ExoCarta: Gene summary

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Gene description for CDC34
Gene name	cell division cycle 34
Gene symbol	CDC34
Other names/aliases	E2-CDC34 UBC3 UBCH3 UBE2R1
Species	Homo sapiens
Database cross references - CDC34
ExoCarta	ExoCarta_997
Vesiclepedia	VP_997
Entrez Gene	997
HGNC	1734
MIM	116948
UniProt	P49427
CDC34 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells	36408942

Gene ontology annotations for CDC34

Molecular Function
	ubiquitin-protein transferase activity	GO:0004842	IDA
	ubiquitin-protein transferase activity	GO:0004842	NAS
	protein binding	GO:0005515	IPI
	ATP binding	GO:0005524	IEA
	ubiquitin conjugating enzyme activity	GO:0061631	IBA
	ubiquitin conjugating enzyme activity	GO:0061631	IDA
Biological Process
	G1/S transition of mitotic cell cycle	GO:0000082	NAS
	protein polyubiquitination	GO:0000209	IBA
	protein polyubiquitination	GO:0000209	IDA
	DNA replication initiation	GO:0006270	NAS
	ubiquitin-dependent protein catabolic process	GO:0006511	IBA
	protein ubiquitination	GO:0016567	IDA
	protein ubiquitination	GO:0016567	NAS
	cellular response to interferon-beta	GO:0035458	IMP
	protein modification process	GO:0036211	NAS
	proteasome-mediated ubiquitin-dependent protein catabolic process	GO:0043161	IDA
	positive regulation of neuron apoptotic process	GO:0043525	IEA
	response to growth factor	GO:0070848	IEA
	protein K48-linked ubiquitination	GO:0070936	IDA
	positive regulation of inclusion body assembly	GO:0090261	IEA
Subcellular Localization
	nucleus	GO:0005634	NAS
	nucleoplasm	GO:0005654	TAS
	cytosol	GO:0005829	IDA
	cytosol	GO:0005829	TAS
	nuclear speck	GO:0016607	IDA

Experiment description of studies that identified CDC34 in sEVs

Experiment ID

488

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD63\|GAPDH\|SDCBP\|LAMP1\|TFRC\|UCHL1\|FLOT2\|LAMP2\|FLOT1\|ICAM1\|RAB5B\|CD151\|RAB35\|TSG101\|RAB5A\|CD82	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for CDC34

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

SIAH1

Two-hybrid

Homo sapiens

ATF5

Affinity Capture-Western

Homo sapiens

FBXO4

26272

Biochemical Activity

Homo sapiens

PPARA

Reconstituted Complex

Homo sapiens

CDC34

997

Biochemical Activity		Homo sapiens
Biochemical Activity		Homo sapiens
Biochemical Activity		Homo sapiens
Biochemical Activity		Homo sapiens
Biochemical Activity		Homo sapiens
Biochemical Activity		Homo sapiens

USP2

Biochemical Activity

Homo sapiens

CUL1

8454

Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
FRET		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens

BTRC

8945

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Reconstituted Complex		Homo sapiens
Biochemical Activity		Homo sapiens
Affinity Capture-MS		Homo sapiens

FBXW11

Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PPP1CB

5500

Affinity Capture-Western

Homo sapiens

TRIM13

Reconstituted Complex

Homo sapiens

CSNK2B

1460

Affinity Capture-Western		Homo sapiens
Biochemical Activity		Homo sapiens
Reconstituted Complex		Homo sapiens

FBXW2

Biochemical Activity

Homo sapiens

CUL2

8453

Reconstituted Complex

Homo sapiens

FAS

355

Proximity Label-MS

Homo sapiens

CUL3

8452

Reconstituted Complex

Homo sapiens

FBXO7

25793

Reconstituted Complex		Homo sapiens
Biochemical Activity		Homo sapiens

RNF167

26001

Reconstituted Complex

Homo sapiens

FBXO32

Reconstituted Complex

Homo sapiens

RAD23A

5886

Co-fractionation

Homo sapiens

RELA

5970

Biochemical Activity

Homo sapiens

SPRYD4

283377

Affinity Capture-MS

Homo sapiens

CORO7

79585

Affinity Capture-Western

Homo sapiens

PPARG

5468

Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens

MARCH1

Reconstituted Complex

Homo sapiens

USP5

8078

Co-fractionation

Homo sapiens

FBXL3

Biochemical Activity

Homo sapiens

NFKBIA

Biochemical Activity

Homo sapiens

MARCH2

Reconstituted Complex

Homo sapiens

UBA1

7317

Biochemical Activity		Homo sapiens
Reconstituted Complex		Homo sapiens
Biochemical Activity		Homo sapiens
Reconstituted Complex		Homo sapiens
Biochemical Activity		Homo sapiens
Biochemical Activity		Homo sapiens

