Gene description for Cit
Gene name citron
Gene symbol Cit
Other names/aliases C030025P15Rik
CRIK
CRIK-SK
Cit-k
Species Mus musculus
 Database cross references - Cit
ExoCarta ExoCarta_12704
Vesiclepedia VP_12704
Entrez Gene 12704
UniProt P49025  
 Cit identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cit
Molecular Function
    transcription coactivator binding GO:0001223 ISO
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    SH3 domain binding GO:0017124 IEA
    protein kinase binding GO:0019901 ISO
    PDZ domain binding GO:0030165 ISO
    protein serine/threonine kinase inhibitor activity GO:0030291 ISO
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    scaffold protein binding GO:0097110 ISO
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    mitotic sister chromatid segregation GO:0000070 IMP
    G2/M transition of mitotic cell cycle GO:0000086 ISO
    mitotic cell cycle GO:0000278 IDA
    mitotic cytokinesis GO:0000281 ISO
    mitotic cytokinesis GO:0000281 ISS
    cytokinesis GO:0000910 IMP
    chromatin remodeling GO:0006338 IEA
    Golgi organization GO:0007030 ISO
    metaphase/anaphase transition of mitotic cell cycle GO:0007091 IMP
    gamma-aminobutyric acid signaling pathway GO:0007214 ISO
    spermatogenesis GO:0007283 IMP
    regulation of actin polymerization or depolymerization GO:0008064 ISO
    dendrite development GO:0016358 IMP
    positive regulation of cytokinesis GO:0032467 ISO
    regulation of actin cytoskeleton organization GO:0032956 IBA
    negative regulation of hippo signaling GO:0035331 ISO
    negative regulation of neuron differentiation GO:0045665 ISO
    generation of neurons GO:0048699 IDA
    negative regulation of dendrite morphogenesis GO:0050774 IDA
    neuron apoptotic process GO:0051402 ISO
    neuron apoptotic process GO:0051402 ISS
    postsynaptic density protein 95 clustering GO:0097119 ISO
    postsynaptic cytoskeleton organization GO:0099188 ISO
Subcellular Localization
    ruffle GO:0001726 IDA
    cytoplasm GO:0005737 IDA
    vacuole GO:0005773 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    actin cytoskeleton GO:0015629 IDA
    midbody GO:0030496 ISO
    Golgi cisterna GO:0031985 ISO
    cleavage furrow GO:0032154 ISO
    neuronal cell body GO:0043025 ISO
    GABA-ergic synapse GO:0098982 ISO
    postsynaptic density, intracellular component GO:0099092 ISO
    glutamatergic postsynaptic density GO:0099573 ISO
 Experiment description of studies that identified Cit in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cit
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tnik  
Affinity Capture-MS Mus musculus
2 PRKAA1 5562
Affinity Capture-MS Homo sapiens
3 Pde4dip  
Affinity Capture-MS Mus musculus
4 CEP131 22994
Affinity Capture-MS Homo sapiens
5 KIF14 9928
Affinity Capture-MS Homo sapiens
6 Kalrn  
Affinity Capture-MS Mus musculus
7 KIAA0753  
Affinity Capture-MS Homo sapiens
8 Ttc3  
Affinity Capture-Western Mus musculus
9 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
10 LATS2 26524
Affinity Capture-Western Homo sapiens
11 Kctd13  
Affinity Capture-MS Mus musculus
12 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
13 TTC3 7267
Two-hybrid Homo sapiens
14 Hcn1  
Affinity Capture-MS Mus musculus
15 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
16 GTPBP1 9567
Affinity Capture-MS Homo sapiens
17 Rnd3  
Co-fractionation Mus musculus
18 LATS1  
Affinity Capture-Western Homo sapiens
19 Grin2b  
Affinity Capture-Western Mus musculus
20 Lats2 50523
Co-localization Mus musculus
21 Cdc42 12540
Affinity Capture-Western Mus musculus
22 Rhoa 11848
Affinity Capture-Western Mus musculus
23 Grin1  
Affinity Capture-Western Mus musculus
24 Rhob 11852
Co-fractionation Mus musculus
25 Yap1  
Affinity Capture-Western Mus musculus
Co-localization Mus musculus
Affinity Capture-Western Mus musculus
26 PCM1 5108
Affinity Capture-MS Homo sapiens
27 CEP72  
Affinity Capture-MS Homo sapiens
28 Rac1 19353
Affinity Capture-Western Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here