 Experiment description of studies that identified Rab5a in sEVs |
| 1 |
| Experiment ID |
907 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Colon cancer cells |
| Sample name |
C26 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 2 |
| Experiment ID |
15 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
17486113
|
| Organism |
Mus musculus
Homo sapiens |
| Experiment description |
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. |
| Authors |
"Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" |
| Journal name |
NCB
|
| Publication year |
2007 |
| Sample |
Mast cells |
| Sample name |
MC9
Bone marrow-derived mast cells
HMC-1 |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.11-1.21 g/mL
|
| Molecules identified in the study |
Protein
mRNA
miRNA |
| Methods used in the study |
Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array |
|
|
| 3 |
| Experiment ID |
39 |
| MISEV standards |
|
✔
IEM
|
Biophysical techniques |
|
✔
TSG101|HSC70|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
15210972
|
| Organism |
Mus musculus |
| Experiment description |
Cells release prions in association with exosomes. |
| Authors |
"Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G" |
| Journal name |
PNAS
|
| Publication year |
2004 |
| Sample |
Mov neuroglial cells |
| Sample name |
Mov neuroglial cell |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.14 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy |
|
|
| 4 |
| Experiment ID |
908 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
T-cell lymphoma cells |
| Sample name |
EL4 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
