Gene description for TMEM97
Gene name transmembrane protein 97
Gene symbol TMEM97
Other names/aliases MAC30
Species Homo sapiens
 Database cross references - TMEM97
ExoCarta ExoCarta_27346
Vesiclepedia VP_27346
Entrez Gene 27346
HGNC 28106
MIM 612912
UniProt Q5BJF2  
 TMEM97 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TMEM97
Molecular Function
    protein binding GO:0005515 IPI
    oxysterol binding GO:0008142 ISS
    cholesterol binding GO:0015485 ISS
Biological Process
    regulation of cell growth GO:0001558 NAS
    regulation of intracellular lipid transport GO:0032377 IMP
    regulation of intracellular cholesterol transport GO:0032383 IMP
    cholesterol homeostasis GO:0042632 IDA
    positive regulation of wound healing GO:0090303 IMP
    positive regulation of lipoprotein transport GO:0140077 IMP
Subcellular Localization
    lysosome GO:0005764 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 IDA
    rough endoplasmic reticulum GO:0005791 IDA
    plasma membrane GO:0005886 IDA
    rough endoplasmic reticulum membrane GO:0030867 IEA
    nuclear membrane GO:0031965 IDA
 Experiment description of studies that identified TMEM97 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TMEM97
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDUFAF1  
Two-hybrid Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 IFNGR2  
Two-hybrid Homo sapiens
4 REEP5 7905
Proximity Label-MS Homo sapiens
5 CLPP 8192
Proximity Label-MS Homo sapiens
6 C5AR2  
Affinity Capture-MS Homo sapiens
7 KIR2DL3  
Two-hybrid Homo sapiens
8 ASZ1  
Two-hybrid Homo sapiens
9 GPX8 493869
Two-hybrid Homo sapiens
10 DUSP1  
Affinity Capture-MS Homo sapiens
11 SGPL1 8879
Two-hybrid Homo sapiens
12 TMEM9 252839
Affinity Capture-MS Homo sapiens
13 FFAR2  
Two-hybrid Homo sapiens
14 PLP1 5354
Two-hybrid Homo sapiens
15 SSMEM1  
Two-hybrid Homo sapiens
16 PDZK1IP1 10158
Two-hybrid Homo sapiens
17 CLSTN3 9746
Two-hybrid Homo sapiens
18 GUK1 2987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 LAMP3  
Proximity Label-MS Homo sapiens
20 LRRC59 55379
Two-hybrid Homo sapiens
21 PANX1 24145
Proximity Label-MS Homo sapiens
22 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 NUP155 9631
Proximity Label-MS Homo sapiens
24 FRMD3  
Two-hybrid Homo sapiens
25 MTIF3  
Two-hybrid Homo sapiens
26 CD53 963
Two-hybrid Homo sapiens
27 SLC14A2  
Two-hybrid Homo sapiens
28 TMEM106C 79022
Two-hybrid Homo sapiens
29 SPINT1 6692
Two-hybrid Homo sapiens
30 ERGIC3 51614
Two-hybrid Homo sapiens
31 TMEM14B  
Two-hybrid Homo sapiens
32 KLRK1  
Affinity Capture-MS Homo sapiens
33 LAMP2 3920
Proximity Label-MS Homo sapiens
34 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
35 RNF170 81790
Two-hybrid Homo sapiens
36 EBP  
Two-hybrid Homo sapiens
37 NDUFAF2  
Two-hybrid Homo sapiens
38 MADCAM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 CLDN7 1366
Two-hybrid Homo sapiens
40 MRM1  
Two-hybrid Homo sapiens
41 CPLX4  
Two-hybrid Homo sapiens
42 APP 351
Reconstituted Complex Homo sapiens
43 DUSP6 1848
Affinity Capture-MS Homo sapiens
44 PDCD1LG2 80380
Two-hybrid Homo sapiens
45 LEUTX  
Two-hybrid Homo sapiens
46 GPR152  
Two-hybrid Homo sapiens
47 FAM209A 200232
Two-hybrid Homo sapiens
48 GJB5  
Two-hybrid Homo sapiens
49 HSD17B13  
Two-hybrid Homo sapiens
50 RPA3 6119
Proximity Label-MS Homo sapiens
51 PCDHGC4  
Affinity Capture-MS Homo sapiens
52 ATP2A1 487
Affinity Capture-MS Homo sapiens
53 EGFR 1956
Affinity Capture-MS Homo sapiens
54 GPR37L1  
Two-hybrid Homo sapiens
55 PSCA 8000
Two-hybrid Homo sapiens
56 RHCG 51458
Two-hybrid Homo sapiens
57 CIT 11113
Affinity Capture-MS Homo sapiens
58 LIME1 54923
Two-hybrid Homo sapiens
59 TM2D2 83877
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 TMEM45B 120224
Two-hybrid Homo sapiens
61 DUSP9  
Affinity Capture-MS Homo sapiens
62 C10orf54 64115
Two-hybrid Homo sapiens
63 MUC1 4582
Two-hybrid Homo sapiens
64 RPN1 6184
Proximity Label-MS Homo sapiens
65 VIPR1  
Affinity Capture-MS Homo sapiens
66 TMEM52B 120939
Two-hybrid Homo sapiens
67 ARL13B 200894
Two-hybrid Homo sapiens
68 GJB3  
Two-hybrid Homo sapiens
69 AQP12B  
Two-hybrid Homo sapiens
70 ATP6AP2 10159
Two-hybrid Homo sapiens
71 LHFPL5  
Two-hybrid Homo sapiens
72 MFF 56947
Two-hybrid Homo sapiens
73 TMEM194A  
Two-hybrid Homo sapiens
74 AQP2 359
Two-hybrid Homo sapiens
75 TMEM79  
Two-hybrid Homo sapiens
76 NAP1L5  
Affinity Capture-MS Homo sapiens
77 TMX2 51075
Two-hybrid Homo sapiens
78 SLC14A1  
Two-hybrid Homo sapiens
79 STXBP3 6814
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TMEM97 is involved
No pathways found





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