Gene description for DPH2
Gene name DPH2 homolog (S. cerevisiae)
Gene symbol DPH2
Other names/aliases DPH2L2
Species Homo sapiens
 Database cross references - DPH2
ExoCarta ExoCarta_1802
Vesiclepedia VP_1802
Entrez Gene 1802
HGNC 3004
MIM 603456
UniProt Q9BQC3  
 DPH2 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
 Gene ontology annotations for DPH2
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 ISS
    2-(3-amino-3-carboxypropyl)histidine synthase activity GO:0090560 EXP
    2-(3-amino-3-carboxypropyl)histidine synthase activity GO:0090560 ISS
Biological Process
    protein histidyl modification to diphthamide GO:0017183 IBA
    protein histidyl modification to diphthamide GO:0017183 IEA
    protein histidyl modification to diphthamide GO:0017183 IMP
    protein histidyl modification to diphthamide GO:0017183 ISS
    protein histidyl modification to diphthamide GO:0017183 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    2-(3-amino-3-carboxypropyl)histidine synthase complex GO:0120513 ISS
 Experiment description of studies that identified DPH2 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for DPH2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
2 DPH3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 DPYSL2 1808
Co-fractionation Homo sapiens
4 FASN 2194
Positive Genetic Homo sapiens
5 CRYGA 1418
Two-hybrid Homo sapiens
6 COLEC12 81035
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 IL17A  
Two-hybrid Homo sapiens
8 DPH1  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
9 HSPA8 3312
Affinity Capture-MS Homo sapiens
10 NEK6 10783
Two-hybrid Homo sapiens
11 BAG2 9532
Affinity Capture-MS Homo sapiens
12 RIT1 6016
Negative Genetic Homo sapiens
13 HSPBP1 23640
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 FARS2 10667
Two-hybrid Homo sapiens
15 EEF2 1938
Co-fractionation Homo sapiens
16 CAP1 10487
Co-fractionation Homo sapiens
17 CDA 978
Co-fractionation Homo sapiens
18 TSG101 7251
Co-fractionation Homo sapiens
19 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
20 RPRD1A  
Co-fractionation Homo sapiens
21 FAM219B 57184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ZC2HC1B  
Affinity Capture-MS Homo sapiens
23 SULT4A1  
Affinity Capture-MS Homo sapiens
24 RBFOX2 23543
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 GNA11 2767
Co-fractionation Homo sapiens
26 DNAJC24  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
27 IL17B 27190
Affinity Capture-MS Homo sapiens
28 BAG1 573
Affinity Capture-MS Homo sapiens
29 RTCB 51493
Co-fractionation Homo sapiens
30 FDPS 2224
Co-fractionation Homo sapiens
31 WIPI2 26100
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 STIM2 57620
Affinity Capture-MS Homo sapiens
33 NHP2L1 4809
Co-fractionation Homo sapiens
34 HSPA1A 3303
Affinity Capture-MS Homo sapiens
35 MID1IP1  
Affinity Capture-MS Homo sapiens
36 KRAS 3845
Negative Genetic Homo sapiens
37 MAGOH 4116
Two-hybrid Homo sapiens
38 DDX58 23586
Co-fractionation Homo sapiens
39 RGS20 8601
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ICAM4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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