Gene description for APH1A
Gene name APH1A gamma secretase subunit
Gene symbol APH1A
Other names/aliases 6530402N02Rik
APH-1
APH-1A
CGI-78
Species Homo sapiens
 Database cross references - APH1A
ExoCarta ExoCarta_51107
Vesiclepedia VP_51107
Entrez Gene 51107
HGNC 29509
MIM 607629
UniProt Q96BI3  
 APH1A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for APH1A
Molecular Function
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IEA
    protein-macromolecule adaptor activity GO:0030674 IBA
    protein-macromolecule adaptor activity GO:0030674 IMP
    endopeptidase activator activity GO:0061133 IGI
    endopeptidase activator activity GO:0061133 IMP
Biological Process
    metanephros development GO:0001656 IEA
    membrane protein ectodomain proteolysis GO:0006509 IDA
    Notch signaling pathway GO:0007219 IBA
    Notch receptor processing GO:0007220 IBA
    Notch receptor processing GO:0007220 IDA
    Notch receptor processing GO:0007220 IMP
    Notch receptor processing GO:0007220 NAS
    Notch receptor processing GO:0007220 TAS
    positive regulation of endopeptidase activity GO:0010950 IGI
    positive regulation of endopeptidase activity GO:0010950 IMP
    protein processing GO:0016485 IBA
    protein processing GO:0016485 IDA
    protein processing GO:0016485 IGI
    protein processing GO:0016485 IMP
    membrane protein intracellular domain proteolysis GO:0031293 IDA
    membrane protein intracellular domain proteolysis GO:0031293 IMP
    membrane protein intracellular domain proteolysis GO:0031293 ISS
    amyloid-beta formation GO:0034205 IDA
    amyloid-beta formation GO:0034205 IGI
    amyloid-beta formation GO:0034205 IMP
    amyloid precursor protein metabolic process GO:0042982 IDA
    amyloid precursor protein catabolic process GO:0042987 IDA
    amyloid precursor protein catabolic process GO:0042987 IGI
    amyloid precursor protein catabolic process GO:0042987 IMP
    amyloid precursor protein catabolic process GO:0042987 ISS
    amyloid precursor protein catabolic process GO:0042987 TAS
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    early endosome GO:0005769 IEA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 NAS
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    synaptic vesicle GO:0008021 IEA
    endosome membrane GO:0010008 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    Golgi cisterna membrane GO:0032580 IEA
    presynaptic membrane GO:0042734 IEA
    gamma-secretase complex GO:0070765 IBA
    gamma-secretase complex GO:0070765 IDA
    gamma-secretase complex GO:0070765 IPI
    gamma-secretase complex GO:0070765 ISS
 Experiment description of studies that identified APH1A in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for APH1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 MPP1 4354
Two-hybrid Homo sapiens
3 SMAGP  
Two-hybrid Homo sapiens
4 MFSD6 54842
Two-hybrid Homo sapiens
5 LAT 27040
Two-hybrid Homo sapiens
6 C4orf32  
Affinity Capture-MS Homo sapiens
7 PSEN1 5663
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 NCSTN 23385
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 ASGR1 432
Two-hybrid Homo sapiens
10 SLC39A4 55630
Affinity Capture-MS Homo sapiens
11 PSENEN  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 ATF4  
Two-hybrid Homo sapiens
13 TUSC5  
Two-hybrid Homo sapiens
14 GLP1R  
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
15 TLE1 7088
Two-hybrid Homo sapiens
16 ERLIN2 11160
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
17 TMEM140  
Two-hybrid Homo sapiens
18 BHLHE40  
Two-hybrid Homo sapiens
19 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
20 IGFBP5 3488
Two-hybrid Homo sapiens
21 SNX25 83891
Affinity Capture-MS Homo sapiens
22 FXYD6  
Two-hybrid Homo sapiens
23 HIVEP1 3096
Two-hybrid Homo sapiens
24 SLC38A7 55238
Two-hybrid Homo sapiens
25 PSEN2 5664
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 THPO  
Two-hybrid Homo sapiens
27 SCAND1  
Two-hybrid Homo sapiens
28 NXF1 10482
Affinity Capture-RNA Homo sapiens
29 GPR35  
Two-hybrid Homo sapiens
30 APOD 347
Two-hybrid Homo sapiens
31 SNX19  
Affinity Capture-MS Homo sapiens
32 RBFOX2 23543
Two-hybrid Homo sapiens
33 FIS1 51024
Two-hybrid Homo sapiens
34 MOV10 4343
Affinity Capture-RNA Homo sapiens
35 HES1 3280
Two-hybrid Homo sapiens
36 CREB3  
Two-hybrid Homo sapiens
37 GPATCH8  
Two-hybrid Homo sapiens
38 APH1A 51107
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 KLF11  
Two-hybrid Homo sapiens
40 DDX58 23586
Affinity Capture-RNA Homo sapiens
41 ANKRD46 157567
Two-hybrid Homo sapiens
42 SERP1 27230
Two-hybrid Homo sapiens
43 MRAP2  
Two-hybrid Homo sapiens
44 Psen2  
Affinity Capture-Western Mus musculus
45 DRD2  
Two-hybrid Homo sapiens
46 Psen1  
Affinity Capture-Western Mus musculus
47 TMEM218  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which APH1A is involved
PathwayEvidenceSource
Activated NOTCH1 Transmits Signal to the Nucleus IEA Reactome
Amyloid fiber formation TAS Reactome
Axon guidance TAS Reactome
Cell death signalling via NRAGE, NRIF and NADE TAS Reactome
Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants TAS Reactome
Constitutive Signaling by NOTCH1 PEST Domain Mutants TAS Reactome
Death Receptor Signaling TAS Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
EPH-ephrin mediated repulsion of cells TAS Reactome
EPH-Ephrin signaling TAS Reactome
Metabolism of proteins TAS Reactome
Nervous system development TAS Reactome
Noncanonical activation of NOTCH3 TAS Reactome
NOTCH2 Activation and Transmission of Signal to the Nucleus IEA Reactome
NOTCH3 Activation and Transmission of Signal to the Nucleus TAS Reactome
NOTCH4 Activation and Transmission of Signal to the Nucleus IEA Reactome
NRIF signals cell death from the nucleus TAS Reactome
Nuclear signaling by ERBB4 TAS Reactome
p75 NTR receptor-mediated signalling TAS Reactome
Regulated proteolysis of p75NTR TAS Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by ERBB4 TAS Reactome
Signaling by NOTCH IEA Reactome
Signaling by NOTCH TAS Reactome
Signaling by NOTCH1 IEA Reactome
Signaling by NOTCH1 HD+PEST Domain Mutants in Cancer TAS Reactome
Signaling by NOTCH1 in Cancer TAS Reactome
Signaling by NOTCH1 PEST Domain Mutants in Cancer TAS Reactome
Signaling by NOTCH2 IEA Reactome
Signaling by NOTCH3 TAS Reactome
Signaling by NOTCH4 IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by TGFB family members TAS Reactome
Signaling by TGFBR3 TAS Reactome
TGFBR3 PTM regulation TAS Reactome





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