Gene description for Stat3
Gene name signal transducer and activator of transcription 3
Gene symbol Stat3
Other names/aliases 1110034C02Rik
AW109958
Aprf
Species Mus musculus
 Database cross references - Stat3
ExoCarta ExoCarta_20848
Vesiclepedia VP_20848
Entrez Gene 20848
UniProt P42227  
 Stat3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Stat3
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    transcription cis-regulatory region binding GO:0000976 ISO
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IEA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISO
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IEA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 ISO
    DNA binding GO:0003677 IDA
    DNA binding GO:0003677 ISO
    DNA-binding transcription factor activity GO:0003700 IMP
    DNA-binding transcription factor activity GO:0003700 ISO
    nuclear receptor activity GO:0004879 IEA
    nuclear receptor activity GO:0004879 ISO
    signaling receptor binding GO:0005102 IEA
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    protein phosphatase binding GO:0019903 IEA
    protein phosphatase binding GO:0019903 ISO
    chromatin DNA binding GO:0031490 ISO
    chromatin DNA binding GO:0031490 ISS
    CCR5 chemokine receptor binding GO:0031730 ISO
    nuclear glucocorticoid receptor binding GO:0035259 ISO
    signaling adaptor activity GO:0035591 IEA
    signaling adaptor activity GO:0035591 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IDA
    protein homodimerization activity GO:0042803 ISO
    sequence-specific DNA binding GO:0043565 IDA
    sequence-specific DNA binding GO:0043565 ISO
    protein dimerization activity GO:0046983 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
    primary miRNA binding GO:0070878 IEA
    primary miRNA binding GO:0070878 ISO
    lncRNA binding GO:0106222 IEA
    lncRNA binding GO:0106222 ISO
    acetylation-dependent protein binding GO:0140033 ISS
    DNA-binding transcription factor binding GO:0140297 ISO
    DNA-binding transcription factor binding GO:0140297 ISS
    RNA sequestering activity GO:0140610 IEA
    RNA sequestering activity GO:0140610 ISO
Biological Process
    temperature homeostasis GO:0001659 IMP
    response to ischemia GO:0002931 ISO
    regulation of DNA-templated transcription GO:0006355 ISO
    regulation of DNA-templated transcription GO:0006355 NAS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    transcription by RNA polymerase II GO:0006366 IDA
    transcription by RNA polymerase II GO:0006366 IGI
    protein import into nucleus GO:0006606 ISO
    protein import into nucleus GO:0006606 ISS
    defense response GO:0006952 IBA
    acute-phase response GO:0006953 IEA
    inflammatory response GO:0006954 IMP
    transforming growth factor beta receptor signaling pathway GO:0007179 IEA
    transforming growth factor beta receptor signaling pathway GO:0007179 ISO
    cell surface receptor signaling pathway via JAK-STAT GO:0007259 IBA
    cell surface receptor signaling pathway via JAK-STAT GO:0007259 ISO
    cell surface receptor signaling pathway via JAK-STAT GO:0007259 TAS
    cell population proliferation GO:0008283 IDA
    positive regulation of cell population proliferation GO:0008284 ISO
    negative regulation of cell population proliferation GO:0008285 IGI
    gene expression GO:0010467 IMP
    negative regulation of autophagy GO:0010507 IEA
    negative regulation of autophagy GO:0010507 ISO
    positive regulation of vascular endothelial growth factor production GO:0010575 IEA
    positive regulation of vascular endothelial growth factor production GO:0010575 ISO
    positive regulation of gene expression GO:0010628 IGI
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of gene expression GO:0010629 ISO
    negative regulation of hydrogen peroxide biosynthetic process GO:0010730 ISO
    phosphorylation GO:0016310 IDA
    cytokine-mediated signaling pathway GO:0019221 NAS
