ExoCarta: Gene summary

Credits | Contact

Gene description for MRPL15
Gene name	mitochondrial ribosomal protein L15
Gene symbol	MRPL15
Other names/aliases	HSPC145 L15mt MRP-L15 MRP-L7 RPML7
Species	Homo sapiens
Database cross references - MRPL15
ExoCarta	ExoCarta_29088
Vesiclepedia	VP_29088
Entrez Gene	29088
HGNC	14054
MIM	611828
UniProt	Q9P015
MRPL15 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942

Gene ontology annotations for MRPL15

Molecular Function
	RNA binding	GO:0003723	HDA
	structural constituent of ribosome	GO:0003735	IBA
	protein binding	GO:0005515	IPI
Biological Process
	mitochondrial translation	GO:0032543	NAS
	cellular response to leukemia inhibitory factor	GO:1990830	IEA
Subcellular Localization
	mitochondrion	GO:0005739	HTP
	mitochondrion	GO:0005739	IDA
	mitochondrial inner membrane	GO:0005743	NAS
	mitochondrial inner membrane	GO:0005743	TAS
	mitochondrial large ribosomal subunit	GO:0005762	IBA
	mitochondrial large ribosomal subunit	GO:0005762	IDA
	mitochondrial large ribosomal subunit	GO:0005762	NAS

