Experiment description of studies that identified DNAJB3 in sEVs

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

Protein-protein interactions for DNAJB3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 DNAJC7 7266 Affinity Capture-MS Homo sapiens 2 BAG3 9531 Affinity Capture-Luminescence Homo sapiens 3 ATP5H 10476 Cross-Linking-MS (XL-MS) Homo sapiens 4 HIST1H2AD 3013 Cross-Linking-MS (XL-MS) Homo sapiens 5 GINS2   Affinity Capture-MS Homo sapiens 6 TRAF3IP2   Affinity Capture-MS Homo sapiens 7 FAM175B 23172 Proximity Label-MS Homo sapiens 8 HSPA6 3310 Affinity Capture-MS Homo sapiens 9 KIF14 9928 Affinity Capture-MS Homo sapiens 10 HSPA8 3312 Affinity Capture-MS Homo sapiens 11 COPS4 51138 Proximity Label-MS Homo sapiens 12 PGRMC1 10857 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 13 TBCE 6905 Affinity Capture-MS Homo sapiens 14 GPX1 2876 Affinity Capture-MS Homo sapiens 15 BAG5 9529 Affinity Capture-MS Homo sapiens 16 HSP90AB4P 664618 Affinity Capture-MS Homo sapiens 17 HIST1H2BJ 8970 Cross-Linking-MS (XL-MS) Homo sapiens 18 HYPK 25764 Affinity Capture-MS Homo sapiens 19 TRIM65 201292 Affinity Capture-MS Homo sapiens 20 FBXO10   Affinity Capture-MS Homo sapiens 21 GRPEL1 80273 Affinity Capture-MS Homo sapiens 22 DNPEP 23549 Affinity Capture-MS Homo sapiens 23 CAPRIN1 4076 Affinity Capture-MS Homo sapiens 24 PCBD1 5092 Affinity Capture-MS Homo sapiens 25 HNRNPA1 3178 Affinity Capture-MS Homo sapiens 26 C19orf25   Affinity Capture-MS Homo sapiens 27 HIST1H2AG 8969 Cross-Linking-MS (XL-MS) Homo sapiens 28 SIRT1   Proximity Label-MS Homo sapiens 29 SOX2   Affinity Capture-MS Homo sapiens 30 DYNC1I2 1781 Affinity Capture-MS Homo sapiens 31 GINS3   Affinity Capture-MS Homo sapiens 32 UFL1 23376 Affinity Capture-MS Homo sapiens 33 TMEM231 79583 Affinity Capture-MS Homo sapiens 34 SCYL1 57410 Affinity Capture-MS Homo sapiens 35 BBS7 55212 Proximity Label-MS Homo sapiens 36 VPS26B 112936 Affinity Capture-MS Homo sapiens 37 UTP6   Affinity Capture-MS Homo sapiens 38 TCAF1 9747 Affinity Capture-MS Homo sapiens 39 CHEK1   Affinity Capture-MS Homo sapiens 40 DNAJA1 3301 Proximity Label-MS Homo sapiens 41 HSPA4 3308 Affinity Capture-MS Homo sapiens 42 C1D   Affinity Capture-MS Homo sapiens 43 GINS1   Affinity Capture-MS Homo sapiens 44 AIP 9049 Affinity Capture-MS Homo sapiens 45 EDRF1   Affinity Capture-MS Homo sapiens 46 SEH1L 81929 Proximity Label-MS Homo sapiens 47 MEIS1   Affinity Capture-MS Homo sapiens 48 ARGLU1   Affinity Capture-MS Homo sapiens 49 PTER 9317 Affinity Capture-MS Homo sapiens 50 HIST1H2BK 85236 Cross-Linking-MS (XL-MS) Homo sapiens 51 EWSR1 2130 Two-hybrid Homo sapiens 52 HSPA9 3313 Affinity Capture-MS Homo sapiens 53 HIST1H2BC 8347 Cross-Linking-MS (XL-MS) Homo sapiens 54 ANKRD13A 88455 Affinity Capture-MS Homo sapiens 55 NXT1   Affinity Capture-MS Homo sapiens View the network

Pathways in which DNAJB3 is involved

No pathways found