Gene description for Cops5
Gene name COP9 (constitutive photomorphogenic) homolog, subunit 5 (Arabidopsis thaliana)
Gene symbol Cops5
Other names/aliases AI303502
CSN5
Jab1
Mov34
Sgn5
Species Mus musculus
 Database cross references - Cops5
ExoCarta ExoCarta_26754
Vesiclepedia VP_26754
Entrez Gene 26754
UniProt O35864  
 Cops5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cops5
Molecular Function
    transcription coactivator activity GO:0003713 TAS
    protein binding GO:0005515 IPI
    metallopeptidase activity GO:0008237 IBA
    metallopeptidase activity GO:0008237 ISO
    deNEDDylase activity GO:0019784 IBA
    enzyme binding GO:0019899 IPI
    macrophage migration inhibitory factor binding GO:0035718 IPI
    metal ion binding GO:0046872 IEA
    metal-dependent deubiquitinase activity GO:0140492 IEA
    metal-dependent deubiquitinase activity GO:0140492 ISO
Biological Process
    protein deneddylation GO:0000338 IEA
    protein deneddylation GO:0000338 ISO
    regulation of DNA-templated transcription GO:0006355 TAS
    proteolysis GO:0006508 IEA
    reproductive process GO:0022414 IBA
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    regulation of JNK cascade GO:0046328 IEA
    regulation of JNK cascade GO:0046328 ISO
    positive regulation of DNA-binding transcription factor activity GO:0051091 ISO
    positive regulation of DNA-binding transcription factor activity GO:0051091 ISS
    regulation of cell cycle GO:0051726 IBA
    regulation of cell cycle GO:0051726 IMP
    regulation of IRE1-mediated unfolded protein response GO:1903894 IEA
    regulation of IRE1-mediated unfolded protein response GO:1903894 ISO
    exosomal secretion GO:1990182 IEA
    exosomal secretion GO:1990182 ISO
Subcellular Localization
    chromatin GO:0000785 IEA
    chromatin GO:0000785 ISO
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    transcription regulator complex GO:0005667 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    synaptic vesicle GO:0008021 IEA
    synaptic vesicle GO:0008021 ISO
    COP9 signalosome GO:0008180 IBA
    COP9 signalosome GO:0008180 IDA
    COP9 signalosome GO:0008180 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
 Experiment description of studies that identified Cops5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cops5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rbx1  
Co-fractionation Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 CUL1 8454
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
4 GPS1 2873
Affinity Capture-Western Homo sapiens
5 Tulp3  
Co-fractionation Mus musculus
6 Id1  
Two-hybrid Mus musculus
7 Cdkn1b  
Two-hybrid Mus musculus
Co-localization Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
8 Ttc5  
Co-fractionation Mus musculus
9 Socs3  
Affinity Capture-Western Mus musculus
10 Cops7b  
Affinity Capture-Western Mus musculus
11 Vcp 269523
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
12 Chaf1a  
Affinity Capture-MS Mus musculus
13 Jun  
Two-hybrid Mus musculus
14 Uchl1 22223
Affinity Capture-Western Mus musculus
15 Id3  
Two-hybrid Mus musculus
Far Western Mus musculus
Reconstituted Complex Mus musculus
16 Xpo1 103573
Reconstituted Complex Mus musculus
17 Cops2 12848
Affinity Capture-Western Mus musculus
18 Cul4b  
Co-fractionation Mus musculus
19 Runx2  
Affinity Capture-MS Mus musculus
20 Ranbp9  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
21 Cops6 26893
Affinity Capture-Western Mus musculus
22 Tmod1  
Co-fractionation Mus musculus
23 Nudt16l1  
Co-fractionation Mus musculus
24 Zfr  
Co-fractionation Mus musculus
25 Cops8 108679
Affinity Capture-Western Mus musculus
26 MSRA 281312
Reconstituted Complex Bos taurus
27 Cops4 26891
Affinity Capture-Western Mus musculus
28 Gps1 209318
Affinity Capture-Western Mus musculus
29 HIF1A 3091
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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