Gene description for DGKH
Gene name diacylglycerol kinase, eta
Gene symbol DGKH
Other names/aliases DGKeta
Species Homo sapiens
 Database cross references - DGKH
ExoCarta ExoCarta_160851
Vesiclepedia VP_160851
Entrez Gene 160851
HGNC 2854
MIM 604071
UniProt Q86XP1  
 DGKH identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DGKH
Molecular Function
    ATP-dependent diacylglycerol kinase activity GO:0004143 IBA
    ATP-dependent diacylglycerol kinase activity GO:0004143 IDA
    ATP-dependent diacylglycerol kinase activity GO:0004143 IMP
    ATP-dependent diacylglycerol kinase activity GO:0004143 ISS
    ATP-dependent diacylglycerol kinase activity GO:0004143 TAS
    ATP binding GO:0005524 IEA
    kinase binding GO:0019900 IPI
    SAM domain binding GO:0032093 IDA
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    phosphatidic acid biosynthetic process GO:0006654 IBA
    phosphatidic acid biosynthetic process GO:0006654 ISS
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IEA
    platelet activation GO:0030168 TAS
    intracellular signal transduction GO:0035556 IBA
    diacylglycerol metabolic process GO:0046339 IBA
    diacylglycerol metabolic process GO:0046339 ISS
    phosphatidic acid metabolic process GO:0046473 IDA
    lipid phosphorylation GO:0046834 ISS
    phospholipase C/protein kinase C signal transduction GO:0141212 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    endosome GO:0005768 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    actin cytoskeleton GO:0015629 ISS
    protein-containing complex GO:0032991 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified DGKH in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DGKH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 REEP5 7905
Affinity Capture-MS Homo sapiens
3 LINC01587  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PRKY  
Affinity Capture-MS Homo sapiens
5 KIF21A 55605
Affinity Capture-MS Homo sapiens
6 DGKG  
Affinity Capture-MS Homo sapiens
7 S100A2 6273
Affinity Capture-MS Homo sapiens
8 MTCH2 23788
Proximity Label-MS Homo sapiens
9 ERGIC1 57222
Proximity Label-MS Homo sapiens
10 MAGIX 79917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 DGKD 8527
Affinity Capture-Western Homo sapiens
12 BRCA2 675
Affinity Capture-MS Homo sapiens
13 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
14 ARRB1 408
Affinity Capture-MS Homo sapiens
15 DGKH 160851
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 TRAF5  
Two-hybrid Homo sapiens
17 DHFRL1  
Proximity Label-MS Homo sapiens
18 KRAS 3845
Proximity Label-MS Homo sapiens
19 RHOB 388
Proximity Label-MS Homo sapiens
20 HAX1  
Proximity Label-MS Homo sapiens
21 CCDC67  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CCT8L2  
Affinity Capture-MS Homo sapiens
23 C17orf59 54785
Proximity Label-MS Homo sapiens
24 ACBD5 91452
Proximity Label-MS Homo sapiens
25 HYPM  
Affinity Capture-MS Homo sapiens
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