amino acid starvation GO:0034198 ISO
    cellular response to reactive oxygen species GO:0034614 ISO
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 ISO
    peptidyl-tyrosine autophosphorylation GO:0038083 ISO
    ERBB2-ERBB3 signaling pathway GO:0038133 IDA
    outer ear morphogenesis GO:0042473 IGI
    myelination GO:0042552 IMP
    signal transduction in response to DNA damage GO:0042770 IEA
    signal transduction in response to DNA damage GO:0042770 ISO
    response to exogenous dsRNA GO:0043330 IDA
    positive regulation of translation GO:0045727 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    decidualization GO:0046697 ISO
    insulin-like growth factor receptor signaling pathway GO:0048009 IDA
    insulin-like growth factor receptor signaling pathway GO:0048009 ISO
    thymus development GO:0048538 IGI
    modulation of chemical synaptic transmission GO:0050804 IDA
    modulation of chemical synaptic transmission GO:0050804 IMP
    regulation of DNA-binding transcription factor activity GO:0051090 IMP
    cartilage development GO:0051216 IDA
    stress-activated MAPK cascade GO:0051403 IEA
    stress-activated MAPK cascade GO:0051403 ISO
    regulation of cytoskeleton organization GO:0051493 TAS
    Bergmann glial cell differentiation GO:0060020 IGI
    face development GO:0060324 IGI
    lung morphogenesis GO:0060425 IGI
    trachea formation GO:0060440 IGI
    cardiac neural crest cell development involved in heart development GO:0061308 IGI
    protein-containing complex assembly GO:0065003 ISO
    ERK1 and ERK2 cascade GO:0070371 IGI
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    interleukin-1-mediated signaling pathway GO:0070498 IEA
    interleukin-1-mediated signaling pathway GO:0070498 ISO
    response to epidermal growth factor GO:0070849 ISO
    response to epidermal growth factor GO:0070849 ISS
    cellular response to mechanical stimulus GO:0071260 IEA
    cellular response to cadmium ion GO:0071276 ISO
    cellular response to tumor necrosis factor GO:0071356 IGI
    caveolin-mediated endocytosis GO:0072584 TAS
    regulation of Golgi inheritance GO:0090170 TAS
    cellular response to toxic substance GO:0097237 ISO
    xenophagy GO:0098792 IGI
    positive regulation of macrophage proliferation GO:0120041 IEA
    positive regulation of macrophage proliferation GO:0120041 ISO
    negative regulation of TORC1 signaling GO:1904262 IEA
    negative regulation of TORC1 signaling GO:1904262 ISO
    positive regulation of telomere capping GO:1904355 ISO
    positive regulation of xenophagy GO:1904417 IGI
    regulation of early endosome to late endosome transport GO:2000641 TAS
    negative regulation of apolipoprotein binding GO:2000657 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 TAS
    nuclear envelope GO:0005635 IEA
    nuclear envelope GO:0005635 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 TAS
    early endosome GO:0005769 TAS
    late endosome GO:0005770 TAS
    Golgi apparatus GO:0005794 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 TAS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    caveola GO:0005901 ISO
    caveola GO:0005901 ISS
    caveola GO:0005901 TAS
    focal adhesion GO:0005925 TAS
    pseudopodium GO:0031143 IDA
    protein-containing complex GO:0032991 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Mapk3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mapk3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mbp 17196
Biochemical Activity Mus musculus
2 Sqstm1  
Affinity Capture-Western Mus musculus
3 Map2k1 26395
Co-fractionation Mus musculus
4 Grin2b  
Affinity Capture-Western Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Mapk1 26413
Phenotypic Suppression Mus musculus
7 Shank3  
Affinity Capture-MS Mus musculus
8 Pea15a  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
9 Pea15b  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Co-localization Mus musculus
10 Agap2  
Affinity Capture-MS Mus musculus
11 Map2k2  
Co-fractionation Mus musculus
12 GABRR1  
Biochemical Activity Homo sapiens
13 Elk1  
Biochemical Activity Mus musculus
14 Syngap1  
Affinity Capture-MS Mus musculus
15 Nanog  
Two-hybrid Mus musculus
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
16 Rps6ka3 110651
Biochemical Activity Mus musculus
17 Grin1  
Affinity Capture-Western Mus musculus
18 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Mapk3 is involved
PathwayEvidenceSource
Activation of the AP-1 family of transcription factors IEA Reactome
Antiviral mechanism by IFN-stimulated genes IEA Reactome
