Gene description for MAGT1
Gene name magnesium transporter 1
Gene symbol MAGT1
Other names/aliases IAP
MRX95
OST3B
PRO0756
XMEN
bA217H1.1
Species Homo sapiens
 Database cross references - MAGT1
ExoCarta ExoCarta_84061
Vesiclepedia VP_84061
Entrez Gene 84061
HGNC 28880
MIM 300715
UniProt Q9H0U3  
 MAGT1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MAGT1
Molecular Function
    magnesium ion transmembrane transporter activity GO:0015095 TAS
Biological Process
    protein N-linked glycosylation GO:0006487 IMP
    protein N-linked glycosylation GO:0006487 NAS
    magnesium ion transport GO:0015693 IMP
    protein N-linked glycosylation via asparagine GO:0018279 IBA
    protein N-linked glycosylation via asparagine GO:0018279 IMP
    cognition GO:0050890 IMP
    transmembrane transport GO:0055085 TAS
    magnesium ion transmembrane transport GO:1903830 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 NAS
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    oligosaccharyltransferase complex GO:0008250 IBA
    oligosaccharyltransferase complex GO:0008250 IDA
    oligosaccharyltransferase complex GO:0008250 IPI
    membrane GO:0016020 HDA
    azurophil granule membrane GO:0035577 TAS
 Experiment description of studies that identified MAGT1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MAGT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kif19a  
Affinity Capture-MS Mus musculus
2 SGCA  
Affinity Capture-MS Homo sapiens
3 SLC39A4 55630
Affinity Capture-MS Homo sapiens
4 RPA2 6118
Proximity Label-MS Homo sapiens
5 TUBB8 347688
Affinity Capture-MS Homo sapiens
6 PLD4  
Affinity Capture-MS Homo sapiens
7 TYROBP  
Affinity Capture-MS Homo sapiens
8 VSTM1  
Affinity Capture-MS Homo sapiens
9 DNAJC25 548645
Proximity Label-MS Homo sapiens
10 PTPN1 5770
Proximity Label-MS Homo sapiens
11 SLC22A9  
Affinity Capture-MS Homo sapiens
12 NKIRAS1  
Affinity Capture-MS Homo sapiens
13 B3GNT2 10678
Affinity Capture-MS Homo sapiens
14 CRELD2 79174
Affinity Capture-MS Homo sapiens
15 TMEM231 79583
Affinity Capture-MS Homo sapiens
16 FBXW7  
Affinity Capture-MS Homo sapiens
17 TMED2 10959
Affinity Capture-MS Homo sapiens
18 CLEC2B  
Affinity Capture-MS Homo sapiens
19 SSR1 6745
Co-fractionation Homo sapiens
20 DDOST 1650
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
21 DNAJC16  
Proximity Label-MS Homo sapiens
22 EED  
Affinity Capture-MS Homo sapiens
23 SLC25A46 91137
Proximity Label-MS Homo sapiens
24 TOMM40 10452
Co-fractionation Homo sapiens
25 LDLRAD4  
Affinity Capture-MS Homo sapiens
26 CA14 23632
Affinity Capture-MS Homo sapiens
27 SSR3 6747
Co-fractionation Homo sapiens
28 POGLUT1 56983
Affinity Capture-MS Homo sapiens
29 NCSTN 23385
Affinity Capture-MS Homo sapiens
30 PDPN  
Affinity Capture-MS Homo sapiens
31 LRRC59 55379
Proximity Label-MS Homo sapiens
32 PPIB 5479
Co-fractionation Homo sapiens
33 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
35 LDHD  
Affinity Capture-MS Homo sapiens
36 TUBA1A 7846
Affinity Capture-MS Homo sapiens
37 CHRNB2  
Affinity Capture-MS Homo sapiens
38 Ypel5  
Affinity Capture-MS Mus musculus
39 TUBA1C 84790
Affinity Capture-MS Homo sapiens
40 C11orf1  
Affinity Capture-MS Homo sapiens
41 NPDC1  
Affinity Capture-MS Homo sapiens
42 TCTN2  
Affinity Capture-MS Homo sapiens
43 RPA4  
Proximity Label-MS Homo sapiens
44 RPN1 6184
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 TECR 9524
