Gene description for IL18
Gene name interleukin 18
Gene symbol IL18
Other names/aliases IGIF
IL-18
IL-1g
IL1F4
Species Homo sapiens
 Database cross references - IL18
ExoCarta ExoCarta_3606
Vesiclepedia VP_3606
Entrez Gene 3606
HGNC 5986
MIM 600953
UniProt Q14116  
 IL18 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IL18
Molecular Function
    cytokine activity GO:0005125 IBA
    cytokine activity GO:0005125 IDA
    cytokine activity GO:0005125 ISS
    cytokine activity GO:0005125 TAS
    protein binding GO:0005515 IPI
    interleukin-18 receptor binding GO:0045515 IDA
Biological Process
    angiogenesis GO:0001525 IDA
    cytokine production GO:0001816 IBA
    inflammatory response GO:0006954 IBA
    inflammatory response GO:0006954 IEA
    immune response GO:0006955 IBA
    cell-cell signaling GO:0007267 TAS
    cell population proliferation GO:0008283 IEA
    positive regulation of macrophage derived foam cell differentiation GO:0010744 ISS
    cytokine-mediated signaling pathway GO:0019221 IBA
    natural killer cell activation GO:0030101 IEA
    regulation of cell adhesion GO:0030155 IDA
    sleep GO:0030431 ISS
    positive regulation of chemokine production GO:0032722 TAS
    positive regulation of granulocyte macrophage colony-stimulating factor production GO:0032725 IDA
    positive regulation of type II interferon production GO:0032729 IDA
    positive regulation of type II interferon production GO:0032729 TAS
    positive regulation of interleukin-13 production GO:0032736 TAS
    positive regulation of interleukin-17 production GO:0032740 IDA
    positive regulation of natural killer cell proliferation GO:0032819 IDA
    positive regulation of tissue remodeling GO:0034105 IC
    interleukin-18-mediated signaling pathway GO:0035655 IDA
    T-helper 1 type immune response GO:0042088 IDA
    type 2 immune response GO:0042092 TAS
    positive regulation of activated T cell proliferation GO:0042104 IDA
    neutrophil activation GO:0042119 IEA
    natural killer cell mediated cytotoxicity GO:0042267 IEA
    positive regulation of tyrosine phosphorylation of STAT protein GO:0042531 IDA
    cholesterol homeostasis GO:0042632 ISS
    positive regulation of T-helper 2 cell differentiation GO:0045630 ISS
    negative regulation of myoblast differentiation GO:0045662 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of smooth muscle cell proliferation GO:0048661 IDA
    positive regulation of inflammatory response GO:0050729 IC
    positive regulation of inflammatory response GO:0050729 ISS
    defense response to Gram-positive bacterium GO:0050830 ISS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    positive regulation of NK T cell proliferation GO:0051142 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    establishment of skin barrier GO:0061436 ISS
    triglyceride homeostasis GO:0070328 ISS
    cellular response to lipopolysaccharide GO:0071222 IBA
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
    positive regulation of neuroinflammatory response GO:0150078 TAS
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 IEA
    positive regulation of T-helper 1 cell cytokine production GO:2000556 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified IL18 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IL18
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CASP4  
Biochemical Activity Homo sapiens
2 ZNF747  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 Cul1 26965
Affinity Capture-MS Mus musculus
4 SERPINB4 6318
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 IL18BP  
Reconstituted Complex Homo sapiens
6 TPT1 7178
Co-fractionation Homo sapiens
7 CASK 8573
Two-hybrid Homo sapiens
8 DFFA 1676
Co-fractionation Homo sapiens
9 IL1B  
Affinity Capture-MS Homo sapiens
10 GPX4 2879
Affinity Capture-MS Homo sapiens
11 CDK15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 EIF4A3 9775
Co-fractionation Homo sapiens
13 ADIRF 10974
Two-hybrid Homo sapiens
14 CASP1  
Biochemical Activity Homo sapiens
15 CDH1 999
Co-fractionation Homo sapiens
16 DIAPH1 1729
Co-fractionation Homo sapiens
17 FAM24B  
Affinity Capture-MS Homo sapiens
18 SHMT2 6472
Affinity Capture-RNA Homo sapiens
19 TDGF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 CCDC53 51019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RAC1 5879
Affinity Capture-MS Homo sapiens
22 STX17 55014
Affinity Capture-MS Homo sapiens
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