Gene description for ELF3
Gene name E74-like factor 3 (ets domain transcription factor, epithelial-specific )
Gene symbol ELF3
Other names/aliases EPR-1
ERT
ESE-1
ESX
Species Homo sapiens
 Database cross references - ELF3
ExoCarta ExoCarta_1999
Vesiclepedia VP_1999
Entrez Gene 1999
HGNC 3318
MIM 602191
UniProt P78545  
 ELF3 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ELF3
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IEA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA-binding transcription factor activity GO:0003700 TAS
    protein binding GO:0005515 IPI
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    blastocyst development GO:0001824 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    inflammatory response GO:0006954 IEP
    cell differentiation GO:0030154 IBA
    cell differentiation GO:0030154 IEA
    extracellular matrix organization GO:0030198 IEA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    mammary gland involution GO:0060056 ISS
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified ELF3 in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ELF3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RSL1D1 26156
Affinity Capture-MS Homo sapiens
2 RELA 5970
Affinity Capture-Western Homo sapiens
3 CALR 811
Affinity Capture-MS Homo sapiens
4 ISG15 9636
Affinity Capture-MS Homo sapiens
5 BTRC 8945
Affinity Capture-Western Homo sapiens
6 ERBB2 2064
Synthetic Rescue Homo sapiens
7 QARS 5859
Affinity Capture-MS Homo sapiens
8 RPL6 6128
Affinity Capture-MS Homo sapiens
9 PAK1 5058
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
10 DPF1  
Proximity Label-MS Homo sapiens
11 UBE2M 9040
Affinity Capture-MS Homo sapiens
12 DDX3X 1654
Affinity Capture-MS Homo sapiens
13 TRIM24  
Proximity Label-MS Homo sapiens
14 PBRM1 55193
Proximity Label-MS Homo sapiens
15 FLOT2 2319
Affinity Capture-MS Homo sapiens
16 GRPEL1 80273
Proximity Label-MS Homo sapiens
17 KRT82 3888
Affinity Capture-MS Homo sapiens
18 GADD45GIP1  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
19 HNRNPUL1 11100
Affinity Capture-MS Homo sapiens
20 TARS 6897
Affinity Capture-MS Homo sapiens
21 CORO1C 23603
Affinity Capture-MS Homo sapiens
22 TBP  
Reconstituted Complex Homo sapiens
23 ELF3 1999
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 NOP2 4839
Affinity Capture-MS Homo sapiens
25 EBNA1BP2 10969
Affinity Capture-MS Homo sapiens
26 TPM1 7168
Affinity Capture-MS Homo sapiens
27 CBR3 874
Affinity Capture-MS Homo sapiens
28 TAF9 6880
Reconstituted Complex Homo sapiens
29 MYO6 4646
Affinity Capture-MS Homo sapiens
30 MYC  
Biochemical Activity Homo sapiens
31 XRCC6 2547
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
32 JAK1 3716
Affinity Capture-MS Homo sapiens
33 EEF1D 1936
Affinity Capture-MS Homo sapiens
34 CECR2  
Affinity Capture-MS Homo sapiens
35 NFKB1 4790
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
36 MED23 9439
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
37 P4HB 5034
Affinity Capture-MS Homo sapiens
38 GANAB 23193
Affinity Capture-MS Homo sapiens
39 AR 367
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
40 IARS 3376
Affinity Capture-MS Homo sapiens
41 EHF  
Affinity Capture-MS Homo sapiens
42 VPS72  
Proximity Label-MS Homo sapiens
43 KRT31 3881
Affinity Capture-MS Homo sapiens
44 Jun  
Biochemical Activity Rattus norvegicus
45 GNL3 26354
Affinity Capture-MS Homo sapiens
46 XRCC5 7520
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
47 EP300 2033
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
48 ATIC 471
Affinity Capture-MS Homo sapiens
49 AIMP1 9255
Affinity Capture-MS Homo sapiens
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