Experiment description of studies that identified MRPL15 in sEVs

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for MRPL15

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

UBE2H

7328

Affinity Capture-MS

Homo sapiens

NUPL2

11097

Affinity Capture-MS

Homo sapiens

RIT1

6016

Negative Genetic

Homo sapiens

H1FNT

Affinity Capture-MS

Homo sapiens

ZNF346

Affinity Capture-MS

Homo sapiens

RBMS3

Affinity Capture-MS

Homo sapiens

EIF3C

8663

Co-fractionation

Homo sapiens

MRPL30

Affinity Capture-MS

Homo sapiens

MRPL17

Affinity Capture-MS

Homo sapiens

NOP9

161424

Affinity Capture-MS

Homo sapiens

RBMS2

5939

Affinity Capture-MS

Homo sapiens

RPS14

6208

Co-fractionation

Homo sapiens

RPL13A

23521

Affinity Capture-MS

Homo sapiens

TRUB2

Proximity Label-MS

Homo sapiens

MTG2

Proximity Label-MS

Homo sapiens

NGRN

Proximity Label-MS

Homo sapiens

RPL10

6134

Affinity Capture-MS

Homo sapiens

CHCHD1

Proximity Label-MS

Homo sapiens

MRPL37

51253

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL27

51264

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL50

54534

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

CAND1

55832

Affinity Capture-MS

Homo sapiens

MRPL52

Affinity Capture-MS

Homo sapiens

RPUSD4

84881

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL49

740

Affinity Capture-MS

Homo sapiens

MARC1

Co-fractionation

Homo sapiens

MTRF1

Proximity Label-MS

Homo sapiens

GFM2

84340

Proximity Label-MS

Homo sapiens

HNRNPUL2

221092

Affinity Capture-MS

Homo sapiens

LRPPRC

10128

Proximity Label-MS

Homo sapiens

IGF2BP3

10643

Affinity Capture-MS

Homo sapiens

SNRNP70

6625

Affinity Capture-MS

Homo sapiens

MRPL20

55052

Co-fractionation

Homo sapiens

OGDH

4967

Co-fractionation

Homo sapiens

BTRC

8945

Affinity Capture-MS

Homo sapiens

MRPL40

64976

Co-fractionation

Homo sapiens

PMPCA

23203

Proximity Label-MS

Homo sapiens

UBE2O

63893

Affinity Capture-MS

Homo sapiens

ZNF485

Affinity Capture-MS

Homo sapiens

MRPL23

6150

Affinity Capture-MS

Homo sapiens

COPS5

10987

Affinity Capture-MS

Homo sapiens

HINT2

84681

Proximity Label-MS

Homo sapiens

DUSP11

Affinity Capture-MS

Homo sapiens

MRPL12

6182

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

KLF15

Affinity Capture-MS

Homo sapiens

MRPL57

Co-fractionation

Homo sapiens

MRPS26

64949

Proximity Label-MS

Homo sapiens

MALSU1

Affinity Capture-MS

Homo sapiens

TRA2A

29896

Affinity Capture-MS

Homo sapiens

EXD2

Proximity Label-MS

Homo sapiens

TMEM192

201931

Affinity Capture-MS

Homo sapiens

MRPL39

54148

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

KLF4

Affinity Capture-MS

Homo sapiens

CCNDBP1

23582

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

PMPCB

9512

Proximity Label-MS

Homo sapiens

MRPL4

51073

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FASTKD2

Proximity Label-MS

Homo sapiens

VWA8

23078

Proximity Label-MS

Homo sapiens

MRPL47

57129

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FAM120A

23196

Affinity Capture-MS

Homo sapiens

MTERF3

Proximity Label-MS

Homo sapiens

LIN28A

Affinity Capture-MS

Homo sapiens

MDH2

4191

Proximity Label-MS

Homo sapiens

MRPS30

10884

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL13

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL32

64983

Co-fractionation

Homo sapiens

SCFD1

23256

Co-fractionation

Homo sapiens

C6orf203

Proximity Label-MS

Homo sapiens

NDUFS4

4724

Co-fractionation

Homo sapiens

COX15

1355

Co-fractionation

Homo sapiens

FASTKD5

Proximity Label-MS

Homo sapiens

MRPL45

84311

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPL26

6154

Affinity Capture-MS

Homo sapiens

TMEM177

Co-fractionation

Homo sapiens

SRSF3

6428

Affinity Capture-MS

Homo sapiens

YBX2

51087

Affinity Capture-MS

Homo sapiens

TUFM

7284

Proximity Label-MS

Homo sapiens

MTIF3

Proximity Label-MS

Homo sapiens

HSCB

150274

Proximity Label-MS

Homo sapiens

HIST1H2AM

8336

Affinity Capture-MS

Homo sapiens

RPL35

11224

Affinity Capture-MS

Homo sapiens

NTRK1

4914

Affinity Capture-MS

Homo sapiens

CCDC109B

55013

Proximity Label-MS

Homo sapiens

MOV10

4343

Affinity Capture-RNA

Homo sapiens

CBX6

Affinity Capture-MS

Homo sapiens

CYC1

1537

Co-fractionation

Homo sapiens

MRPL3

Co-fractionation

Homo sapiens

RUNDC3A

Two-hybrid

Homo sapiens

MRPL11

65003

Co-fractionation		Homo sapiens
Proximity Label-MS		Homo sapiens

MTRF1L

Proximity Label-MS

Homo sapiens

TMEM70

Proximity Label-MS

Homo sapiens

CYLD

Affinity Capture-MS

Homo sapiens

MRPL2

51069

Affinity Capture-MS

Homo sapiens

MRPL41

64975

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL14

64928

Co-fractionation

