Gene description for Cdk6
Gene name cyclin-dependent kinase 6
Gene symbol Cdk6
Other names/aliases 5830411I20
AI504062
Crk2
Species Mus musculus
 Database cross references - Cdk6
ExoCarta ExoCarta_12571
Vesiclepedia VP_12571
Entrez Gene 12571
UniProt Q64261  
 Cdk6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cdk6
Molecular Function
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IBA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IEA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    kinase activity GO:0016301 IDA
    cyclin binding GO:0030332 IBA
    cyclin binding GO:0030332 IEA
    cyclin binding GO:0030332 ISO
    FBXO family protein binding GO:0098770 IEA
    FBXO family protein binding GO:0098770 ISO
    protein serine kinase activity GO:0106310 IEA
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 IBA
    G1/S transition of mitotic cell cycle GO:0000082 IEA
    G1/S transition of mitotic cell cycle GO:0000082 ISO
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    positive regulation of cell-matrix adhesion GO:0001954 IEA
    positive regulation of cell-matrix adhesion GO:0001954 ISO
    hematopoietic progenitor cell differentiation GO:0002244 IMP
    type B pancreatic cell development GO:0003323 IEA
    type B pancreatic cell development GO:0003323 ISO
    protein phosphorylation GO:0006468 ISO
    DNA damage response GO:0006974 IEA
    DNA damage response GO:0006974 ISO
    signal transduction GO:0007165 IBA
    Notch signaling pathway GO:0007219 IDA
    Notch signaling pathway GO:0007219 IGI
    response to virus GO:0009615 IEA
    regulation of G2/M transition of mitotic cell cycle GO:0010389 IBA
    regulation of gene expression GO:0010468 IBA
    regulation of gene expression GO:0010468 ISO
    positive regulation of gene expression GO:0010628 IDA
    hemopoiesis GO:0030097 IMP
    T cell differentiation in thymus GO:0033077 IMP
    response to 5-fluorouracil GO:0036275 IMP
    gliogenesis GO:0042063 IEA
    gliogenesis GO:0042063 ISO
    cell dedifferentiation GO:0043697 IEA
    cell dedifferentiation GO:0043697 ISO
    cell quiescence GO:0044838 IMP
    negative regulation of myeloid cell differentiation GO:0045638 ISO
    regulation of erythrocyte differentiation GO:0045646 IEA
    regulation of erythrocyte differentiation GO:0045646 ISO
    negative regulation of monocyte differentiation GO:0045656 ISO
    negative regulation of monocyte differentiation GO:0045656 ISS
    negative regulation of osteoblast differentiation GO:0045668 IEA
    negative regulation of osteoblast differentiation GO:0045668 ISO
    negative regulation of cell cycle GO:0045786 IEA
    negative regulation of cell cycle GO:0045786 ISO
    positive regulation of fibroblast proliferation GO:0048146 IEA
    positive regulation of fibroblast proliferation GO:0048146 ISO
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    negative regulation of epithelial cell proliferation GO:0050680 ISO
    cell division GO:0051301 IEA
    hematopoietic stem cell differentiation GO:0060218 IMP
    regulation of hematopoietic stem cell differentiation GO:1902036 IMP
    negative regulation of cellular senescence GO:2000773 IEA
    negative regulation of cellular senescence GO:2000773 ISO
Subcellular Localization
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IBA
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 ISO
    ruffle GO:0001726 IEA
    ruffle GO:0001726 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cyclin D1-CDK6 complex GO:0097131 IEA
    cyclin D1-CDK6 complex GO:0097131 ISO
    cyclin D2-CDK6 complex GO:0097132 IDA
    cyclin D3-CDK6 complex GO:0097133 IEA
    cyclin D3-CDK6 complex GO:0097133 ISO
 Experiment description of studies that identified Cdk6 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cdk6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cdkn1b  
Affinity Capture-Western Mus musculus
2 Hist1h1a  
Biochemical Activity Mus musculus
3 Kif26b  
Co-fractionation Mus musculus
4 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
5 Dmbt1 12945
Co-fractionation Mus musculus
6 Foxp3  
Affinity Capture-MS Mus musculus
7 Runx1 12394
Affinity Capture-MS Mus musculus
8 Ccnd2 12444
Affinity Capture-Western Mus musculus
9 Mmrn1  
Co-fractionation Mus musculus
10 Ccnd3  
Affinity Capture-Western Mus musculus
11 Rb1  
Biochemical Activity Mus musculus
12 Ccnd1  
Affinity Capture-Western Mus musculus
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