Gene description for NDUFB6
Gene name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa
Gene symbol NDUFB6
Other names/aliases B17
CI
Species Homo sapiens
 Database cross references - NDUFB6
ExoCarta ExoCarta_4712
Vesiclepedia VP_4712
Entrez Gene 4712
HGNC 7701
MIM 603322
UniProt O95139  
 NDUFB6 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NDUFB6
Molecular Function
    protein binding GO:0005515 IPI
    NADH dehydrogenase (ubiquinone) activity GO:0008137 NAS
Biological Process
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 NAS
    aerobic respiration GO:0009060 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 IMP
    mitochondrial ATP synthesis coupled electron transport GO:0042775 IBA
    mitochondrial ATP synthesis coupled electron transport GO:0042775 IMP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial membrane GO:0031966 IDA
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 IMP
    respiratory chain complex I GO:0045271 NAS
 Experiment description of studies that identified NDUFB6 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NDUFB6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRELD1 78987
Affinity Capture-MS Homo sapiens
2 Kif19a  
Affinity Capture-MS Mus musculus
3 ERGIC3 51614
Two-hybrid Homo sapiens
4 SNCA 6622
Affinity Capture-Western Homo sapiens
5 GJA8  
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 NDUFA11 126328
Affinity Capture-MS Homo sapiens
8 NDUFV3 4731
Affinity Capture-MS Homo sapiens
9 NDUFS5 4725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 NDUFA1 4694
Affinity Capture-MS Homo sapiens
11 NDUFA13 51079
Co-fractionation Homo sapiens
12 NDUFA9 4704
Affinity Capture-MS Homo sapiens
13 NDUFV1 4723
Co-fractionation Homo sapiens
14 GALNT6 11226
Affinity Capture-MS Homo sapiens
15 NDUFB7 4713
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
16 MPV17 4358
Co-fractionation Homo sapiens
17 NDUFB5 4711
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 IKBIP 121457
Co-fractionation Homo sapiens
19 NDUFS7 374291
Affinity Capture-MS Homo sapiens
20 NDUFA8 4702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 APP 351
Reconstituted Complex Homo sapiens
22 MRPS28  
Co-fractionation Homo sapiens
23 NF1 4763
Negative Genetic Homo sapiens
24 PON1 5444
Affinity Capture-MS Homo sapiens
25 NDUFS3 4722
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 NDUFB3 4709
Affinity Capture-MS Homo sapiens
27 RPA3 6119
Proximity Label-MS Homo sapiens
28 SNX17 9784
Affinity Capture-MS Homo sapiens
29 SDHB 6390
Co-fractionation Homo sapiens
30 NDUFA7 4701
Affinity Capture-MS Homo sapiens
31 TIMMDC1  
Affinity Capture-MS Homo sapiens
32 STK11 6794
Negative Genetic Homo sapiens
33 CREB3  
Two-hybrid Homo sapiens
34 NDUFB11 54539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 NDUFA12 55967
Affinity Capture-MS Homo sapiens
37 DNAJC30  
Affinity Capture-MS Homo sapiens
38 NDUFV2 4729
Affinity Capture-MS Homo sapiens
39 NDUFC2 4718
Affinity Capture-MS Homo sapiens
40 EMC3 55831
Affinity Capture-MS Homo sapiens
41 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 FUT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 TMEM192 201931
Affinity Capture-MS Homo sapiens
44 ABCB7 22
Co-fractionation Homo sapiens
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