Homo sapiens

MRPL44

Affinity Capture-MS

Homo sapiens

MRPL51

51258

Affinity Capture-MS

Homo sapiens

RHOU

58480

Reconstituted Complex

Homo sapiens

MRRF

Proximity Label-MS

Homo sapiens

100

ABT1

29777

Affinity Capture-MS

Homo sapiens

101

MRPS12

Proximity Label-MS

Homo sapiens

102

MRPL46

Affinity Capture-MS

Homo sapiens

103

MTIF2

4528

Proximity Label-MS

Homo sapiens

104

RPS2

6187

Co-fractionation

Homo sapiens

105

MRPL35

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

106

RBM47

54502

Affinity Capture-MS

Homo sapiens

107

MRPL10

124995

Co-fractionation		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

108

SRSF4

6429

Affinity Capture-MS

Homo sapiens

109

MRPL9

65005

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

110

MRPL16

Co-fractionation

Homo sapiens

111

RPL37

6167

Affinity Capture-MS

Homo sapiens

112

SRSF5

6430

Affinity Capture-MS

Homo sapiens

113

FKBP8

23770

Co-fractionation

Homo sapiens

114

GADD45GIP1

Affinity Capture-MS

Homo sapiens

115

HNRNPA1

3178

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

116

FAHD1

81889

Affinity Capture-MS

Homo sapiens

117

NXF1

10482

Affinity Capture-RNA

Homo sapiens

118

MRPL1

Affinity Capture-MS

Homo sapiens

119

MYCN

Affinity Capture-MS

Homo sapiens

120

PRR3

Affinity Capture-MS

Homo sapiens

121

TACO1

Proximity Label-MS

Homo sapiens

122

MTG1

Proximity Label-MS

Homo sapiens

123

MYC

Affinity Capture-MS

Homo sapiens

124

DLST

1743

Co-fractionation

Homo sapiens

125

TSFM

10102

Proximity Label-MS

Homo sapiens

126

ACAD9

28976

Proximity Label-MS

Homo sapiens

127

SRSF10

10772

Affinity Capture-MS

Homo sapiens

128

MRPS17

51373

Affinity Capture-MS

Homo sapiens

129

MRPL38

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Co-fractionation		Homo sapiens

130

METTL15

Proximity Label-MS

Homo sapiens

131

ZC3H3

Affinity Capture-MS

Homo sapiens

132

DNAJC15

Proximity Label-MS

Homo sapiens

133

GNL3

26354

Affinity Capture-MS

Homo sapiens

134

SOX2

Affinity Capture-MS

Homo sapiens

135

RPL19

6143

Affinity Capture-MS

Homo sapiens

136

RPL13

6137

Affinity Capture-MS

Homo sapiens

137

YBX1

4904

Affinity Capture-MS

Homo sapiens

138

1431

Proximity Label-MS

Homo sapiens

139

MRPL42

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

140

PLEKHA4

57664

Affinity Capture-MS

Homo sapiens

141

RC3H1

149041

Affinity Capture-MS

Homo sapiens

142

AUH

549

Proximity Label-MS

Homo sapiens

143

ILF3

3609

Co-fractionation

Homo sapiens

144

RPL6

6128

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

145

ICT1

3396

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens
Cross-Linking-MS (XL-MS)		Homo sapiens

146

APOBEC3D

Affinity Capture-MS

Homo sapiens

147

MRPL53

Affinity Capture-MS

Homo sapiens

148

MRPL43

84545

Affinity Capture-MS

Homo sapiens

149

C12orf65

Proximity Label-MS

Homo sapiens

150

NDUFA12

55967

Co-fractionation

Homo sapiens

151

C21orf33

Proximity Label-MS

Homo sapiens

152

MRPL18

29074

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

153

SSBP1

6742

Proximity Label-MS

Homo sapiens

154

FASTKD3

Proximity Label-MS

Homo sapiens

155

NEIL1

Affinity Capture-MS

Homo sapiens

156

GFM1

85476

Proximity Label-MS

Homo sapiens

157

SRSF7

6432

Affinity Capture-MS

Homo sapiens

158

MRPL19

9801

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

159

RPS8

6202

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

160

TBRG4

9238

Proximity Label-MS

Homo sapiens

161

GRSF1

2926

Proximity Label-MS

Homo sapiens

162

C1QBP

708

Proximity Label-MS

Homo sapiens

163

RPL23A

6147

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

164

KRAS

3845

Negative Genetic

Homo sapiens

165

HNRNPU

3192

Affinity Capture-MS

Homo sapiens

166

MRPL55

Co-fractionation

Homo sapiens

167

C17orf80

55028

Proximity Label-MS

Homo sapiens

168

METTL17

Proximity Label-MS

Homo sapiens

169

MRPL28

10573

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

170

MRPS18A

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

171

DDX28

Proximity Label-MS

Homo sapiens

172

RPL17

6139

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which MRPL15 is involved

Pathway	Evidence	Source
Metabolism of proteins	TAS	Reactome
Metabolism of proteins	IEA	Reactome
Mitochondrial translation	TAS	Reactome
Mitochondrial translation	IEA	Reactome
Mitochondrial translation elongation	TAS	Reactome
Mitochondrial translation elongation	IEA	Reactome
Mitochondrial translation initiation	IEA	Reactome
Mitochondrial translation termination	TAS	Reactome
Translation	TAS	Reactome
Translation	IEA	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here