Gene description for CUBN
Gene name cubilin (intrinsic factor-cobalamin receptor)
Gene symbol CUBN
Other names/aliases IFCR
MGA1
gp280
Species Homo sapiens
 Database cross references - CUBN
ExoCarta ExoCarta_8029
Vesiclepedia VP_8029
Entrez Gene 8029
HGNC 2548
MIM 602997
UniProt O60494  
 CUBN identified in sEVs derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 21082674    
Urine 25452312    
 Gene ontology annotations for CUBN
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    cobalamin binding GO:0031419 IEA
    signaling receptor activity GO:0038023 TAS
    cargo receptor activity GO:0038024 EXP
    cargo receptor activity GO:0038024 IDA
    cargo receptor activity GO:0038024 TAS
    protein homodimerization activity GO:0042803 IDA
Biological Process
    tissue homeostasis GO:0001894 NAS
    receptor-mediated endocytosis GO:0006898 NAS
    cholesterol metabolic process GO:0008203 IEA
    cobalamin metabolic process GO:0009235 IDA
    response to bacterium GO:0009617 IEA
    cobalamin transport GO:0015889 IDA
    cobalamin transport GO:0015889 TAS
    lipoprotein transport GO:0042953 IEA
    establishment of localization in cell GO:0051649 IEA
Subcellular Localization
    lysosomal membrane GO:0005765 IEA
    endosome GO:0005768 IEA
    endoplasmic reticulum GO:0005783 IEA
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    clathrin-coated pit GO:0005905 IEA
    membrane GO:0016020 IDA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISS
    apical plasma membrane GO:0016324 NAS
    endocytic vesicle GO:0030139 IDA
    extrinsic component of external side of plasma membrane GO:0031232 NAS
    brush border membrane GO:0031526 ISS
    brush border membrane GO:0031526 NAS
    microvillus membrane GO:0031528 IDA
    lysosomal lumen GO:0043202 TAS
    receptor complex GO:0043235 IPI
    receptor complex GO:0043235 ISS
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified CUBN in sEVs
1
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 102
MISEV standards
✔
IEM
Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21082674    
Organism Homo sapiens
Experiment description "Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS."
Authors "Zhang Y, Li Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Immunoelectron Microscopy
4
Experiment ID 238
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25452312    
Organism Homo sapiens
Experiment description N-linked (N-) glycoproteomics of urimary exosomes
Authors "Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R."
Journal name Mol Cell Proteomics
Publication year 2014
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CUBN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PID1 55022
Affinity Capture-Western Homo sapiens
2 CBL 867
Protein-peptide Homo sapiens
3 GC 2638
Affinity Capture-Western Homo sapiens
4 LRP1 4035
Affinity Capture-Western Homo sapiens
5 Rrp1b 72462
Affinity Capture-MS Mus musculus
6 BRCA1 672
Two-hybrid Homo sapiens
7 RRP1B 23076
Affinity Capture-MS Homo sapiens
8 GIF  
Reconstituted Complex Homo sapiens
9 CUBN 8029
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
10 SCGB1A1  
Reconstituted Complex Homo sapiens
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