Gene description for Cdc42
Gene name cell division cycle 42
Gene symbol Cdc42
Other names/aliases AI747189
AU018915
Species Mus musculus
 Database cross references - Cdc42
ExoCarta ExoCarta_12540
Vesiclepedia VP_12540
Entrez Gene 12540
UniProt P60766  
 Cdc42 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cdc42
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 IGI
    GTPase activity GO:0003924 ISA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 ISO
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 IPI
    protein kinase binding GO:0019901 ISO
    GTP-dependent protein binding GO:0030742 IPI
    mitogen-activated protein kinase kinase kinase binding GO:0031435 IEA
    mitogen-activated protein kinase kinase kinase binding GO:0031435 ISO
    thioesterase binding GO:0031996 IEA
    thioesterase binding GO:0031996 ISO
    GBD domain binding GO:0032427 IEA
    GBD domain binding GO:0032427 ISO
    apolipoprotein A-I receptor binding GO:0034191 IEA
    apolipoprotein A-I receptor binding GO:0034191 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    ubiquitin protein ligase activity GO:0061630 IEA
    ubiquitin protein ligase activity GO:0061630 ISO
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    sprouting angiogenesis GO:0002040 IEA
    sprouting angiogenesis GO:0002040 ISO
    heart process GO:0003015 IMP
    cardiac conduction system development GO:0003161 IGI
    cardiac neural crest cell migration involved in outflow tract morphogenesis GO:0003253 IMP
    protein phosphorylation GO:0006468 IMP
    endocytosis GO:0006897 IBA
    endocytosis GO:0006897 IMP
    phagocytosis, engulfment GO:0006911 ISO
    phagocytosis, engulfment GO:0006911 ISS
    actin filament organization GO:0007015 IBA
    actin filament organization GO:0007015 ISO
    actin filament organization GO:0007015 ISS
    Golgi organization GO:0007030 IEA
    Golgi organization GO:0007030 ISO
    regulation of mitotic nuclear division GO:0007088 IMP
    nuclear migration GO:0007097 IMP
    establishment or maintenance of cell polarity GO:0007163 TAS
    integrin-mediated signaling pathway GO:0007229 IEA
    integrin-mediated signaling pathway GO:0007229 ISO
    Rho protein signal transduction GO:0007266 TAS
    protein localization GO:0008104 IMP
    regulation of lamellipodium assembly GO:0010591 ISO
    positive regulation of lamellipodium assembly GO:0010592 IEA
    positive regulation of lamellipodium assembly GO:0010592 ISO
    endosomal transport GO:0016197 TAS
    establishment of cell polarity GO:0030010 IBA
    actin cytoskeleton organization GO:0030036 ISO
    positive regulation of cell growth GO:0030307 IEA
    positive regulation of cell growth GO:0030307 ISO
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of pseudopodium assembly GO:0031274 IEA
    positive regulation of pseudopodium assembly GO:0031274 ISO
    negative regulation of protein-containing complex assembly GO:0031333 IEA
    negative regulation of protein-containing complex assembly GO:0031333 ISO
    positive regulation of cytokinesis GO:0032467 ISO
    positive regulation of cytokinesis GO:0032467 ISS
    Cdc42 protein signal transduction GO:0032488 IBA
    Cdc42 protein signal transduction GO:0032488 ISO
    regulation of actin cytoskeleton organization GO:0032956 ISO
    cell junction assembly GO:0034329 ISO
    cell junction assembly GO:0034329 ISS
    adherens junction organization GO:0034332 IMP
    embryonic heart tube development GO:0035050 IMP
    establishment or maintenance of apical/basal cell polarity GO:0035088 IMP
    dendritic cell migration GO:0036336 IGI
    neuropilin signaling pathway GO:0038189 IEA
    neuropilin signaling pathway GO:0038189 ISO
    regulation of protein binding GO:0043393 IDA
    positive regulation of MAPK cascade GO:0043410 IDA
    positive regulation of neuron apoptotic process GO:0043525 IEA
    positive regulation of neuron apoptotic process GO:0043525 ISO
    modulation by host of viral process GO:0044788 IEA
    modulation by host of viral process GO:0044788 ISO
    establishment of epithelial cell apical/basal polarity GO:0045198 ISO
    establishment of epithelial cell apical/basal polarity GO:0045198 ISS
    positive regulation of DNA replication GO:0045740 IEA
    positive regulation of DNA replication GO:0045740 ISO
    positive regulation of JNK cascade GO:0046330 IEA
    positive regulation of JNK cascade GO:0046330 ISO
    filopodium assembly GO:0046847 IDA
    positive regulation of pinocytosis GO:0048549 IMP
    neuron fate determination GO:0048664 IMP
    regulation of filopodium assembly GO:0051489 IBA
    regulation of filopodium assembly GO:0051489 ISO
    regulation of filopodium assembly GO:0051489 ISS
    positive regulation of filopodium assembly GO:0051491 IMP
    positive regulation of filopodium assembly GO:0051491 ISO
    regulation of stress fiber assembly GO:0051492 ISO
    positive regulation of stress fiber assembly GO:0051496 IEA
    positive regulation of stress fiber assembly GO:0051496 ISO
    nucleus localization GO:0051647 IMP
    establishment of localization in cell GO:0051649 IMP
    establishment of Golgi localization GO:0051683 IEA
    establishment of Golgi localization GO:0051683 ISO
    positive regulation of synapse structural plasticity GO:0051835 IEA