ING4

Reconstituted Complex

Homo sapiens

UBC

7316

Affinity Capture-MS		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Co-crystal Structure		Homo sapiens
Biochemical Activity		Homo sapiens
Biochemical Activity		Homo sapiens
Co-crystal Structure		Homo sapiens
Reconstituted Complex		Homo sapiens
FRET		Homo sapiens
Biochemical Activity		Homo sapiens
Biochemical Activity		Homo sapiens

SKP2

Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Biochemical Activity		Homo sapiens

PIM1

Affinity Capture-Western

Homo sapiens

ZNRF1

Reconstituted Complex

Homo sapiens

RBX1

9978

Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

ITCH

83737

Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens

USP21

Biochemical Activity

Homo sapiens

VHL

Reconstituted Complex

Homo sapiens

CCDC9

Affinity Capture-MS

Homo sapiens

C16orf72

29035

Affinity Capture-MS

Homo sapiens

OBFC1

79991

Affinity Capture-MS

Homo sapiens

CUEDC1

404093

Affinity Capture-MS

Homo sapiens

UBE2R2

54926

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RNF7

Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens

PPP1CC

5501

Affinity Capture-Western

Homo sapiens

ZFAND6

Two-hybrid

Homo sapiens

DIABLO

56616

Biochemical Activity

Homo sapiens

WWP2

11060

Reconstituted Complex		Homo sapiens
Biochemical Activity		Homo sapiens

CTC1

Affinity Capture-MS

Homo sapiens

CCNB1

891

Biochemical Activity

Homo sapiens

RAG1

Reconstituted Complex

Homo sapiens

TRIM28

10155

Reconstituted Complex

Homo sapiens

MDM2

Reconstituted Complex

Homo sapiens

RNF31

55072

Reconstituted Complex

Homo sapiens

SDCBP

6386

Two-hybrid

Homo sapiens

TRIM21

6737

Reconstituted Complex

Homo sapiens

RAD21

5885

Two-hybrid		Homo sapiens
Affinity Capture-Western		Homo sapiens

CDK9

1025

Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens

PTTG1

Biochemical Activity

Homo sapiens

TBL1X

6907

Biochemical Activity

Homo sapiens

FBXW7

Reconstituted Complex

Homo sapiens

STT3B

201595

Affinity Capture-MS

Homo sapiens

UBE2D3

7323

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

TRIM29

23650

Reconstituted Complex

Homo sapiens

CDKN1B

1027

Biochemical Activity		Homo sapiens
Protein-peptide		Homo sapiens

WARS

7453

Co-fractionation

Homo sapiens

SYT2

Affinity Capture-MS

Homo sapiens

HINT1

3094

Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

GNPDA1

10007

Co-fractionation

Homo sapiens

HIST1H2BA

Biochemical Activity

Bos taurus

TCEANC2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NEDD4L

23327

Reconstituted Complex

Homo sapiens

HUWE1

10075

Affinity Capture-MS

Homo sapiens

Rag1

Reconstituted Complex

Mus musculus

HIF1A

3091

Biochemical Activity

Homo sapiens

View the network

Pathways in which CDC34 is involved

Pathway	Evidence	Source
Adaptive Immune System	TAS	Reactome
Antigen processing: Ubiquitination & Proteasome degradation	TAS	Reactome
C-type lectin receptors (CLRs)	TAS	Reactome
Class I MHC mediated antigen processing & presentation	TAS	Reactome
CLEC7A (Dectin-1) signaling	TAS	Reactome
Downstream TCR signaling	TAS	Reactome
Fc epsilon receptor (FCERI) signaling	TAS	Reactome
FCERI mediated NF-kB activation	TAS	Reactome
Immune System	TAS	Reactome
Innate Immune System	TAS	Reactome
Metabolism of proteins	TAS	Reactome
Post-translational protein modification	TAS	Reactome
Protein ubiquitination	TAS	Reactome
Synthesis of active ubiquitin: roles of E1 and E2 enzymes	TAS	Reactome
TCR signaling	TAS	Reactome

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