Gene description for TBCE
Gene name tubulin folding cofactor E
Gene symbol TBCE
Other names/aliases HRD
KCS
KCS1
pac2
Species Homo sapiens
 Database cross references - TBCE
ExoCarta ExoCarta_6905
Vesiclepedia VP_6905
Entrez Gene 6905
HGNC 11582
MIM 604934
UniProt Q15813  
 TBCE identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for TBCE
Molecular Function
    protein binding GO:0005515 IPI
    alpha-tubulin binding GO:0043014 IBA
    protein-folding chaperone binding GO:0051087 TAS
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    microtubule cytoskeleton organization GO:0000226 IMP
    protein folding GO:0006457 TAS
    tubulin complex assembly GO:0007021 IBA
    post-chaperonin tubulin folding pathway GO:0007023 IBA
    post-chaperonin tubulin folding pathway GO:0007023 IDA
    mitotic spindle organization GO:0007052 IMP
    adult locomotory behavior GO:0008344 IEA
    post-embryonic development GO:0009791 IEA
    muscle atrophy GO:0014889 IEA
    developmental growth GO:0048589 IEA
    peripheral nervous system neuron axonogenesis GO:0048936 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    microtubule GO:0005874 TAS
 Experiment description of studies that identified TBCE in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TBCE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 ARL2 402
Co-fractionation Homo sapiens
3 NTRK1 4914
Affinity Capture-MS Homo sapiens
4 RNF7  
Affinity Capture-MS Homo sapiens
5 TUBB 203068
Co-fractionation Homo sapiens
6 ASF1A 25842
Proximity Label-MS Homo sapiens
7 PRKY  
Affinity Capture-MS Homo sapiens
8 BKRF1  
Affinity Capture-MS
9 AHCY 191
Co-fractionation Homo sapiens
10 RAD23A 5886
Co-fractionation Homo sapiens
11 OGFOD1  
Co-fractionation Homo sapiens
12 IFT20 90410
Affinity Capture-MS Homo sapiens
13 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 AKT1 207
Biochemical Activity Homo sapiens
15 PYGL 5836
Co-fractionation Homo sapiens
16 BAG2 9532
Affinity Capture-MS Homo sapiens
17 SFTA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 C1orf168  
Affinity Capture-MS Homo sapiens
19 NFKBIA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 FGB 2244
Affinity Capture-MS Homo sapiens
21 TUBB4B 10383
Affinity Capture-MS Homo sapiens
22 CYP1A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TMEM263 90488
Affinity Capture-MS Homo sapiens
24 ARF6 382
Co-fractionation Homo sapiens
25 ANKRD39  
Affinity Capture-MS Homo sapiens
26 DNAJB3 414061
Affinity Capture-MS Homo sapiens
27 BHMT2 23743
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 APEX1 328
Affinity Capture-RNA Homo sapiens
29 MLF1  
Affinity Capture-MS Homo sapiens
30 ACACA 31
Positive Genetic Homo sapiens
31 PARK2  
Affinity Capture-MS Homo sapiens
32 IRF2BP1 26145
Affinity Capture-MS Homo sapiens
33 NMT1 4836
Affinity Capture-MS Homo sapiens
34 CLCC1 23155
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 BICD2 23299
Proximity Label-MS Homo sapiens
36 OGT 8473
Reconstituted Complex Homo sapiens
37 EGFR 1956
Negative Genetic Homo sapiens
38 CENPM  
Affinity Capture-MS Homo sapiens
39 RPL35A 6165
Affinity Capture-MS Homo sapiens
40 HSPA1A 3303
Affinity Capture-MS Homo sapiens
41 SERBP1 26135
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 TRIM11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ACTB 60
Proximity Label-MS Homo sapiens
44 KRAS 3845
Negative Genetic Homo sapiens
45 DIRAS1 148252
Affinity Capture-MS Homo sapiens
46 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 TRNT1 51095
Co-fractionation Homo sapiens
48 RPA3 6119
Proximity Label-MS Homo sapiens
49 GNPDA1 10007
Co-fractionation Homo sapiens
50 EDRF1  
Affinity Capture-MS Homo sapiens
51 DNAJC13 23317
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TBCE is involved
PathwayEvidenceSource
Metabolism of proteins IEA Reactome
Post-chaperonin tubulin folding pathway IEA Reactome
Protein folding IEA Reactome





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