Gene description for DPH5
Gene name diphthamide biosynthesis 5
Gene symbol DPH5
Other names/aliases AD-018
CGI-30
HSPC143
NPD015
Species Homo sapiens
 Database cross references - DPH5
ExoCarta ExoCarta_51611
Vesiclepedia VP_51611
Entrez Gene 51611
HGNC 24270
MIM 611075
UniProt Q9H2P9  
 DPH5 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DPH5
Molecular Function
    diphthine methyl ester synthase activity GO:0141133 EXP
    diphthine methyl ester synthase activity GO:0141133 NAS
Biological Process
    protein histidyl modification to diphthamide GO:0017183 IEA
    protein histidyl modification to diphthamide GO:0017183 ISS
    protein histidyl modification to diphthamide GO:0017183 NAS
    protein histidyl modification to diphthamide GO:0017183 TAS
    methylation GO:0032259 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DPH5 in sEVs
1
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DPH5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SPG20 23111
Co-fractionation Homo sapiens
2 ZYX 7791
Co-fractionation Homo sapiens
3 NXF1 10482
Affinity Capture-RNA Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 ENO1 2023
Affinity Capture-RNA Homo sapiens
6 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
7 SFPQ 6421
Co-fractionation Homo sapiens
8 PARVA 55742
Co-fractionation Homo sapiens
9 CD2AP 23607
Co-fractionation Homo sapiens
10 UFD1L 7353
Co-fractionation Homo sapiens
11 FASN 2194
Positive Genetic Homo sapiens
12 GCLC 2729
Co-fractionation Homo sapiens
13 MOV10 4343
Affinity Capture-RNA Homo sapiens
14 HNRNPL 3191
Co-fractionation Homo sapiens
15 BICD2 23299
Proximity Label-MS Homo sapiens
16 NMD3 51068
Co-fractionation Homo sapiens
17 CCS 9973
Co-fractionation Homo sapiens
18 C12orf10 60314
Co-fractionation Homo sapiens
View the network image/svg+xml



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