Experiment description of studies that identified SEMA3B in sEVs

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for SEMA3B

Protein Interactor ExoCarta ID Identification method PubMed Species 1 LY86   Affinity Capture-MS Homo sapiens 2 P3H1 64175 Affinity Capture-MS Homo sapiens 3 ARSA 410 Affinity Capture-MS Homo sapiens 4 HSPA5 3309 Affinity Capture-MS Homo sapiens 5 LYPD6B 130576 Affinity Capture-MS Homo sapiens 6 Myh3   Affinity Capture-MS Mus musculus 7 FMOD 2331 Affinity Capture-MS Homo sapiens 8 BTNL2   Affinity Capture-MS Homo sapiens 9 NAAA   Affinity Capture-MS Homo sapiens 10 KIF14 9928 Affinity Capture-MS Homo sapiens 11 UQCC1 55245 Affinity Capture-MS Homo sapiens 12 KLK1 3816 Affinity Capture-MS Homo sapiens 13 TAZ   Affinity Capture-MS Homo sapiens 14 TMEM25   Affinity Capture-MS Homo sapiens 15 GLDC 2731 Affinity Capture-MS Homo sapiens 16 GGH 8836 Affinity Capture-MS Homo sapiens 17 BOD1L1 259282 Affinity Capture-MS Homo sapiens 18 TRGV3   Affinity Capture-MS Homo sapiens 19 PDGFRA 5156 Affinity Capture-MS Homo sapiens 20 PRG2   Affinity Capture-MS Homo sapiens 21 MFAP4 4239 Affinity Capture-MS Homo sapiens 22 PI15   Affinity Capture-MS Homo sapiens 23 CBLN4   Affinity Capture-MS Homo sapiens 24 USP11 8237 Affinity Capture-MS Homo sapiens 25 CREBBP   Two-hybrid Homo sapiens 26 SLURP1   Affinity Capture-MS Homo sapiens 27 IL5RA   Affinity Capture-MS Homo sapiens 28 SOX2   Affinity Capture-MS Homo sapiens 29 LYZL6   Affinity Capture-MS Homo sapiens 30 ESR1   Affinity Capture-MS Homo sapiens 31 EPHA7 2045 Affinity Capture-MS Homo sapiens 32 ALPI   Affinity Capture-MS Homo sapiens 33 IGLL5 100423062 Affinity Capture-MS Homo sapiens 34 Lyplal1   Affinity Capture-MS Mus musculus 35 SIRPD   Affinity Capture-MS Homo sapiens 36 SDF2L1 23753 Affinity Capture-MS Homo sapiens 37 IL12RB1   Affinity Capture-MS Homo sapiens 38 BCAN   Affinity Capture-MS Homo sapiens 39 CRISP2   Affinity Capture-MS Homo sapiens 40 C1orf54   Affinity Capture-MS Homo sapiens 41 CFC1   Affinity Capture-MS Homo sapiens 42 ALPP 250 Affinity Capture-MS Homo sapiens 43 DNAJB9 4189 Affinity Capture-MS Homo sapiens 44 CLEC11A 6320 Affinity Capture-MS Homo sapiens 45 C4BPB 725 Affinity Capture-MS Homo sapiens 46 EDN3   Affinity Capture-MS Homo sapiens 47 DNASE1L1 1774 Affinity Capture-MS Homo sapiens 48 TDGF1   Affinity Capture-MS Homo sapiens 49 ISLR 3671 Affinity Capture-MS Homo sapiens 50 PTCH1   Affinity Capture-MS Homo sapiens 51 SLC33A1 9197 Affinity Capture-MS Homo sapiens 52 PAK6 56924 Two-hybrid Homo sapiens View the network

Pathways in which SEMA3B is involved

No pathways found