Gene description for Arhgap1
Gene name Rho GTPase activating protein 1
Gene symbol Arhgap1
Other names/aliases B230365D05Rik
C76222
Cdc42GAP
p50rhoGAP
Species Mus musculus
 Database cross references - Arhgap1
ExoCarta ExoCarta_228359
Vesiclepedia VP_228359
Entrez Gene 228359
UniProt Q5FWK3  
 Arhgap1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Arhgap1
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IDA
    GTPase activator activity GO:0005096 ISO
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    small GTPase binding GO:0031267 ISO
Biological Process
    small GTPase-mediated signal transduction GO:0007264 IBA
    small GTPase-mediated signal transduction GO:0007264 ISO
    endosomal transport GO:0016197 ISO
    transferrin transport GO:0033572 ISO
    regulation of GTPase activity GO:0043087 IDA
    negative regulation of endocytic recycling GO:2001136 IBA
    negative regulation of endocytic recycling GO:2001136 ISO
Subcellular Localization
    ruffle GO:0001726 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 IDA
    endosome membrane GO:0010008 ISO
    cell leading edge GO:0031252 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    sorting endosome GO:0097443 ISO
 Experiment description of studies that identified Arhgap1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Arhgap1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNBP 7555
Affinity Capture-MS Homo sapiens
2 WDR82 80335
Affinity Capture-MS Homo sapiens
3 NUDT21 11051
Affinity Capture-MS Homo sapiens
4 GLUD1 2746
Affinity Capture-MS Homo sapiens
5 PSPC1 55269
Affinity Capture-MS Homo sapiens
6 TACO1  
Affinity Capture-MS Homo sapiens
7 ARHGEF19  
Affinity Capture-MS Homo sapiens
8 MAZ 4150
Affinity Capture-MS Homo sapiens
9 PRPF4B 8899
Affinity Capture-MS Homo sapiens
10 Rhoa 11848
Reconstituted Complex Mus musculus
11 HMGB1 3146
Affinity Capture-MS Homo sapiens
12 PPIL4  
Affinity Capture-MS Homo sapiens
13 PRDX2 7001
Affinity Capture-MS Homo sapiens
14 LMNB2 84823
Affinity Capture-MS Homo sapiens
15 C1orf35  
Affinity Capture-MS Homo sapiens
16 CA2 760
Affinity Capture-MS Homo sapiens
17 ZNF207 7756
Affinity Capture-MS Homo sapiens
18 PYCR2 29920
Affinity Capture-MS Homo sapiens
19 BOLA2 552900
Affinity Capture-MS Homo sapiens
20 STOML2 30968
Affinity Capture-MS Homo sapiens
21 CDC42 998
FRET Homo sapiens
22 Tmem173  
Proximity Label-MS Mus musculus
23 POTEE 445582
Affinity Capture-MS Homo sapiens
24 DDX42 11325
Affinity Capture-MS Homo sapiens
25 CSNK1D 1453
Affinity Capture-MS Homo sapiens
26 Cdc42 12540
Reconstituted Complex Mus musculus
27 PHF6  
Affinity Capture-MS Homo sapiens
28 CDK9 1025
Affinity Capture-MS Homo sapiens
29 LRPAP1 4043
Affinity Capture-MS Homo sapiens
30 FIP1L1 81608
Affinity Capture-MS Homo sapiens
31 LUC7L2 51631
Affinity Capture-MS Homo sapiens
32 Eed  
Affinity Capture-MS Mus musculus
33 Drg1  
Co-fractionation Mus musculus
34 CKAP4 10970
Affinity Capture-MS Homo sapiens
35 RHOA 387
FRET Homo sapiens
36 LMNA 4000
Affinity Capture-MS Homo sapiens
37 Rac1 19353
Biochemical Activity Mus musculus
Reconstituted Complex Mus musculus
38 Hdlbp 110611
Co-fractionation Mus musculus
39 CCNT1  
Affinity Capture-MS Homo sapiens
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