Gene description for CDA
Gene name cytidine deaminase
Gene symbol CDA
Other names/aliases CDD
Species Homo sapiens
 Database cross references - CDA
ExoCarta ExoCarta_978
Vesiclepedia VP_978
Entrez Gene 978
HGNC 1712
MIM 123920
UniProt P32320  
 CDA identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for CDA
Molecular Function
    nucleoside binding GO:0001882 IDA
    cytidine deaminase activity GO:0004126 IBA
    cytidine deaminase activity GO:0004126 IDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
Biological Process
    cell surface receptor signaling pathway GO:0007166 NAS
    pyrimidine-containing compound salvage GO:0008655 NAS
    cytidine deamination GO:0009972 IBA
    cytidine deamination GO:0009972 IDA
    cytosine metabolic process GO:0019858 TAS
    negative regulation of cell growth GO:0030308 IDA
    UMP salvage GO:0044206 IEA
    negative regulation of nucleotide metabolic process GO:0045980 IDA
    response to cycloheximide GO:0046898 IEA
    cellular response to external biotic stimulus GO:0071217 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 NAS
    cytosol GO:0005829 TAS
    secretory granule lumen GO:0034774 TAS
    tertiary granule lumen GO:1904724 TAS
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified CDA in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CDA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DPH2 1802
Co-fractionation Homo sapiens
2 SDCBP 6386
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 SMARCA2 6595
Affinity Capture-MS Homo sapiens
4 ADAT3 113179
Two-hybrid Homo sapiens
5 WARS 7453
Co-fractionation Homo sapiens
6 CPNE3 8895
Co-fractionation Homo sapiens
7 RNF181  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 XPO1 7514
Co-fractionation Homo sapiens
9 CDA 978
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 ZBTB24  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 PFKP 5214
Co-fractionation Homo sapiens
12 INCA1  
Two-hybrid Homo sapiens
13 GAPDHS 26330
Affinity Capture-MS Homo sapiens
14 GPX4 2879
Affinity Capture-MS Homo sapiens
15 APBA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TTC38 55020
Co-fractionation Homo sapiens
17 FOXN1  
Two-hybrid Homo sapiens
18 DDIT4L  
Two-hybrid Homo sapiens
19 CPNE1 8904
Co-fractionation Homo sapiens
20 PTGER3  
Two-hybrid Homo sapiens
21 MTMR9 66036
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 TRIM25 7706
Co-fractionation Homo sapiens
23 PSMA1 5682
Two-hybrid Homo sapiens
24 G6PD 2539
Co-fractionation Homo sapiens
25 LIN7A 8825
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 KRTAP8-1  
Two-hybrid Homo sapiens
27 PLEKHB2 55041
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 ZMYM6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 C20orf195  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 TIMM8A 1678
Co-fractionation Homo sapiens
31 WDYHV1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 DPYSL2 1808
Co-fractionation Homo sapiens
33 MCM2 4171
Affinity Capture-MS Homo sapiens
34 GORASP2 26003
Two-hybrid Homo sapiens
35 GBE1 2632
Co-fractionation Homo sapiens
36 ARRDC3 57561
Two-hybrid Homo sapiens
37 EVPL 2125
Co-fractionation Homo sapiens
38 MPP6 51678
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 SH3KBP1 30011
Co-fractionation Homo sapiens
40 MPP2 4355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 RC3H1 149041
Co-fractionation Homo sapiens
42 LYPLA1 10434
Co-fractionation Homo sapiens
43 CLIC4 25932
Co-fractionation Homo sapiens
44 WIBG 84305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 KRTAP6-1  
Two-hybrid Homo sapiens
46 LNX1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
47 CDKN2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 LACTB 114294
Affinity Capture-MS Homo sapiens
49 MTMR6 9107
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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