Gene description for Ap1g1
Gene name adaptor protein complex AP-1, gamma 1 subunit
Gene symbol Ap1g1
Other names/aliases AA409002
AU041323
AW551707
Adtg
D8Ertd374e
Species Mus musculus
 Database cross references - Ap1g1
ExoCarta ExoCarta_11765
Vesiclepedia VP_11765
Entrez Gene 11765
UniProt P22892  
 Ap1g1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ap1g1
Molecular Function
    protein binding GO:0005515 IPI
    kinesin binding GO:0019894 ISO
    GTP-dependent protein binding GO:0030742 ISO
    small GTPase binding GO:0031267 ISO
    clathrin adaptor activity GO:0035615 IBA
Biological Process
    intracellular protein transport GO:0006886 TAS
    Golgi to vacuole transport GO:0006896 IBA
    vesicle-mediated transport GO:0016192 NAS
    vesicle-mediated transport GO:0016192 TAS
    endosome to melanosome transport GO:0035646 ISO
    positive regulation of natural killer cell degranulation GO:0043323 ISO
    positive regulation of natural killer cell mediated cytotoxicity GO:0045954 ISO
    platelet dense granule organization GO:0060155 NAS
    Golgi to lysosome transport GO:0090160 ISO
    basolateral protein secretion GO:0110010 NAS
    melanosome assembly GO:1903232 NAS
Subcellular Localization
    lysosomal membrane GO:0005765 NAS
    early endosome GO:0005769 NAS
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 IDA
    trans-Golgi network GO:0005802 TAS
    cytosol GO:0005829 ISO
    clathrin-coated pit GO:0005905 IEA
    AP-1 adaptor complex GO:0030121 IBA
    AP-1 adaptor complex GO:0030121 NAS
    clathrin-coated vesicle GO:0030136 ISO
    trans-Golgi network membrane GO:0032588 NAS
    intracellular membrane-bounded organelle GO:0043231 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    recycling endosome GO:0055037 ISO
    presynapse GO:0098793 IDA
 Experiment description of studies that identified Ap1g1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ap1g1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rab5a 271457
Co-fractionation Mus musculus
2 Necap2  
Co-fractionation Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Ubl7  
Co-fractionation Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Atg5  
Affinity Capture-MS Mus musculus
7 Rab14 68365
Co-fractionation Mus musculus
8 Aftph  
Co-fractionation Mus musculus
9 Unk  
Affinity Capture-RNA Mus musculus
10 ABCG1  
Affinity Capture-MS Homo sapiens
11 Synrg  
Co-fractionation Mus musculus
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