Experiment description of studies that identified EPS8L1 in sEVs

1 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

7 Experiment ID 193 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ PHB Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

8 Experiment ID 194 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Patients of basement membrane nephropathy Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

9 Experiment ID 195 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Patients of early IgA nephropathy Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

10 Experiment ID 196 MISEV standards ✔ EM Biophysical techniques ✔ Alix|TSG101|HSP70|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22418980     Organism Homo sapiens Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G." Journal name Kidney Int Publication year 2012 Sample Urine Sample name Urine - Normal high density Isolation/purification methods Differential centrifugation Sucrose cushion Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

11 Experiment ID 197 MISEV standards ✔ EM Biophysical techniques ✔ Alix|TSG101|HSP70|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22418980     Organism Homo sapiens Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G." Journal name Kidney Int Publication year 2012 Sample Urine Sample name Urine - Normal low density Isolation/purification methods Differential centrifugation Sucrose cushion Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for EPS8L1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 RIT1 6016 Negative Genetic Homo sapiens 2 PPP2R2B 5521 Affinity Capture-MS Homo sapiens 3 C18orf21   Affinity Capture-MS Homo sapiens 4 ARHGAP27   Affinity Capture-MS Homo sapiens 5 NUDT12   Affinity Capture-MS Homo sapiens 6 AGPAT1 10554 Affinity Capture-MS Homo sapiens 7 WASF2 10163 Affinity Capture-MS Homo sapiens 8 TBC1D22B   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 CDK15   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 DPP8 54878 Affinity Capture-MS Homo sapiens 11 IDE 3416 Affinity Capture-MS Homo sapiens 12 SOS1 6654 Affinity Capture-Western Homo sapiens 13 C1orf35   Affinity Capture-MS Homo sapiens 14 ZC3HC1   Affinity Capture-MS Homo sapiens 15 TERF2IP 54386 Two-hybrid Homo sapiens 16 DHPS 1725 Affinity Capture-MS Homo sapiens 17 NCKAP1 10787 Affinity Capture-MS Homo sapiens 18 CYFIP2 26999 Affinity Capture-MS Homo sapiens 19 SOX2   Affinity Capture-MS Homo sapiens 20 FNDC5   Affinity Capture-MS Homo sapiens 21 BRK1 55845 Affinity Capture-MS Homo sapiens 22 BAIAP2 10458 Affinity Capture-MS Homo sapiens 23 SRRT 51593 Affinity Capture-MS Homo sapiens 24 ABI2 10152 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 25 C1QBP 708 Affinity Capture-MS Homo sapiens 26 CDH1 999 Proximity Label-MS Homo sapiens 27 CYSRT1 375791 Two-hybrid Homo sapiens 28 DIAPH2   Co-fractionation Homo sapiens 29 SSUH2   Affinity Capture-MS Homo sapiens 30 DYRK2 8445 Affinity Capture-MS Homo sapiens 31 STX7 8417 Co-fractionation Homo sapiens 32 CYFIP1 23191 Affinity Capture-MS Homo sapiens 33 OR2A4 79541 Affinity Capture-MS Homo sapiens 34 ABI1 10006 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 35 KBTBD7   Affinity Capture-MS Homo sapiens 36 CCR1   Affinity Capture-MS Homo sapiens 37 STX17 55014 Affinity Capture-MS Homo sapiens 38 ACTG1 71 Affinity Capture-Western Homo sapiens View the network

Pathways in which EPS8L1 is involved

No pathways found