Gene description for KCNJ12
Gene name potassium channel, inwardly rectifying subfamily J, member 12
Gene symbol KCNJ12
Other names/aliases IRK-2
IRK2
KCNJN1
Kir2.2
Kir2.2v
hIRK
hIRK1
hkir2.2x
kcnj12x
Species Homo sapiens
 Database cross references - KCNJ12
ExoCarta ExoCarta_3768
Vesiclepedia VP_3768
Entrez Gene 3768
HGNC 6258
MIM 602323
UniProt Q14500  
 KCNJ12 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for KCNJ12
Molecular Function
    inward rectifier potassium channel activity GO:0005242 IBA
    inward rectifier potassium channel activity GO:0005242 IDA
    protein binding GO:0005515 IPI
Biological Process
    potassium ion transport GO:0006813 IDA
    muscle contraction GO:0006936 TAS
    regulation of heart contraction GO:0008016 TAS
    regulation of monoatomic ion transmembrane transport GO:0034765 IBA
    protein homotetramerization GO:0051289 IDA
    potassium ion import across plasma membrane GO:1990573 IBA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IDA
    T-tubule GO:0030315 ISS
    monoatomic ion channel complex GO:0034702 IEA
 Experiment description of studies that identified KCNJ12 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for KCNJ12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DLG2 1740
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 LIN7A 8825
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 SNTB2 6645
Affinity Capture-MS Homo sapiens
4 DLG1 1739
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 APBA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 KCNMB3  
Affinity Capture-MS Homo sapiens
7 ABLIM1 3983
Affinity Capture-MS Homo sapiens
8 DTNA  
Affinity Capture-MS Homo sapiens
9 KCNJ10  
Affinity Capture-MS Homo sapiens
10 DMD 1756
Affinity Capture-MS Homo sapiens
11 SNTA1 6640
Affinity Capture-MS Homo sapiens
12 MPP6 51678
Affinity Capture-MS Homo sapiens
13 EMD 2010
Two-hybrid Homo sapiens
14 SNTB1 6641
Affinity Capture-MS Homo sapiens
15 LIN7B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 DLG4  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 LIN7C 55327
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
18 DLG3 1741
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
19 CASK 8573
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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