Gene description for SPG20
Gene name spastic paraplegia 20 (Troyer syndrome)
Gene symbol SPG20
Other names/aliases SPARTIN
TAHCCP1
Species Homo sapiens
 Database cross references - SPG20
ExoCarta ExoCarta_23111
Vesiclepedia VP_23111
Entrez Gene 23111
HGNC 18514
MIM 607111
UniProt Q8N0X7  
 SPG20 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Melanoma cells 25950383    
 Gene ontology annotations for SPG20
Molecular Function
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
Biological Process
    lipid transport GO:0006869 IDA
    lipid catabolic process GO:0016042 IEA
    BMP signaling pathway GO:0030509 IEA
    negative regulation of BMP signaling pathway GO:0030514 IBA
    lipid droplet organization GO:0034389 IEA
    collateral sprouting in absence of injury GO:0048669 IEA
    negative regulation of collateral sprouting in absence of injury GO:0048698 IEA
    neuromuscular process GO:0050905 IEA
    cell division GO:0051301 IBA
    cell division GO:0051301 IMP
    regulation of mitochondrial membrane potential GO:0051881 IMP
    adipose tissue development GO:0060612 IEA
    lipophagy GO:0061724 IMP
    midbody abscission GO:0061952 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    mitochondrial outer membrane GO:0005741 IDA
    lipid droplet GO:0005811 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    midbody GO:0030496 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    synapse GO:0045202 IEA
 Experiment description of studies that identified SPG20 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SPG20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 REEP5 7905
Affinity Capture-MS Homo sapiens
2 CISD3 284106
Affinity Capture-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 DNAJC11 55735
Affinity Capture-MS Homo sapiens
5 ZFYVE9  
Two-hybrid Homo sapiens
6 RAD17  
Affinity Capture-MS Homo sapiens
7 UBAC1 10422
Two-hybrid Homo sapiens
8 AIFM1 9131
Affinity Capture-MS Homo sapiens
9 LAMTOR1 55004
Proximity Label-MS Homo sapiens
10 WWP1 11059
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
11 DESI2  
Two-hybrid Homo sapiens
12 LMNB1 4001
Affinity Capture-MS Homo sapiens
13 WWOX 51741
Affinity Capture-MS Homo sapiens
14 C9orf78 51759
Affinity Capture-MS Homo sapiens
15 VIM 7431
Affinity Capture-MS Homo sapiens
16 RABGEF1 27342
Two-hybrid Homo sapiens
17 USP5 8078
Two-hybrid Homo sapiens
18 KPNB1 3837
Affinity Capture-MS Homo sapiens
19 OTULIN 90268
Two-hybrid Homo sapiens
20 RAD23B 5887
Co-fractionation Homo sapiens
21 PRKAR1A 5573
Two-hybrid Homo sapiens
22 POLK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 HYPK 25764
Co-fractionation Homo sapiens
24 EPS15 2060
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
25 CUL3 8452
Affinity Capture-MS Homo sapiens
26 SMURF1 57154
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SQSTM1 8878
Proximity Label-MS Homo sapiens
28 UBC 7316
Affinity Capture-MS Homo sapiens
29 DNAJC25 548645
Affinity Capture-MS Homo sapiens
30 KIF5B 3799
Affinity Capture-MS Homo sapiens
31 MAP3K1 4214
Biochemical Activity Homo sapiens
32 CAPZB 832
Affinity Capture-MS Homo sapiens
33 CLTC 1213
Affinity Capture-MS Homo sapiens
34 COPA 1314
Affinity Capture-MS Homo sapiens
35 APP 351
Reconstituted Complex Homo sapiens
36 TUBA1A 7846
FRET Homo sapiens
37 TPT1 7178
Cross-Linking-MS (XL-MS) Homo sapiens
38 Ppp6r1  
Affinity Capture-MS Mus musculus
39 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
40 GPR35  
Two-hybrid Homo sapiens
41 HADHA 3030
Affinity Capture-MS Homo sapiens
42 WWP2 11060
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
43 HECW2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 MAPK6  
Affinity Capture-MS Homo sapiens
45 ACSL3 2181
Affinity Capture-MS Homo sapiens
46 NCL 4691
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
47 RPA3 6119
Proximity Label-MS Homo sapiens
48 SLX4  
Affinity Capture-MS Homo sapiens
49 COQ9  
Affinity Capture-MS Homo sapiens
50 SEC16A 9919
Affinity Capture-MS Homo sapiens
51 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
52 KLC2 64837
Affinity Capture-MS Homo sapiens
53 DTX2 113878
Proximity Label-MS Homo sapiens
54 PLIN3 10226
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
55 DPH5 51611
Co-fractionation Homo sapiens
56 IMPDH2 3615
Proximity Label-MS Homo sapiens
57 RPA4  
Proximity Label-MS Homo sapiens
58 C9orf72  
Affinity Capture-MS Homo sapiens
59 SERBP1 26135
Affinity Capture-MS Homo sapiens
60 SMURF2 64750
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
61 HSPD1 3329
Affinity Capture-MS Homo sapiens
62 HSPA9 3313
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
63 HSPA5 3309
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
64 ITCH 83737
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 OTUD7B 56957
Two-hybrid Homo sapiens
66 ACSL4 2182
Affinity Capture-MS Homo sapiens
67 AKAP1 8165
Proximity Label-MS Homo sapiens
68 EP300 2033
Affinity Capture-MS Homo sapiens
69 YOD1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SPG20 is involved
No pathways found





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