Gene description for GTPBP2
Gene name GTP binding protein 2
Gene symbol GTPBP2
Other names/aliases -
Species Homo sapiens
 Database cross references - GTPBP2
ExoCarta ExoCarta_54676
Vesiclepedia VP_54676
Entrez Gene 54676
HGNC 4670
MIM 607434
UniProt Q9BX10  
 GTPBP2 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for GTPBP2
Molecular Function
    translation elongation factor activity GO:0003746 IDA
    GTPase activity GO:0003924 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IDA
    identical protein binding GO:0042802 IPI
    alpha-aminoacyl-tRNA binding GO:1904678 IDA
Biological Process
    translational elongation GO:0006414 IBA
Subcellular Localization
    extracellular region GO:0005576 TAS
    platelet alpha granule lumen GO:0031093 TAS
 Experiment description of studies that identified GTPBP2 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GTPBP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRPF3  
Affinity Capture-MS Homo sapiens
2 RNASE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 GPIHBP1  
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 FASN 2194
Negative Genetic Homo sapiens
6 RPL4 6124
Affinity Capture-MS Homo sapiens
7 CUL3 8452
Affinity Capture-MS Homo sapiens
8 TP53 7157
Affinity Capture-MS Homo sapiens
9 GPN3  
Two-hybrid Homo sapiens
10 PRMT5 10419
Two-hybrid Homo sapiens
11 TRIM66  
Affinity Capture-MS Homo sapiens
12 IFI30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 LMNB2 84823
Affinity Capture-MS Homo sapiens
14 RPL10 6134
Affinity Capture-MS Homo sapiens
15 ZNF48  
Affinity Capture-MS Homo sapiens
16 IGFBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 DCP2  
Affinity Capture-MS Homo sapiens
18 PLEKHF2 79666
Two-hybrid Homo sapiens
19 VHL  
Negative Genetic Homo sapiens
20 PLEKHO1 51177
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 IQCF1  
Affinity Capture-MS Homo sapiens
22 SUV39H1  
Two-hybrid Homo sapiens
23 DDX58 23586
Affinity Capture-RNA Homo sapiens
24 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
25 Mus81  
Affinity Capture-MS Mus musculus
26 NXF1 10482
Two-hybrid Homo sapiens
27 MUC20  
Affinity Capture-MS Homo sapiens
28 CHRM3 1131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 POLH  
Affinity Capture-MS Homo sapiens
30 MYCN  
Affinity Capture-MS Homo sapiens
31 CUL9  
Affinity Capture-MS Homo sapiens
32 KDM1A 23028
Two-hybrid Homo sapiens
33 SNRNP70 6625
Two-hybrid Homo sapiens
34 IRF5  
Affinity Capture-MS Homo sapiens
35 PARK2  
Affinity Capture-MS Homo sapiens
36 RAB11A 8766
Affinity Capture-MS Homo sapiens
37 Rrbp1  
Affinity Capture-MS Mus musculus
38 OC90  
Affinity Capture-MS Homo sapiens
39 CLK2 1196
Two-hybrid Homo sapiens
40 STK11 6794
Negative Genetic Homo sapiens
41 LDLR 3949
Negative Genetic Homo sapiens
42 ARRDC3 57561
Affinity Capture-MS Homo sapiens
43 CUL7 9820
Affinity Capture-MS Homo sapiens
44 Junb  
Affinity Capture-MS Mus musculus
45 DNAJC12  
Affinity Capture-MS Homo sapiens
46 HNRNPU 3192
Affinity Capture-MS Homo sapiens
47 Rpl35 66489
Affinity Capture-MS Mus musculus
48 PLD6  
Affinity Capture-MS Homo sapiens
49 GTPBP2 54676
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
50 PMEL 6490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ZNF467  
Affinity Capture-MS Homo sapiens
52 GRAMD1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 RBMS2 5939
Affinity Capture-MS Homo sapiens
54 UBE3D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 IGFL3  
Affinity Capture-MS Homo sapiens
56 PROSER2 254427
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 PRMT6  
Two-hybrid Homo sapiens
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