    sexual reproduction GO:0019953 IMP
    cell differentiation GO:0030154 ISO
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell migration GO:0030335 ISO
    intracellular receptor signaling pathway GO:0030522 IEA
    intracellular receptor signaling pathway GO:0030522 ISO
    response to estradiol GO:0032355 IEA
    response to estradiol GO:0032355 ISO
    positive regulation of interleukin-1 beta production GO:0032731 IEA
    positive regulation of interleukin-1 beta production GO:0032731 ISO
    positive regulation of interleukin-10 production GO:0032733 IEA
    positive regulation of interleukin-10 production GO:0032733 ISO
    positive regulation of interleukin-6 production GO:0032755 ISO
    positive regulation of interleukin-8 production GO:0032757 IEA
    positive regulation of interleukin-8 production GO:0032757 ISO
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    cellular response to hormone stimulus GO:0032870 ISO
    leptin-mediated signaling pathway GO:0033210 IBA
    leptin-mediated signaling pathway GO:0033210 IDA
    leptin-mediated signaling pathway GO:0033210 IMP
    leptin-mediated signaling pathway GO:0033210 ISO
    leptin-mediated signaling pathway GO:0033210 ISS
    response to cytokine GO:0034097 ISO
    somatic stem cell population maintenance GO:0035019 IMP
    interleukin-15-mediated signaling pathway GO:0035723 IEA
    interleukin-15-mediated signaling pathway GO:0035723 ISO
    interleukin-2-mediated signaling pathway GO:0038110 IEA
    interleukin-2-mediated signaling pathway GO:0038110 ISO
    interleukin-9-mediated signaling pathway GO:0038113 IEA
    interleukin-9-mediated signaling pathway GO:0038113 ISO
    interleukin-11-mediated signaling pathway GO:0038154 IEA
    interleukin-11-mediated signaling pathway GO:0038154 ISO
    interleukin-23-mediated signaling pathway GO:0038155 IEA
    regulation of multicellular organism growth GO:0040014 IMP
    regulation of cell population proliferation GO:0042127 IBA
    glucose homeostasis GO:0042593 IMP
    glucose homeostasis GO:0042593 IMP
    eating behavior GO:0042755 IMP
    mRNA transcription by RNA polymerase II GO:0042789 IDA
    response to peptide hormone GO:0043434 IBA
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IDA
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 ISO
    cellular response to leptin stimulus GO:0044320 ISO
    cellular response to leptin stimulus GO:0044320 ISS
    response to leptin GO:0044321 IDA
    response to leptin GO:0044321 IMP
    response to leptin GO:0044321 ISO
    response to leptin GO:0044321 ISS
    positive regulation of erythrocyte differentiation GO:0045648 ISO
    positive regulation of erythrocyte differentiation GO:0045648 ISS
    positive regulation of Notch signaling pathway GO:0045747 IMP
    positive regulation of angiogenesis GO:0045766 IDA
    negative regulation of glycolytic process GO:0045820 IMP
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of DNA-templated transcription GO:0045893 IMP
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    regulation of mitochondrial membrane permeability GO:0046902 ISO
    astrocyte differentiation GO:0048708 IMP
    negative regulation of inflammatory response GO:0050728 IDA
    modulation of chemical synaptic transmission GO:0050804 ISO
    regulation of cell cycle GO:0051726 IDA
    regulation of cell cycle GO:0051726 ISO
    regulation of cell cycle GO:0051726 ISS
    radial glial cell differentiation GO:0060019 IMP
    retinal rod cell differentiation GO:0060221 IMP
    regulation of feeding behavior GO:0060259 IMP
    growth hormone receptor signaling pathway GO:0060396 ISO
    growth hormone receptor signaling pathway via JAK-STAT GO:0060397 IBA
    growth hormone receptor signaling pathway via JAK-STAT GO:0060397 IDA
    growth hormone receptor signaling pathway via JAK-STAT GO:0060397 ISO
    interleukin-6-mediated signaling pathway GO:0070102 ISO
    interleukin-6-mediated signaling pathway GO:0070102 ISS
