Gene description for FZD6
Gene name frizzled class receptor 6
Gene symbol FZD6
Other names/aliases FZ-6
FZ6
HFZ6
NDNC10
Species Homo sapiens
 Database cross references - FZD6
ExoCarta ExoCarta_8323
Vesiclepedia VP_8323
Entrez Gene 8323
HGNC 4044
MIM 603409
UniProt O60353  
 FZD6 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for FZD6
Molecular Function
    amyloid-beta binding GO:0001540 NAS
    G protein-coupled receptor activity GO:0004930 IEA
    protein binding GO:0005515 IPI
    Wnt-protein binding GO:0017147 IBA
    Wnt-protein binding GO:0017147 ISS
    Wnt-protein binding GO:0017147 NAS
    ubiquitin protein ligase binding GO:0031625 IPI
    Wnt receptor activity GO:0042813 IBA
    Wnt receptor activity GO:0042813 IDA
    Wnt receptor activity GO:0042813 NAS
Biological Process
    neural tube closure GO:0001843 IEA
    hair follicle development GO:0001942 IEA
    G protein-coupled receptor signaling pathway GO:0007186 IEA
    platelet activation GO:0030168 IEA
    cell proliferation in midbrain GO:0033278 IEA
    non-canonical Wnt signaling pathway GO:0035567 IBA
    non-canonical Wnt signaling pathway GO:0035567 IDA
    embryonic nail plate morphogenesis GO:0035880 IEA
    inner ear morphogenesis GO:0042472 IEA
    negative regulation of DNA-binding transcription factor activity GO:0043433 IDA
    establishment of body hair planar orientation GO:0048105 IEA
    canonical Wnt signaling pathway GO:0060070 IBA
    Wnt signaling pathway, planar cell polarity pathway GO:0060071 NAS
    negative regulation of canonical Wnt signaling pathway GO:0090090 IMP
    midbrain morphogenesis GO:1904693 TAS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IEA
    apical plasma membrane GO:0016324 IEA
    apicolateral plasma membrane GO:0016327 IEA
    cytoplasmic vesicle membrane GO:0030659 IEA
 Experiment description of studies that identified FZD6 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for FZD6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NKAIN1  
Affinity Capture-MS Homo sapiens
2 CANX 821
Affinity Capture-MS Homo sapiens
3 TMEM95  
Affinity Capture-MS Homo sapiens
4 VNN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CHRNA4  
Affinity Capture-MS Homo sapiens
6 GABRE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 HLA-G 3135
Affinity Capture-MS Homo sapiens
8 GP9 2815
Affinity Capture-MS Homo sapiens
9 ZDHHC12  
Affinity Capture-MS Homo sapiens
10 LRRC55  
Affinity Capture-MS Homo sapiens
11 GGH 8836
Affinity Capture-MS Homo sapiens
12 Itgb1 16412
Affinity Capture-MS Mus musculus
13 CHRNA1  
Affinity Capture-MS Homo sapiens
14 TMPRSS12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CD1A 909
Affinity Capture-MS Homo sapiens
16 SYNE4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ECEL1  
Affinity Capture-MS Homo sapiens
18 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
19 GPR114  
Affinity Capture-MS Homo sapiens
20 CCDC88C 440193
Reconstituted Complex Homo sapiens
21 KLRAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SFTPC  
Affinity Capture-MS Homo sapiens
23 SCN4A  
Affinity Capture-MS Homo sapiens
24 B3GAT1  
Proximity Label-MS Homo sapiens
25 SIRPD  
Affinity Capture-MS Homo sapiens
26 CD79A  
Affinity Capture-MS Homo sapiens
27 TMEM132A 54972
Affinity Capture-MS Homo sapiens
28 ZNRF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 UGT1A7 54577
Affinity Capture-MS Homo sapiens
30 PSCA 8000
Affinity Capture-MS Homo sapiens
31 ITM2C 81618
Affinity Capture-MS Homo sapiens
32 PTPRN  
Affinity Capture-MS Homo sapiens
33 GXYLT1 283464
Affinity Capture-MS Homo sapiens
34 KLRC1  
Affinity Capture-MS Homo sapiens
35 CD3E 916
Affinity Capture-MS Homo sapiens
36 SFRP1 6422
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 OR1D4  
Affinity Capture-MS Homo sapiens
38 CLEC4E  
Affinity Capture-MS Homo sapiens
39 C3orf52  
Affinity Capture-MS Homo sapiens
40 EP300 2033
Affinity Capture-MS Homo sapiens
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