Gene description for Cat
Gene name catalase
Gene symbol Cat
Other names/aliases 2210418N07
Cas-1
Cas1
Cs-1
Species Mus musculus
 Database cross references - Cat
ExoCarta ExoCarta_12359
Vesiclepedia VP_12359
Entrez Gene 12359
UniProt P24270  
 Cat identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cat
Molecular Function
    aminoacylase activity GO:0004046 IMP
    catalase activity GO:0004096 IBA
    catalase activity GO:0004096 IDA
    catalase activity GO:0004096 IMP
    catalase activity GO:0004096 ISO
    antioxidant activity GO:0016209 ISO
    oxidoreductase activity, acting on peroxide as acceptor GO:0016684 IDA
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    heme binding GO:0020037 IBA
    heme binding GO:0020037 IEA
    heme binding GO:0020037 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    metal ion binding GO:0046872 IEA
    NADP binding GO:0050661 IEA
    NADP binding GO:0050661 ISO
Biological Process
    response to reactive oxygen species GO:0000302 ISO
    ureteric bud development GO:0001657 IEA
    ureteric bud development GO:0001657 ISO
    response to hypoxia GO:0001666 IEA
    response to hypoxia GO:0001666 ISO
    kidney development GO:0001822 ISO
    triglyceride metabolic process GO:0006641 IMP
    response to oxidative stress GO:0006979 IDA
    response to oxidative stress GO:0006979 IMP
    response to oxidative stress GO:0006979 ISO
    cholesterol metabolic process GO:0008203 IMP
    aerobic respiration GO:0009060 IMP
    response to xenobiotic stimulus GO:0009410 IEA
    response to light intensity GO:0009642 IEA
    UV protection GO:0009650 IEA
    UV protection GO:0009650 ISO
    response to ozone GO:0010193 IEA
    response to lead ion GO:0010288 IEA
    response to activity GO:0014823 IEA
    response to inactivity GO:0014854 IEA
    hemoglobin metabolic process GO:0020027 IMP
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IDA
    response to estradiol GO:0032355 IEA
    response to insulin GO:0032868 IEA
    response to vitamin A GO:0033189 IEA
    response to vitamin E GO:0033197 IEA
    response to L-ascorbic acid GO:0033591 IEA
    response to hydrogen peroxide GO:0042542 IBA
    hydrogen peroxide catabolic process GO:0042744 IBA
    hydrogen peroxide catabolic process GO:0042744 IDA
    hydrogen peroxide catabolic process GO:0042744 IMP
    hydrogen peroxide catabolic process GO:0042744 ISO
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    response to ethanol GO:0045471 IEA
    response to cadmium ion GO:0046686 IEA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    positive regulation of cell division GO:0051781 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    response to hyperoxia GO:0055093 IEA
    cellular detoxification of hydrogen peroxide GO:0061692 ISO
    response to fatty acid GO:0070542 IEA
    cellular response to growth factor stimulus GO:0071363 IEA
    response to amitrole GO:0072722 IEA
    response to phenylpropanoid GO:0080184 IEA
Subcellular Localization
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 IDA
    peroxisome GO:0005777 ISO
    peroxisome GO:0005777 ISO
    peroxisomal membrane GO:0005778 IDA
    peroxisomal matrix GO:0005782 IEA
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    catalase complex GO:0062151 ISO
 Experiment description of studies that identified Cat in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cat
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ehhadh  
Co-fractionation Mus musculus
2 Cat 12359
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
3 Pex14  
Co-fractionation Mus musculus
4 Rab14 68365
Co-fractionation Mus musculus
5 HSD17B10 3028
Two-hybrid Homo sapiens
6 Dhrs4  
Co-fractionation Mus musculus
7 Zadh2  
Co-fractionation Mus musculus
8 Acad11  
Co-fractionation Mus musculus
9 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here