Gene description for CTNNA2
Gene name catenin (cadherin-associated protein), alpha 2
Gene symbol CTNNA2
Other names/aliases CAP-R
CAPR
CT114
CTNR
Species Homo sapiens
 Database cross references - CTNNA2
ExoCarta ExoCarta_1496
Vesiclepedia VP_1496
Entrez Gene 1496
HGNC 2510
MIM 114025
UniProt P26232  
 CTNNA2 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for CTNNA2
Molecular Function
    structural constituent of cytoskeleton GO:0005200 NAS
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IBA
    cadherin binding GO:0045296 HDA
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
Biological Process
    cytoskeleton organization GO:0007010 IEA
    axonogenesis GO:0007409 ISS
    regulation of neuron projection development GO:0010975 IMP
    cell migration GO:0016477 IBA
    radial glia guided migration of Purkinje cell GO:0021942 ISS
    negative regulation of Arp2/3 complex-mediated actin nucleation GO:0034316 IMP
    dendrite morphogenesis GO:0048813 ISS
    brain morphogenesis GO:0048854 ISS
    regulation of synapse structural plasticity GO:0051823 ISS
    prepulse inhibition GO:0060134 ISS
    cell-cell adhesion GO:0098609 IBA
    cell-cell adhesion GO:0098609 ISS
    regulation of neuron migration GO:2001222 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    adherens junction GO:0005912 IBA
    adherens junction GO:0005912 ISS
    actin cytoskeleton GO:0015629 IEA
    catenin complex GO:0016342 IBA
    axon GO:0030424 ISS
 Experiment description of studies that identified CTNNA2 in sEVs
1
Experiment ID 207
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 257
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 259
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 260
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
10
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CTNNA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SDHAF2 54949
Affinity Capture-MS Homo sapiens
2 RYK 6259
Affinity Capture-MS Homo sapiens
3 DUSP22  
Affinity Capture-MS Homo sapiens
4 CIR1  
Affinity Capture-MS Homo sapiens
5 SLIRP 81892
Affinity Capture-MS Homo sapiens
6 GAN 8139
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 FBXW11  
Affinity Capture-MS Homo sapiens
9 UPK2 7379
Affinity Capture-MS Homo sapiens
10 Tyw3  
Affinity Capture-MS Mus musculus
11 PCDH20  
Affinity Capture-MS Homo sapiens
12 JUP 3728
Co-fractionation Homo sapiens
13 PCDHA8  
Affinity Capture-MS Homo sapiens
14 FBXL5  
Affinity Capture-MS Homo sapiens
15 CDH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CTNNB1 1499
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
17 FBXO45 200933
Affinity Capture-MS Homo sapiens
18 CLEC4A  
Affinity Capture-MS Homo sapiens
19 EGFR 1956
Affinity Capture-MS Homo sapiens
20 MYC  
Affinity Capture-MS Homo sapiens
21 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
22 UTP3 57050
Affinity Capture-MS Homo sapiens
23 MCAM 4162
Proximity Label-MS Homo sapiens
24 CD27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TAX1BP3 30851
Affinity Capture-MS Homo sapiens
26 CDH1 999
Proximity Label-MS Homo sapiens
27 SAPCD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CDH8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 NAAA  
Affinity Capture-MS Homo sapiens
30 NTRK1 4914
Affinity Capture-MS Homo sapiens
31 MCM2 4171
Affinity Capture-MS Homo sapiens
32 CLEC2B  
Affinity Capture-MS Homo sapiens
33 PVR 5817
Affinity Capture-MS Homo sapiens
34 NXPH3  
Affinity Capture-MS Homo sapiens
35 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
36 CTNNA1 1495
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
38 CDH12  
Affinity Capture-MS Homo sapiens
39 CTNNBIP1 56998
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 RBBP8  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CTNNA2 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Developmental Biology IEA Reactome
Myogenesis TAS Reactome
Myogenesis IEA Reactome





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