Gene description for Rab14
Gene name RAB14, member RAS oncogene family
Gene symbol Rab14
Other names/aliases 0610030G24Rik
2810475J17Rik
A830021G03Rik
AI314285
AI649155
D030017L14Rik
Species Mus musculus
 Database cross references - Rab14
ExoCarta ExoCarta_68365
Vesiclepedia VP_68365
Entrez Gene 68365
UniProt Q91V41  
 Rab14 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab14
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    GTPase activity GO:0003924 TAS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
    myosin V binding GO:0031489 IEA
    myosin V binding GO:0031489 ISO
Biological Process
    phagolysosome assembly GO:0001845 IBA
    intracellular protein transport GO:0006886 IBA
    Golgi to endosome transport GO:0006895 IBA
    Golgi to endosome transport GO:0006895 IMP
    body fluid secretion GO:0007589 ISO
    fibroblast growth factor receptor signaling pathway GO:0008543 IMP
    protein transport GO:0015031 IEA
    endocytic recycling GO:0032456 ISO
    endocytic recycling GO:0032456 ISS
    regulation of protein localization GO:0032880 ISO
    regulation of protein localization GO:0032880 ISS
    endosomal vesicle fusion GO:0034058 IEA
    endosomal vesicle fusion GO:0034058 ISO
    early endosome to Golgi transport GO:0034498 IEA
    early endosome to Golgi transport GO:0034498 ISO
    defense response to bacterium GO:0042742 IEA
    apical protein localization GO:0045176 ISO
    regulation of embryonic development GO:0045995 IMP
    phagosome maturation GO:0090382 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    lysosome GO:0005764 ISO
    early endosome GO:0005769 IBA
    early endosome GO:0005769 ISO
    late endosome GO:0005770 ISO
    rough endoplasmic reticulum GO:0005791 ISO
    Golgi stack GO:0005795 ISO
    trans-Golgi network GO:0005802 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    endomembrane system GO:0012505 IBA
    apical plasma membrane GO:0016324 ISO
    trans-Golgi network transport vesicle GO:0030140 ISO
    cytoplasmic vesicle membrane GO:0030659 TAS
    synaptic vesicle membrane GO:0030672 ISO
    early endosome membrane GO:0031901 IEA
    early endosome membrane GO:0031901 ISO
    nuclear outer membrane-endoplasmic reticulum membrane network GO:0042175 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    phagocytic vesicle GO:0045335 IBA
    phagocytic vesicle GO:0045335 ISO
    phagocytic vesicle GO:0045335 ISS
    perinuclear region of cytoplasm GO:0048471 ISO
    recycling endosome GO:0055037 ISO
    recycling endosome GO:0055037 ISS
    alveolar lamellar body GO:0097208 ISO
 Experiment description of studies that identified Rab14 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Tsc1  
Affinity Capture-MS Mus musculus
3 Cat 12359
Co-fractionation Mus musculus
4 Ap1g1 11765
Co-fractionation Mus musculus
5 Eea1  
Co-fractionation Mus musculus
6 SMURF2 64750
Affinity Capture-Luminescence Homo sapiens
7 Ocrl  
Co-fractionation Mus musculus
8 Dlg4  
Affinity Capture-MS Mus musculus
9 Tia1  
Affinity Capture-MS Mus musculus
10 Rab5a 271457
Co-fractionation Mus musculus
11 Rab11fip5  
Co-fractionation Mus musculus
12 Unk  
Affinity Capture-RNA Mus musculus
13 TBC1D4  
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
14 Fancd2  
Affinity Capture-MS Mus musculus
15 TBC1D1 23216
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
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