Gene description for PRAF2
Gene name PRA1 domain family, member 2
Gene symbol PRAF2
Other names/aliases JM4
Species Homo sapiens
 Database cross references - PRAF2
ExoCarta ExoCarta_11230
Vesiclepedia VP_11230
Entrez Gene 11230
HGNC 28911
MIM 300840
UniProt O60831  
 PRAF2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PRAF2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    protein transport GO:0015031 IEA
    L-glutamate transmembrane transport GO:0015813 ISS
Subcellular Localization
    endosome membrane GO:0010008 IEA
    membrane GO:0016020 IBA
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
    GABA-ergic synapse GO:0098982 IEA
 Experiment description of studies that identified PRAF2 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PRAF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 VPS53 55275
Affinity Capture-MS Homo sapiens
3 DUSP22  
Affinity Capture-MS Homo sapiens
4 HSD17B11 51170
Two-hybrid Homo sapiens
5 GJA8  
Two-hybrid Homo sapiens
6 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
7 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
8 CYB5B 80777
Affinity Capture-MS Homo sapiens
9 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
10 SLC10A6  
Two-hybrid Homo sapiens
11 PDE2A  
Affinity Capture-MS Homo sapiens
12 REEP5 7905
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 BCAP31 10134
Proximity Label-MS Homo sapiens
14 GJA5 2702
Two-hybrid Homo sapiens
15 EBAG9 9166
Two-hybrid Homo sapiens
16 MARCKS 4082
Proximity Label-MS Homo sapiens
17 LAMP3  
Proximity Label-MS Homo sapiens
18 LMNB1 4001
Proximity Label-MS Homo sapiens
19 LMAN1 3998
Proximity Label-MS Homo sapiens
20 VAC14 55697
Affinity Capture-MS Homo sapiens
21 ARL6IP5 10550
Cross-Linking-MS (XL-MS) Homo sapiens
22 E2F4  
Affinity Capture-MS Homo sapiens
23 MCEMP1  
Two-hybrid Homo sapiens
24 HMOX2 3163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 FAM96A  
Two-hybrid Homo sapiens
26 RP2 6102
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TMEM206 55248
Affinity Capture-MS Homo sapiens
28 TMEM14B  
Two-hybrid Homo sapiens
29 HSD3B7 80270
Proximity Label-MS Homo sapiens
30 TSPAN17 26262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 COPB2 9276
Affinity Capture-MS Homo sapiens
32 MCM10 55388
Affinity Capture-MS Homo sapiens
33 ABHD16A 7920
Two-hybrid Homo sapiens
34 LAMP2 3920
Proximity Label-MS Homo sapiens
35 DPEP1 1800
Affinity Capture-MS Homo sapiens
36 TSPAN10 83882
Affinity Capture-MS Homo sapiens
37 REEP4  
Two-hybrid Homo sapiens
38 AGR3 155465
Two-hybrid Homo sapiens
39 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 FAM63B 54629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 YIPF5 81555
Affinity Capture-MS Homo sapiens
42 GPR133  
Two-hybrid Homo sapiens
43 RTN4 57142
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 LPAR1 1902
Affinity Capture-MS Homo sapiens
45 SEC61B 10952
Proximity Label-MS Homo sapiens
46 NDRG4 65009
Two-hybrid Homo sapiens
47 GPR152  
Two-hybrid Homo sapiens
48 TSPAN5 10098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
50 MAPK6  
Affinity Capture-MS Homo sapiens
51 B3GAT1  
Proximity Label-MS Homo sapiens
52 FAM134C 162427
Two-hybrid Homo sapiens
53 CD79A  
Two-hybrid Homo sapiens
54 RNF5  
Two-hybrid Homo sapiens
55 SEC62 7095
Proximity Label-MS Homo sapiens
56 ATP2A1 487
Proximity Label-MS Homo sapiens
57 HIBADH 11112
Two-hybrid Homo sapiens
58 ARF6 382
Proximity Label-MS Homo sapiens
59 RPN2 6185
Proximity Label-MS Homo sapiens
60 RAB1A 5861
Affinity Capture-MS Homo sapiens
61 DUSP9  
Affinity Capture-MS Homo sapiens
62 TNFRSF10C  
Affinity Capture-MS Homo sapiens
63 RPN1 6184
Proximity Label-MS Homo sapiens
64 DUSP21  
Affinity Capture-MS Homo sapiens
65 RAB5C 5878
Proximity Label-MS Homo sapiens
66 ERGIC1 57222
Proximity Label-MS Homo sapiens
67 TMEM56  
Two-hybrid Homo sapiens
68 DDRGK1 65992
Affinity Capture-MS Homo sapiens
69 IFITM3 10410
Affinity Capture-MS Homo sapiens
70 CKAP4 10970
Proximity Label-MS Homo sapiens
71 DERL1 79139
Proximity Label-MS Homo sapiens
72 RPA3 6119
Proximity Label-MS Homo sapiens
73 CAV1 857
Proximity Label-MS Homo sapiens
74 ELOVL5 60481
Proximity Label-MS Homo sapiens
75 GJD3  
Proximity Label-MS Homo sapiens
76 ZDHHC15 158866
Two-hybrid Homo sapiens
77 COIL  
Proximity Label-MS Homo sapiens
78 MECP2 4204
Affinity Capture-MS Homo sapiens
79 TMX2 51075
Two-hybrid Homo sapiens
80 SLC7A1 6541
Affinity Capture-MS Homo sapiens
81 CLEC10A  
Two-hybrid Homo sapiens
82 TM4SF5  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PRAF2 is involved
No pathways found





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