    cellular response to cytokine stimulus GO:0071345 ISO
    cellular response to organic cyclic compound GO:0071407 ISO
    T-helper 17 type immune response GO:0072538 ISO
    T-helper 17 type immune response GO:0072538 ISS
    T-helper 17 cell lineage commitment GO:0072540 IMP
    energy homeostasis GO:0097009 IMP
    cellular response to interleukin-17 GO:0097398 IDA
    cell surface receptor signaling pathway via STAT GO:0097696 ISO
    postsynapse to nucleus signaling pathway GO:0099527 ISO
    negative regulation of inflammatory response to wounding GO:0106015 IDA
    negative regulation of inflammatory response to wounding GO:0106015 ISO
    interleukin-10-mediated signaling pathway GO:0140105 IEA
    interleukin-10-mediated signaling pathway GO:0140105 ISO
    positive regulation of cytokine production involved in inflammatory response GO:1900017 IEA
    positive regulation of cytokine production involved in inflammatory response GO:1900017 ISO
    regulation of cellular response to hypoxia GO:1900037 ISO
    positive regulation of growth factor dependent skeletal muscle satellite cell proliferation GO:1902728 ISO
    positive regulation of miRNA transcription GO:1902895 IMP
    positive regulation of miRNA transcription GO:1902895 ISO
    positive regulation of metalloendopeptidase activity GO:1904685 ISO
    positive regulation of vascular endothelial cell proliferation GO:1905564 IDA
    negative regulation of primary miRNA processing GO:2000635 IEA
    negative regulation of primary miRNA processing GO:2000635 ISO
    negative regulation of stem cell differentiation GO:2000737 IMP
    positive regulation of ATP biosynthetic process GO:2001171 ISO
    negative regulation of neuron migration GO:2001223 IGI
Subcellular Localization
    chromatin GO:0000785 IEA
    chromatin GO:0000785 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    transcription regulator complex GO:0005667 IDA
    transcription regulator complex GO:0005667 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrial inner membrane GO:0005743 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    postsynaptic density GO:0014069 ISO
    protein-containing complex GO:0032991 IDA
    RNA polymerase II transcription regulator complex GO:0090575 IBA
    RNA polymerase II transcription regulator complex GO:0090575 IEA
    RNA polymerase II transcription regulator complex GO:0090575 ISO
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Stat3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Stat3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Csf1r  
Co-fractionation Mus musculus
2 CRTC2 200186
Affinity Capture-Western Homo sapiens
3 Stat3 20848
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
4 Il6st 16195
Co-fractionation Mus musculus
5 Irf4  
Co-localization Mus musculus
6 Nfyb  
Co-fractionation Mus musculus
7 Cyld  
Affinity Capture-Western Mus musculus
8 ABCG1  
Affinity Capture-MS Homo sapiens
9 Pou6f1  
Two-hybrid Mus musculus
10 Tmem173  
Proximity Label-MS Mus musculus
11 Ndufa13  
Co-fractionation Mus musculus
12 Foxp3  
Affinity Capture-MS Mus musculus
13 PIAS3  
Affinity Capture-Western Homo sapiens
14 Jun  
Two-hybrid Mus musculus
15 Pcbp1 23983
Affinity Capture-Western Mus musculus
Phenotypic Enhancement Mus musculus
16 Stat1 20846
Two-hybrid Mus musculus
17 Jak3  
Affinity Capture-Western Mus musculus
18 Jak2 16452
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
19 CRTC3  
Affinity Capture-Western Homo sapiens
20 Tmf1  
Affinity Capture-Western Mus musculus
21 Il1rap 16180
Co-fractionation Mus musculus
22 Eed  
Affinity Capture-MS Mus musculus
23 Usp22  
Biochemical Activity Mus musculus
24 Irak1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
25 Stat2  
Affinity Capture-Western Mus musculus
26 Arid1b 239985
Affinity Capture-Western Mus musculus
27 EP300 2033
Affinity Capture-Western Homo sapiens
28 Pgr  
Affinity Capture-Western Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here