Apoptosis IEA Reactome
Apoptotic factor-mediated response IEA Reactome
Axon guidance IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Cellular Senescence IEA Reactome
Cytochrome c-mediated apoptotic response IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Developmental Biology IEA Reactome
Downregulation of SMAD2/3:SMAD4 transcriptional activity IEA Reactome
ERK/MAPK targets IEA Reactome
ERKs are inactivated IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated MAPK activation IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
Formation of apoptosome IEA Reactome
Frs2-mediated activation IEA Reactome
G alpha (q) signalling events IEA Reactome
Gastrin-CREB signalling pathway via PKC and MAPK IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
Golgi Cisternae Pericentriolar Stack Reorganization IEA Reactome
GPCR downstream signalling IEA Reactome
Growth hormone receptor signaling IEA Reactome
Hemostasis IEA Reactome
IFNG signaling activates MAPKs IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Insulin receptor signalling cascade IEA Reactome
Interferon gamma signaling IEA Reactome
Interferon Signaling IEA Reactome
Interleukin-17 signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
Intrinsic Pathway for Apoptosis IEA Reactome
ISG15 antiviral mechanism IEA Reactome
L1CAM interactions IEA Reactome
M Phase IEA Reactome
MAP kinase activation IEA Reactome
MAP2K and MAPK activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK targets/ Nuclear events mediated by MAP kinases IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
MAPK3 (ERK1) activation IEA Reactome
MITF-M-regulated melanocyte development IEA Reactome
Mitotic Prophase IEA Reactome
MyD88 cascade initiated on plasma membrane IEA Reactome
MyD88 dependent cascade initiated on endosome IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane IEA Reactome
NCAM signaling for neurite out-growth IEA Reactome
Negative feedback regulation of MAPK pathway IEA Reactome
Negative regulation of FGFR1 signaling IEA Reactome
Negative regulation of FGFR2 signaling IEA Reactome
Negative regulation of FGFR3 signaling IEA Reactome
Negative regulation of FGFR4 signaling IEA Reactome
Negative regulation of MAPK pathway IEA Reactome
Negative regulation of the PI3K/AKT network IEA Reactome
Nervous system development IEA Reactome
Nuclear Events (kinase and transcription factor activation) IEA Reactome
Oncogene Induced Senescence IEA Reactome
Oxidative Stress Induced Senescence IEA Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Programmed Cell Death IEA Reactome
Prolonged ERK activation events IEA Reactome
RAF-independent MAPK1/3 activation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
Regulation of HSF1-mediated heat shock response IEA Reactome
Regulation of the apoptosome activity IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate NADPH Oxidases IEA Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
RNA Polymerase I Promoter Clearance IEA Reactome
RNA Polymerase I Promoter Opening IEA Reactome
RNA Polymerase I Transcription IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Senescence-Associated Secretory Phenotype (SASP) IEA Reactome
Signal attenuation IEA Reactome
Signal Transduction IEA Reactome
Signal transduction by L1 IEA Reactome
Signaling by Activin IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Insulin receptor IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by NODAL IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by TGF-beta Receptor Complex IEA Reactome
Signaling by TGFB family members IEA Reactome
Signalling to ERKs IEA Reactome
SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription IEA Reactome
Spry regulation of FGF signaling IEA Reactome
Thrombin signalling through proteinase activated receptors (PARs) IEA Reactome
Toll Like Receptor 10 (TLR10) Cascade IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade IEA Reactome
Toll Like Receptor 3 (TLR3) Cascade IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll Like Receptor 5 (TLR5) Cascade IEA Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade IEA Reactome
Toll Like Receptor 9 (TLR9) Cascade IEA Reactome
Toll Like Receptor TLR1:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR6:TLR2 Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation IEA Reactome
Transcriptional activity of SMAD2/SMAD3:SMAD4 heterotrimer IEA Reactome
Transcriptional and post-translational regulation of MITF-M expression and activity IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here