 Experiment description of studies that identified HIST1H2AD in sEVs |
| 1 |
| Experiment ID |
282 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Ultracentrifugation - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 2 |
| Experiment ID |
283 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 3 |
| Experiment ID |
285 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 4 |
| Experiment ID |
286 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 5 |
| Experiment ID |
15 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
17486113
|
| Organism |
Mus musculus
Homo sapiens |
| Experiment description |
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. |
| Authors |
"Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" |
| Journal name |
NCB
|
| Publication year |
2007 |
| Sample |
Mast cells |
| Sample name |
MC9
Bone marrow-derived mast cells
HMC-1 |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.11-1.21 g/mL
|
| Molecules identified in the study |
Protein
mRNA
miRNA |
| Methods used in the study |
Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array |
|
|
| 6 |
| Experiment ID |
224 |
| MISEV standards |
|
✔
EM|AFM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25944692
|
| Organism |
Homo sapiens |
| Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
| Authors |
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Neuroblastoma cells |
| Sample name |
SH-SY5Y |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
OptiPrep density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 7 |
| Experiment ID |
138 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 8 |
| Experiment ID |
140 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
VCaP - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 9 |
| Experiment ID |
141 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
VCaP - Rep 3 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 10 |
| Experiment ID |
142 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
LNCaP - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 11 |
| Experiment ID |
144 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
C4-2 - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 12 |
| Experiment ID |
145 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|CD10
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
C4-2 - Rep 3 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 13 |
| Experiment ID |
146 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
RWPE - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 14 |
| Experiment ID |
274 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
|
Enriched markers |
|
✔
AIF
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25844599
|
| Organism |
Homo sapiens |
| Experiment description |
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. |
| Authors |
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T." |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Docetaxel resistant |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.18 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Flow cytometry
Western blotting |
|
|
| 15 |
| Experiment ID |
834 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 16 |
| Experiment ID |
835 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Retinal pigment epithelial cells |
| Sample name |
ARPE-19 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 17 |
| Experiment ID |
191 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
Alix|CD81|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20124223
|
| Organism |
Homo sapiens |
| Experiment description |
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. |
| Authors |
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2010 |
| Sample |
Squamous carcinoma cells |
| Sample name |
Squamous carcinoma cell (A431) |
| Isolation/purification methods |
Differential centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