Co-fractionation Homo sapiens
46 CCDC47 57003
Affinity Capture-MS Homo sapiens
47 VDAC2 7417
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
48 SEC61B 10952
Proximity Label-MS Homo sapiens
49 FOXS1  
Affinity Capture-MS Homo sapiens
50 DNAJB11 51726
Proximity Label-MS Homo sapiens
51 COX4I1 1327
Co-fractionation Homo sapiens
52 SSR4 6748
Co-fractionation Homo sapiens
53 LAT 27040
Affinity Capture-MS Homo sapiens
54 HSPA5 3309
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 DDX6 1656
Affinity Capture-MS Homo sapiens
56 RPN2 6185
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 FOXG1  
Affinity Capture-MS Homo sapiens
58 CANX 821
Affinity Capture-MS Homo sapiens
59 DNAJB12 54788
Proximity Label-MS Homo sapiens
60 BTNL9  
Affinity Capture-MS Homo sapiens
61 CALR3  
Proximity Label-MS Homo sapiens
62 LMNB1 4001
Co-fractionation Homo sapiens
63 CD274 29126
Affinity Capture-MS Homo sapiens
64 DAD1 1603
Affinity Capture-MS Homo sapiens
65 TMEM258  
Affinity Capture-MS Homo sapiens
66 FKBP8 23770
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 FBXO6 26270
Affinity Capture-MS Homo sapiens
68 LRRC32 2615
Affinity Capture-MS Homo sapiens
69 Bach1 12013
Affinity Capture-MS Mus musculus
70 NXF1 10482
Affinity Capture-RNA Homo sapiens
71 CD27  
Affinity Capture-MS Homo sapiens
72 TUBA4A 7277
Affinity Capture-MS Homo sapiens
73 TSPAN5 10098
Affinity Capture-MS Homo sapiens
74 C3orf18  
Affinity Capture-MS Homo sapiens
75 TMEM132A 54972
Affinity Capture-MS Homo sapiens
76 RPA3 6119
Proximity Label-MS Homo sapiens
77 ATP2A1 487
Proximity Label-MS Homo sapiens
78 NCLN 56926
Affinity Capture-MS Homo sapiens
79 DNAJC10 54431
Proximity Label-MS Homo sapiens
80 DNAJC3 5611
Proximity Label-MS Homo sapiens
81 DHFRL1  
Proximity Label-MS Homo sapiens
82 DDRGK1 65992
Affinity Capture-MS Homo sapiens
83 RNF2  
Affinity Capture-MS Homo sapiens
84 VDAC1 7416
Co-fractionation Homo sapiens
85 COX2 4513
Co-fractionation Homo sapiens
86 LMNA 4000
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
87 OST4  
Affinity Capture-MS Homo sapiens
88 PRKCSH 5589
Affinity Capture-MS Homo sapiens
89 TIA1 7072
Affinity Capture-MS Homo sapiens
90 TP53 7157
Affinity Capture-MS Homo sapiens
91 RPL6 6128
Co-fractionation Homo sapiens
92 PDIA4 9601
Proximity Label-MS Homo sapiens
93 CALU 813
Proximity Label-MS Homo sapiens
94 TUBB3 10381
Affinity Capture-MS Homo sapiens
95 NSDHL 50814
Affinity Capture-MS Homo sapiens
96 TOR1AIP1 26092
Affinity Capture-MS Homo sapiens
97 TUBB1 81027
Affinity Capture-MS Homo sapiens
98 ERGIC2 51290
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 PLD3 23646
Affinity Capture-MS Homo sapiens
100 MOV10 4343
Affinity Capture-RNA Homo sapiens
101 NEDD1 121441
Affinity Capture-MS Homo sapiens
102 UGT1A7 54577
Affinity Capture-MS Homo sapiens
103 CD151 977
Affinity Capture-MS Homo sapiens
104 POMK  
Affinity Capture-MS Homo sapiens
105 STT3B 201595
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
106 DNAJC1 64215
Proximity Label-MS Homo sapiens
107 C9orf72  
Affinity Capture-MS Homo sapiens
108 VDAC3 7419
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
109 SEC61A1 29927
Co-fractionation Homo sapiens
110 CLEC4E  
Affinity Capture-MS Homo sapiens
111 TOMM5  
Co-fractionation Homo sapiens
112 EP300 2033
Affinity Capture-MS Homo sapiens
113 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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