Gene description for SBF1
Gene name SET binding factor 1
Gene symbol SBF1
Other names/aliases CMT4B3
DENND7A
MTMR5
Species Homo sapiens
 Database cross references - SBF1
ExoCarta ExoCarta_6305
Vesiclepedia VP_6305
Entrez Gene 6305
HGNC 10542
MIM 603560
UniProt O95248  
 SBF1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for SBF1
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein tyrosine/serine/threonine phosphatase activity GO:0008138 TAS
    phosphatase activity GO:0016791 IMP
Biological Process
    protein dephosphorylation GO:0006470 TAS
    spermatid development GO:0007286 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 TAS
    membrane GO:0016020 IBA
    membrane GO:0016020 TAS
    nuclear body GO:0016604 IDA
    neuron projection GO:0043005 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified SBF1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SBF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CIB2  
Affinity Capture-MS Homo sapiens
2 IGFBP3 3486
Affinity Capture-MS Homo sapiens
3 CDH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 HDHD1  
Affinity Capture-MS Homo sapiens
5 APBA3  
Affinity Capture-MS Homo sapiens
6 SNX21  
Affinity Capture-MS Homo sapiens
7 IFT52 51098
Affinity Capture-MS Homo sapiens
8 D2HGDH 728294
Affinity Capture-MS Homo sapiens
9 SNX27 81609
Affinity Capture-MS Homo sapiens
10 SUV39H1  
Co-localization Homo sapiens
Synthetic Rescue Homo sapiens
Affinity Capture-Western Homo sapiens
11 MTMR2 8898
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MAP7 9053
Affinity Capture-MS Homo sapiens
13 STIP1 10963
Affinity Capture-MS Homo sapiens
14 RHOB 388
Proximity Label-MS Homo sapiens
15 SELE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TULP3 7289
Affinity Capture-MS Homo sapiens
17 DNAJC7 7266
Proximity Label-MS Homo sapiens
18 E(z)  
Reconstituted Complex Drosophila melanogaster
19 MECP2 4204
Affinity Capture-MS Homo sapiens
20 RAB6B 51560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PMS1  
Affinity Capture-Western Homo sapiens
22 SLC31A1 1317
Affinity Capture-MS Homo sapiens
23 UQCC2  
Affinity Capture-MS Homo sapiens
24 FBXW5  
Affinity Capture-MS Homo sapiens
25 DHDH  
Affinity Capture-MS Homo sapiens
26 C1orf106  
Affinity Capture-MS Homo sapiens
27 C1orf186  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TNFSF11  
Two-hybrid Homo sapiens
29 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SULT1C4  
Affinity Capture-MS Homo sapiens
31 IFT27 11020
Affinity Capture-MS Homo sapiens
32 CX3CL1  
Affinity Capture-MS Homo sapiens
33 CDKN1A  
Affinity Capture-MS Homo sapiens
34 SBF2 81846
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
35 B4GALT2  
Affinity Capture-MS Homo sapiens
36 PSME1 5720
Affinity Capture-MS Homo sapiens
37 PRPS2 5634
Affinity Capture-MS Homo sapiens
38 TRMT61A 115708
Affinity Capture-MS Homo sapiens
39 DNAJC5 80331
Proximity Label-MS Homo sapiens
40 PIPSL 266971
Affinity Capture-MS Homo sapiens
41 NTRK1 4914
Affinity Capture-MS Homo sapiens
42 PNMA2  
Affinity Capture-MS Homo sapiens
43 ALDH3B1 221
Affinity Capture-MS Homo sapiens
44 MTMR1 8776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 UBXN6 80700
Affinity Capture-MS Homo sapiens
46 KLHL14 57565
Affinity Capture-MS Homo sapiens
47 TNFAIP8L1  
Affinity Capture-MS Homo sapiens
48 PPM1M  
Affinity Capture-MS Homo sapiens
49 Mtmr2  
Affinity Capture-MS Mus musculus
50 KMT2A  
Reconstituted Complex Homo sapiens
51 BTNL9  
Affinity Capture-MS Homo sapiens
52 SIRT7  
Affinity Capture-MS Homo sapiens
53 LAMP1 3916
Proximity Label-MS Homo sapiens
54 WDR55  
Affinity Capture-MS Homo sapiens
55 MEX3A  
Affinity Capture-RNA Homo sapiens
56 CD6 923
Affinity Capture-MS Homo sapiens
57 CTTN 2017
Affinity Capture-MS Homo sapiens
58 LRRC4C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 TFPT  
Affinity Capture-MS Homo sapiens
60 METTL21B  
Affinity Capture-MS Homo sapiens
61 SBF1 6305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 RAB35 11021
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 LZTS2 84445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 AATK  
Two-hybrid Homo sapiens
65 KCNE3  
Affinity Capture-MS Homo sapiens
66 ARF6 382
Proximity Label-MS Homo sapiens
67 CRYBA4  
Affinity Capture-MS Homo sapiens
68 INO80B 83444
Affinity Capture-MS Homo sapiens
69 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
70 WDR5 11091
Affinity Capture-MS Homo sapiens
71 RPAP3 79657
Affinity Capture-MS Homo sapiens
72 DDRGK1 65992
Affinity Capture-MS Homo sapiens
73 SF3B3 23450
Affinity Capture-MS Homo sapiens
74 EFNB2 1948
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 SIRT3  
Affinity Capture-MS Homo sapiens
76 SUPT16H 11198
Affinity Capture-MS Homo sapiens
77 CRYBB3  
Affinity Capture-MS Homo sapiens
78 ZRANB1 54764
Affinity Capture-MS Homo sapiens
79 FTL 2512
Affinity Capture-MS Homo sapiens
80 FBXW11  
Affinity Capture-MS Homo sapiens
81 NSUN5P1  
Affinity Capture-MS Homo sapiens
82 SIRT6  
Affinity Capture-MS Homo sapiens
83 TGFBR2 7048
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 PTGES3L  
Affinity Capture-MS Homo sapiens
85 OPALIN  
Affinity Capture-MS Homo sapiens
86 TRIM52  
Affinity Capture-MS Homo sapiens
87 CTAG1B  
Affinity Capture-MS Homo sapiens
88 HENMT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 UQCC1 55245
Affinity Capture-MS Homo sapiens
91 GFOD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 PRKACA 5566
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 EFNB1 1947
Affinity Capture-MS Homo sapiens
94 HSPB1 3315
Two-hybrid Homo sapiens
95 PDPK1 5170
Two-hybrid Homo sapiens
96 CTTNBP2NL  
Affinity Capture-MS Homo sapiens
97 C11orf52 91894
Proximity Label-MS Homo sapiens
98 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 COL1A2 1278
Affinity Capture-MS Homo sapiens
100 SKAP1 8631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 INF2 64423
Affinity Capture-MS Homo sapiens
102 SSSCA1 10534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 MRPS16  
Affinity Capture-MS Homo sapiens
104 EPHA2 1969
Proximity Label-MS Homo sapiens
105 PTGES3 10728
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 KRAS 3845
Proximity Label-MS Homo sapiens
107 SPRYD7 57213
Affinity Capture-MS Homo sapiens
108 LURAP1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here