Gene description for CCDC88C
Gene name coiled-coil domain containing 88C
Gene symbol CCDC88C
Other names/aliases DAPLE
HKRP2
KIAA1509
SCA40
Species Homo sapiens
 Database cross references - CCDC88C
ExoCarta ExoCarta_440193
Vesiclepedia VP_440193
Entrez Gene 440193
HGNC 19967
MIM 611204
UniProt Q9P219  
 CCDC88C identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocytes 26054723    
 Gene ontology annotations for CCDC88C
Molecular Function
    G-protein alpha-subunit binding GO:0001965 IPI
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    frizzled binding GO:0005109 IPI
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IBA
    PDZ domain binding GO:0030165 ISS
    identical protein binding GO:0042802 ISS
    protein dimerization activity GO:0046983 IEA
    dynein light intermediate chain binding GO:0051959 IBA
Biological Process
    microtubule bundle formation GO:0001578 IEA
    apical constriction GO:0003383 IDA
    negative regulation of microtubule depolymerization GO:0007026 IEA
    small GTPase-mediated signal transduction GO:0007264 IDA
    cytoskeleton-dependent intracellular transport GO:0030705 IBA
    cytoplasmic microtubule organization GO:0031122 IBA
    protein destabilization GO:0031648 ISS
    non-canonical Wnt signaling pathway GO:0035567 IMP
    cilium organization GO:0044782 IEA
    positive regulation of JNK cascade GO:0046330 IMP
    Wnt signaling pathway, planar cell polarity pathway GO:0060071 IEA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IMP
    mucociliary clearance GO:0120197 IEA
    respiratory basal cell differentiation GO:1902691 IEA
Subcellular Localization
    extracellular region GO:0005576 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IBA
    cell junction GO:0030054 IDA
    cell junction GO:0030054 IMP
    apical junction complex GO:0043296 IEA
 Experiment description of studies that identified CCDC88C in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for CCDC88C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
3 MCM7 4176
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LYN 4067
Proximity Label-MS Homo sapiens
5 FZD6 8323
Reconstituted Complex Homo sapiens
6 DVL1 1855
Reconstituted Complex Homo sapiens
7 TCP10  
Affinity Capture-MS Homo sapiens
8 FZD7 8324
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 RNASE7 84659
Affinity Capture-MS Homo sapiens
10 ALDH1L1 10840
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ALOXE3  
Affinity Capture-MS Homo sapiens
12 SIKE1  
Affinity Capture-MS Homo sapiens
13 SYCE1  
Affinity Capture-MS Homo sapiens
14 NINL  
Proximity Label-MS Homo sapiens
15 PARD3 56288
Proximity Label-MS Homo sapiens
16 CTNNB1 1499
Proximity Label-MS Homo sapiens
17 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
18 SMURF1 57154
Affinity Capture-MS Homo sapiens
19 SQSTM1 8878
Affinity Capture-Western Homo sapiens
20 PRMT1 3276
Affinity Capture-MS Homo sapiens
21 TRIM11  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 KATNA1 11104
Affinity Capture-MS Homo sapiens
23 HIST1H2AG 8969
Cross-Linking-MS (XL-MS) Homo sapiens
24 DDX58 23586
Affinity Capture-RNA Homo sapiens
25 APP 351
Reconstituted Complex Homo sapiens
26 LATS1  
Proximity Label-MS Homo sapiens
27 CCT7 10574
Cross-Linking-MS (XL-MS) Homo sapiens
28 DYNC1H1 1778
Affinity Capture-Western Homo sapiens
29 HIST2H2AA3 8337
Cross-Linking-MS (XL-MS) Homo sapiens
30 ITSN2 50618
Two-hybrid Homo sapiens
31 WHSC1 7468
Affinity Capture-MS Homo sapiens
32 MLLT4 4301
Proximity Label-MS Homo sapiens
33 CEP135  
Proximity Label-MS Homo sapiens
34 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
35 DCTN1 1639
Affinity Capture-Western Homo sapiens
36 BICD1 636
Proximity Label-MS Homo sapiens
37 RHOB 388
Proximity Label-MS Homo sapiens
38 CTNNA1 1495
Proximity Label-MS Homo sapiens
39 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
40 MAPK8 5599
Two-hybrid Homo sapiens
41 LATS2 26524
Proximity Label-MS Homo sapiens
42 MPDZ  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
43 WDFY1 57590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GNAI3 2773
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
45 HNRNPA2B1 3181
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here