Gene ontology annotations for PIP5K1A
Experiment description of studies that identified PIP5K1A in sEVs
1
Experiment ID
489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
363
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
6
Experiment ID
364
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
7
Experiment ID
365
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
8
Experiment ID
488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Homo sapiens
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Mesenchymal stem cells
Sample name
UCMSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
9
Experiment ID
224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
10
Experiment ID
211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
11
Experiment ID
212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
12
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
13
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
Protein-protein interactions for PIP5K1A
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
HNRNPH1
3187
Affinity Capture-RNA
Homo sapiens
2
CENPB
1059
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
3
UBE2H
7328
Affinity Capture-MS
Homo sapiens
4
MARCKS
4082
Proximity Label-MS
Homo sapiens
5
GJA1
2697
Proximity Label-MS
Homo sapiens
6
CSNK1A1
1452
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
7
Calml3
Affinity Capture-MS
Mus musculus
8
RPL10
6134
Affinity Capture-MS
Homo sapiens
9
C9orf78
51759
Affinity Capture-MS
Homo sapiens
10
LAMP2
3920
Proximity Label-MS
Homo sapiens
11
RABGGTB
5876
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
12
APP
351
Reconstituted Complex
Homo sapiens
13
GCH1
Affinity Capture-MS
Homo sapiens
14
HEATR3
55027
Affinity Capture-MS
Homo sapiens
15
ALDH1A1
216
Affinity Capture-MS
Homo sapiens
16
IMPDH2
3615
Affinity Capture-MS
Homo sapiens
17
ZBTB7C
Affinity Capture-MS
Homo sapiens
18
SIRT1
Affinity Capture-MS
Homo sapiens
19
ZSCAN31
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
20
KRAS
3845
Proximity Label-MS
Homo sapiens
21
RAB35
11021
Proximity Label-MS
Homo sapiens
22
ARHGAP22
58504
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
23
PGBD5
Affinity Capture-MS
Homo sapiens
24
LAMTOR1
55004
Proximity Label-MS
Homo sapiens
25
GPR156
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
26
IMPDH1
3614
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
27
CAMK2A
815
Affinity Capture-MS
Homo sapiens
28
ZKSCAN8
Affinity Capture-MS
Homo sapiens
29
CXADR
1525
Proximity Label-MS
Homo sapiens
30
LYN
4067
Proximity Label-MS
Homo sapiens
31
MCAM
4162
Proximity Label-MS
Homo sapiens
32
PRPS2
5634
Affinity Capture-MS
Homo sapiens
33
SRSF6
6431
Affinity Capture-MS
Homo sapiens
34
DNAJC5
80331
Proximity Label-MS
Homo sapiens
35
HIST1H2AM
8336
Affinity Capture-MS
Homo sapiens
36
PFDN2
5202
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
37
PIP5K1C
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
38
MYH9
4627
Affinity Capture-MS
Homo sapiens
39
RAB2A
5862
Proximity Label-MS
Homo sapiens
40
PIP5KL1
Affinity Capture-Western
Homo sapiens
41
RUNDC3A
Affinity Capture-MS
Homo sapiens
42
APBA1
Affinity Capture-MS
Homo sapiens
43
ITGB1BP2
Affinity Capture-Luminescence
Homo sapiens
Affinity Capture-MS
Homo sapiens
44
TGFBR3
7049
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
45
SNAPC1
Affinity Capture-MS
Homo sapiens
46
PELP1
27043
Affinity Capture-MS
Homo sapiens
47
MAGEB2
4113
Affinity Capture-MS
Homo sapiens
48
RAD21
5885
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
49
PIPSL
266971
Affinity Capture-MS
Homo sapiens
50
PER2
Affinity Capture-MS
Homo sapiens
51
RHOU
58480
Reconstituted Complex
Homo sapiens
52
TMED9
54732
Affinity Capture-MS
Homo sapiens
53
PREX1
Affinity Capture-MS
Homo sapiens
54
ABT1
29777
Affinity Capture-MS
Homo sapiens
55
SNAPC4
Affinity Capture-MS
Homo sapiens
56
HTATSF1
27336
Affinity Capture-MS
Homo sapiens
57
LAMP1
3916
Proximity Label-MS
Homo sapiens
58
PIP5K1P1
Affinity Capture-MS
Homo sapiens
59
CD6
923
Affinity Capture-MS
Homo sapiens
60
TRIM41
90933
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
61
ARF6
382
Proximity Label-MS
Homo sapiens
62
SNX27
81609
Affinity Capture-MS
Homo sapiens
63
NXF1
10482
Affinity Capture-RNA
Homo sapiens
64
PIP4K2A
5305
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
65
DGKZ
Affinity Capture-MS
Homo sapiens
66
HECW2
Affinity Capture-MS
Homo sapiens
67
ZC3H18
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
68
ZNF653
Affinity Capture-MS
Homo sapiens
69
RHOB
388
Proximity Label-MS
Homo sapiens
70
Rpl35
66489
Affinity Capture-MS
Mus musculus
71
KBTBD7
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
72
PLXDC2
84898
Affinity Capture-MS
Homo sapiens
73
HUWE1
10075
Affinity Capture-MS
Homo sapiens
74
RPS16
6217
Affinity Capture-MS
Homo sapiens
75
FTL
2512
Affinity Capture-MS
Homo sapiens
76
NRP1
8829
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
77
PARP1
142
Affinity Capture-MS
Homo sapiens
78
CCDC85A
Affinity Capture-MS
Homo sapiens
79
SNAPC3
Affinity Capture-MS
Homo sapiens
80
NRXN3
9369
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
81
FRYL
285527
Affinity Capture-MS
Homo sapiens
82
DNAJA2
10294
Affinity Capture-MS
Homo sapiens
83
KLHDC3
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
84
GFOD1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
85
CAV1
857
Proximity Label-MS
Homo sapiens
86
CCDC97
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
87
C11orf52
91894
Proximity Label-MS
Homo sapiens
88
AHCYL2
23382
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
89
SKAP1
8631
Affinity Capture-MS
Homo sapiens
90
MOV10
4343
Affinity Capture-RNA
Homo sapiens
91
STAG2
10735
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
92
PRKCA
5578
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
93
GPC3
2719
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
94
FLNC
2318
Affinity Capture-MS
Homo sapiens
95
EPHA2
1969
Proximity Label-MS
Homo sapiens
96
KCTD17
79734
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
97
PTGES3
10728
Affinity Capture-MS
Homo sapiens
98
CAMK2D
817
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
99
RC3H1
149041
Affinity Capture-MS
Homo sapiens
100
ZNF467
Affinity Capture-MS
Homo sapiens
101
DCAF5
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
102
ARIH1
25820
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
103
CAPN2
824
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which PIP5K1A is involved