Experiment description of studies that identified Stat1 in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 263 MISEV standards ✘ Biophysical techniques ✔ CD63|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 25242146     Organism Mus musculus Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino" Journal name Molecular Cell Publication year 2014 Sample Neural stem cells Sample name NPCs - Th1 treated Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.20 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 264 MISEV standards ✘ Biophysical techniques ✔ CD63|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 25242146     Organism Mus musculus Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino" Journal name Molecular Cell Publication year 2014 Sample Neural stem cells Sample name NPCs - Th2 treated Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.20 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Stat1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Ubc   Affinity Capture-Western Mus musculus 2 Btrc   Affinity Capture-Western Mus musculus Affinity Capture-Western Mus musculus 3 SMURF2 64750 Affinity Capture-Luminescence Homo sapiens 4 Stat3 20848 Two-hybrid Mus musculus 5 Jak1 16451 Affinity Capture-Western Mus musculus 6 Fbxo2   Affinity Capture-Western Mus musculus 7 Rnf220   Affinity Capture-Western Mus musculus 8 Usp22   Affinity Capture-Western Mus musculus Biochemical Activity Mus musculus 9 Mllt10   Two-hybrid Mus musculus 10 SMURF1 57154 Affinity Capture-Luminescence Homo sapiens 11 SMAD4   Affinity Capture-Luminescence Homo sapiens 12 Ddb1 13194 Affinity Capture-MS Mus musculus 13 Stat1 20846 Affinity Capture-Western Mus musculus Affinity Capture-Western Mus musculus 14 ACVR1 90 Affinity Capture-Luminescence Homo sapiens 15 TGFBR1 7046 Affinity Capture-Luminescence Homo sapiens 16 Atf3   Affinity Capture-Western Mus musculus 17 Rnf19b   Affinity Capture-Western Mus musculus Affinity Capture-Western Mus musculus 18 Ifngr1 15979 Affinity Capture-Western Mus musculus 19 Mapk1 26413 Biochemical Activity Mus musculus Affinity Capture-Western Mus musculus 20 Myo6   Co-fractionation Mus musculus 21 Atg16l1   Affinity Capture-MS Mus musculus 22 Fancd2   Affinity Capture-MS Mus musculus View the network

Pathways in which Stat1 is involved

No pathways found