Experiment description of studies that identified MPP6 in sEVs

1 Experiment ID 79 MISEV standards ✘ Biophysical techniques ✔ CD81|MHCII Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20458337     Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W." Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting

2 Experiment ID 80 MISEV standards ✘ Biophysical techniques ✔ CD81|MHCII Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20458337     Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W." Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting

3 Experiment ID 81 MISEV standards ✘ Biophysical techniques ✔ CD81|MHCII Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20458337     Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W." Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting

4 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 260 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1|CD81 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name 1205Lu Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

9 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

10 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for MPP6

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HNRNPH1 3187 Affinity Capture-RNA Homo sapiens 2 FTL 2512 Two-hybrid Homo sapiens 3 EXOSC10 5394 Two-hybrid Homo sapiens 4 EIF1AD 84285 Affinity Capture-MS Homo sapiens 5 ZRANB1 54764 Affinity Capture-MS Homo sapiens 6 SSTR3   Affinity Capture-MS Homo sapiens 7 EIF3G 8666 Two-hybrid Homo sapiens 8 OGT 8473 Reconstituted Complex Homo sapiens 9 Lin7c 22343 Affinity Capture-MS Mus musculus 10 NAT9   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 TRPS1   Affinity Capture-MS Homo sapiens 12 DNM2 1785 Two-hybrid Homo sapiens 13 LIN7B   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 14 CDA 978 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 NF2 4771 Affinity Capture-MS Homo sapiens 16 NSUN2 54888 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 EGLN3   Affinity Capture-MS Homo sapiens 18 THOP1 7064 Two-hybrid Homo sapiens 19 CYP2S1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 DYNLRB1 83658 Two-hybrid Homo sapiens 21 TRIO 7204 Affinity Capture-MS Homo sapiens 22 NUMA1 4926 Affinity Capture-MS Homo sapiens 23 ARHGAP18 93663 Two-hybrid Homo sapiens 24 LIPT1   Affinity Capture-MS Homo sapiens 25 ACADVL 37 Co-fractionation Homo sapiens 26 MAGEA9   Affinity Capture-MS Homo sapiens 27 C11orf52 91894 Proximity Label-MS Homo sapiens 28 THRSP   Affinity Capture-MS Homo sapiens 29 OPTN 10133 Affinity Capture-MS Homo sapiens 30 LYN 4067 Proximity Label-MS Homo sapiens 31 SNX9 51429 Two-hybrid Homo sapiens 32 LIN7A 8825 Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens 33 DEFB136   Affinity Capture-MS Homo sapiens 34 FAM19A3   Affinity Capture-MS Homo sapiens 35 RPS20 6224 Two-hybrid Homo sapiens 36 SRP9 6726 Affinity Capture-MS Homo sapiens 37 KNSTRN   Two-hybrid Homo sapiens 38 SLC20A1 6574 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 39 MT2P1   Two-hybrid Homo sapiens 40 NTRK1 4914 Affinity Capture-MS Homo sapiens 41 LIN7C 55327 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 42 CENPM   Affinity Capture-MS Homo sapiens 43 C1orf174   Affinity Capture-MS Homo sapiens 44 NFKB1 4790 Reconstituted Complex Homo sapiens 45 EPHA2 1969 Proximity Label-MS Homo sapiens 46 SKIV2L2 23517 Two-hybrid Homo sapiens 47 CHMP4B 128866 Affinity Capture-MS Homo sapiens 48 KHDRBS1 10657 Protein-peptide Homo sapiens 49 KRAS 3845 Proximity Label-MS Homo sapiens 50 MPP2 4355 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 51 FAM188A 80013 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 52 DMD 1756 Affinity Capture-MS Homo sapiens 53 SMARCA4 6597 Two-hybrid Homo sapiens 54 KCNJ12 3768 Affinity Capture-MS Homo sapiens 55 NDN 4692 Reconstituted Complex Homo sapiens 56 AATF   Two-hybrid Homo sapiens 57 EP300 2033 Affinity Capture-MS Homo sapiens 58 P2RY12 64805 Affinity Capture-MS Homo sapiens 59 PARN   Two-hybrid Homo sapiens View the network

Pathways in which MPP6 is involved

No pathways found