    positive regulation of synapse structural plasticity GO:0051835 ISO
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IMP
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 ISO
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 ISS
    heart contraction GO:0060047 IGI
    positive regulation of epithelial cell proliferation involved in lung morphogenesis GO:0060501 IMP
    submandibular salivary gland formation GO:0060661 IEA
    dendritic spine morphogenesis GO:0060997 IMP
    cellular response to type II interferon GO:0071346 IDA
    organelle transport along microtubule GO:0072384 IEA
    organelle transport along microtubule GO:0072384 ISO
    endothelin receptor signaling pathway involved in heart process GO:0086101 IMP
    actin filament branching GO:0090135 IEA
    actin filament branching GO:0090135 ISO
    positive regulation of intracellular protein transport GO:0090316 IEA
    positive regulation of intracellular protein transport GO:0090316 ISO
    cell-cell adhesion GO:0098609 TAS
    regulation of modification of postsynaptic structure GO:0099159 IEA
    regulation of modification of postsynaptic structure GO:0099159 ISO
    regulation of postsynapse organization GO:0099175 IDA
    regulation of postsynapse organization GO:0099175 IMP
    modification of synaptic structure GO:0099563 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 ISO
    storage vacuole GO:0000322 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    Golgi transport complex GO:0017119 IEA
    Golgi transport complex GO:0017119 ISO
    secretory granule GO:0030141 IEA
    secretory granule GO:0030141 ISO
    filopodium GO:0030175 IEA
    filopodium GO:0030175 ISO
    dendrite GO:0030425 IEA
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    cell leading edge GO:0031252 IDA
    leading edge membrane GO:0031256 IDA
    lamellipodium membrane GO:0031258 IEA
    protein-containing complex GO:0032991 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    cell projection GO:0042995 IDA
    neuron projection GO:0043005 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    dendritic spine GO:0043197 IBA
    dendritic spine GO:0043197 ISO
    myelin sheath GO:0043209 HDA
    apical part of cell GO:0045177 IDA
    phagocytic vesicle GO:0045335 IDA
    spindle midzone GO:0051233 ISO
    spindle midzone GO:0051233 ISS
    cell periphery GO:0071944 IDA
    mitotic spindle GO:0072686 ISO
    mitotic spindle GO:0072686 ISS
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 IMP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Cdc42 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cdc42
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ptpn2  
Co-fractionation Mus musculus
2 SECISBP2  
Two-hybrid Homo sapiens
3 Arhgap33  
Affinity Capture-Western Mus musculus
4 Sarnp 66118
Co-fractionation Mus musculus
5 Stau1  
Co-fractionation Mus musculus
6 Cit 12704
Affinity Capture-Western Mus musculus
7 Arhgap1 228359
Reconstituted Complex Mus musculus
8 DENND1A 57706
Two-hybrid Homo sapiens
9 Homer2  
Affinity Capture-Western Mus musculus
10 NPHS1 4868
Phenotypic Suppression Homo sapiens
11 Lrrk2  
Co-localization Mus musculus
12 Arhgap32  
Biochemical Activity Mus musculus
13 Rock2  
Co-fractionation Mus musculus
14 Agap2  
Affinity Capture-MS Mus musculus
15 Dock9  
Affinity Capture-Western Mus musculus
16 Unk  
Affinity Capture-RNA Mus musculus
17 Sstr3  
Proximity Label-MS Mus musculus
18 Rac1 19353
Co-fractionation Mus musculus
19 Dock11  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
20 Pak1  
Affinity Capture-Western Mus musculus
21 Fancd2  
Affinity Capture-MS Mus musculus
22 Dock10 210293
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Cdc42 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
CD28 co-stimulation IEA Reactome
CD28 dependent Vav1 pathway IEA Reactome
CDC42 GTPase cycle IEA Reactome
Costimulation by the CD28 family IEA Reactome
DCC mediated attractive signaling IEA Reactome
Developmental Biology IEA Reactome
EGFR downregulation IEA Reactome
EPH-Ephrin signaling IEA Reactome
EPHB-mediated forward signaling IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
G beta:gamma signalling through CDC42 IEA Reactome
G-protein beta:gamma signalling IEA Reactome
GPCR downstream signalling IEA Reactome
GPVI-mediated activation cascade IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK6/MAPK4 signaling IEA Reactome
Myogenesis IEA Reactome
Nervous system development IEA Reactome
Netrin-1 signaling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
RAC1 GTPase cycle IEA Reactome
RAC2 GTPase cycle IEA Reactome
RAC3 GTPase cycle IEA Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
RHO GTPase cycle IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
RHO GTPases activate KTN1 IEA Reactome
RHO GTPases activate PAKs IEA Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
RHOG GTPase cycle IEA Reactome
RHOQ GTPase cycle IEA Reactome
RHOU GTPase cycle IEA Reactome
RHOV GTPase cycle IEA Reactome
Signal Transduction IEA Reactome
Signaling by EGFR IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by VEGF IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome





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