Gene description for TUBA4B
Gene name tubulin, alpha 4b
Gene symbol TUBA4B
Other names/aliases TUBA4
Species Homo sapiens
 Database cross references - TUBA4B
ExoCarta ExoCarta_80086
Vesiclepedia VP_80086
Entrez Gene 80086
HGNC 18637
 TUBA4B identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Colorectal cancer cells 17956143    
 Gene ontology annotations for TUBA4B
Molecular Function
    hydrolase activity GO:0016787 IEA
    metal ion binding GO:0046872 IEA
 Experiment description of studies that identified TUBA4B in sEVs
1
Experiment ID 76
MISEV standards
EM
Biophysical techniques
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 20
MISEV standards
EM
Biophysical techniques
HSP90|CD63|CD81|LAMP1
Enriched markers
GOLGA2|cytochrome c
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for TUBA4B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
2 TUBB 203068
Co-fractionation Homo sapiens
3 PRKY  
Affinity Capture-MS Homo sapiens
4 GRB2 2885
Affinity Capture-MS Homo sapiens
5 SHC1 6464
Affinity Capture-MS Homo sapiens
6 AP2M1 1173
Affinity Capture-MS Homo sapiens
7 HSPA8 3312
Co-fractionation Homo sapiens
8 CHMP4C 92421
Affinity Capture-MS Homo sapiens
9 BTF3 689
Affinity Capture-MS Homo sapiens
10 CRK 1398
Affinity Capture-MS Homo sapiens
11 CUL5 8065
Affinity Capture-MS Homo sapiens
12 CUL3 8452
Affinity Capture-MS Homo sapiens
13 TUBB4B 10383
Co-fractionation Homo sapiens
14 TFCP2 7024
Affinity Capture-MS Homo sapiens
15 GBA 2629
Affinity Capture-MS Homo sapiens
16 HSPA5 3309
Co-fractionation Homo sapiens
17 CBL 867
Affinity Capture-MS Homo sapiens
18 TUBA4A 7277
Co-fractionation Homo sapiens
19 PPP4R1L  
Affinity Capture-MS Homo sapiens
20 TUBA1C 84790
Co-fractionation Homo sapiens
21 YAP1 10413
Affinity Capture-MS Homo sapiens
22 MCM2 4171
Affinity Capture-MS Homo sapiens
23 CUL4A 8451
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TUBA4B is involved
No pathways found





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