Gene description for DLGAP4
Gene name discs, large (Drosophila) homolog-associated protein 4
Gene symbol DLGAP4
Other names/aliases DAP-4
DAP4
DLP4
SAPAP-4
SAPAP4
Species Homo sapiens
 Database cross references - DLGAP4
ExoCarta ExoCarta_22839
Vesiclepedia VP_22839
Entrez Gene 22839
HGNC 24476
MIM 616191
UniProt Q9Y2H0  
 DLGAP4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DLGAP4
Molecular Function
    protein binding GO:0005515 IPI
    molecular adaptor activity GO:0060090 IBA
Biological Process
    signaling GO:0023052 IEA
    modulation of chemical synaptic transmission GO:0050804 IBA
    regulation of synapse maturation GO:0090128 IEA
    modification of postsynaptic structure GO:0099010 IEA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    neuromuscular junction GO:0031594 IEA
    glutamatergic synapse GO:0098978 IBA
    cholinergic synapse GO:0098981 IEA
    postsynaptic specialization GO:0099572 IBA
 Experiment description of studies that identified DLGAP4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DLGAP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNX1 6642
Affinity Capture-MS Homo sapiens
2 PLS3 5358
Co-fractionation Homo sapiens
3 SHANK3 85358
Two-hybrid Homo sapiens
4 OCLN 100506658
Proximity Label-MS Homo sapiens
5 DVL2 1856
Affinity Capture-MS Homo sapiens
6 DLGAP2  
Affinity Capture-MS Homo sapiens
7 HSPA8 3312
Affinity Capture-MS Homo sapiens
8 DLG3 1741
Affinity Capture-MS Homo sapiens
9 CASK 8573
Affinity Capture-MS Homo sapiens
10 SHANK2 22941
Two-hybrid Homo sapiens
11 PPP1R1B  
Two-hybrid Homo sapiens
12 Nek2  
Affinity Capture-MS Mus musculus
13 DLG1 1739
Affinity Capture-MS Homo sapiens
14 B4galt1  
Affinity Capture-MS Mus musculus
15 ALDH16A1 126133
Affinity Capture-MS Homo sapiens
16 XRCC2  
Affinity Capture-MS Homo sapiens
17 UBC 7316
Two-hybrid Homo sapiens
18 SORBS2  
Reconstituted Complex Homo sapiens
19 Ncapg2  
Affinity Capture-MS Mus musculus
20 DLG4  
Two-hybrid Homo sapiens
21 DERA 51071
Affinity Capture-MS Homo sapiens
22 Ppp6c 67857
Affinity Capture-MS Mus musculus
23 PRSS42  
Affinity Capture-MS Homo sapiens
24 NXF1 10482
Affinity Capture-RNA Homo sapiens
25 LIN7A 8825
Affinity Capture-MS Homo sapiens
26 MAGI3 260425
Affinity Capture-MS Homo sapiens
27 PRDM16 63976
Affinity Capture-MS Homo sapiens
28 ALYREF 10189
Affinity Capture-MS Homo sapiens
29 PARD6B 84612
Affinity Capture-MS Homo sapiens
30 PAK4 10298
Co-fractionation Homo sapiens
31 LIN7C 55327
Affinity Capture-MS Homo sapiens
32 HSPB8 26353
Affinity Capture-MS Homo sapiens
33 MOV10 4343
Affinity Capture-RNA Homo sapiens
34 EPHA2 1969
Proximity Label-MS Homo sapiens
35 KRAS 3845
Proximity Label-MS Homo sapiens
36 RHOB 388
Proximity Label-MS Homo sapiens
37 Pcm1  
Affinity Capture-MS Mus musculus
38 MPP7 143098
Affinity Capture-MS Homo sapiens
39 Pdlim5 56376
Affinity Capture-MS Mus musculus
40 ENAH 55740
Affinity Capture-MS Homo sapiens
41 SH3GL2  
Two-hybrid Homo sapiens
42 BIN1 274
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
43 VASP 7408
Affinity Capture-MS Homo sapiens
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