Gene description for UBE2R2
Gene name ubiquitin-conjugating enzyme E2R 2
Gene symbol UBE2R2
Other names/aliases CDC34B
E2-CDC34B
UBC3B
Species Homo sapiens
 Database cross references - UBE2R2
ExoCarta ExoCarta_54926
Vesiclepedia VP_54926
Entrez Gene 54926
HGNC 19907
MIM 612506
UniProt Q712K3  
 UBE2R2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
 Gene ontology annotations for UBE2R2
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin conjugating enzyme activity GO:0061631 IBA
    ubiquitin conjugating enzyme activity GO:0061631 IDA
Biological Process
    protein polyubiquitination GO:0000209 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    protein monoubiquitination GO:0006513 IDA
    protein K48-linked ubiquitination GO:0070936 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified UBE2R2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for UBE2R2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Co-fractionation Homo sapiens
2 YWHAE 7531
Co-fractionation Homo sapiens
3 MEOX1  
Two-hybrid Homo sapiens
4 DZIP3  
Two-hybrid Homo sapiens
5 COPG2 26958
Affinity Capture-MS Homo sapiens
6 CDC34 997
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 BTRC 8945
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
8 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 COPZ1 22818
Affinity Capture-MS Homo sapiens
10 IRF9 10379
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 DTX3L 151636
Two-hybrid Homo sapiens
12 FBXW5  
Two-hybrid Homo sapiens
13 RAD23A 5886
Co-fractionation Homo sapiens
14 C9orf78 51759
Co-fractionation Homo sapiens
15 NUBP2 10101
Co-fractionation Homo sapiens
16 RNF31 55072
Reconstituted Complex Homo sapiens
17 CTNNB1 1499
Biochemical Activity Homo sapiens
18 EZR 7430
Co-fractionation Homo sapiens
19 PAK2 5062
Co-fractionation Homo sapiens
20 UBC 7316
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
21 RNF113B  
Two-hybrid Homo sapiens
22 RBX1 9978
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
23 UBE2R2 54926
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
24 OBFC1 79991
Affinity Capture-MS Homo sapiens
25 COPA 1314
Affinity Capture-MS Homo sapiens
26 BATF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 WDR4 10785
Co-fractionation Homo sapiens
28 UBR7  
Co-fractionation Homo sapiens
29 ARHGDIA 396
Two-hybrid Homo sapiens
30 APPBP2  
Two-hybrid Homo sapiens
31 FASN 2194
Positive Genetic Homo sapiens
32 UBE2D2 7322
Co-fractionation Homo sapiens
33 CTC1  
Affinity Capture-MS Homo sapiens
34 CLIC3 9022
Affinity Capture-MS Homo sapiens
35 DRG2 1819
Co-fractionation Homo sapiens
36 SP110  
Affinity Capture-MS Homo sapiens
37 INF2 64423
Two-hybrid Homo sapiens
38 SDCBP 6386
Two-hybrid Homo sapiens
39 SPANXN4  
Affinity Capture-MS Homo sapiens
40 SMYD3 64754
Affinity Capture-Western Homo sapiens
41 SFN 2810
Co-fractionation Homo sapiens
42 PGAP2  
Affinity Capture-MS Homo sapiens
43 MDM2  
Reconstituted Complex Homo sapiens
44 ARIH2 10425
Two-hybrid Homo sapiens
45 YWHAG 7532
Co-fractionation Homo sapiens
46 PNPLA2  
Two-hybrid Homo sapiens
47 SYT2  
Affinity Capture-MS Homo sapiens
48 ITCH 83737
Reconstituted Complex Homo sapiens
49 GNPDA1 10007
Co-fractionation Homo sapiens
50 UBA1 7317
Reconstituted Complex Homo sapiens
51 TCEANC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 TULP3 7289
Affinity Capture-MS Homo sapiens
53 CBLC  
Two-hybrid Homo sapiens
View the network image/svg+xml



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