Gene description for PUM1
Gene name pumilio RNA-binding family member 1
Gene symbol PUM1
Other names/aliases HSPUM
PUMH
PUMH1
PUML1
Species Homo sapiens
 Database cross references - PUM1
ExoCarta ExoCarta_9698
Vesiclepedia VP_9698
Entrez Gene 9698
HGNC 14957
MIM 607204
UniProt Q14671  
 PUM1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PUM1
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IDA
    mRNA 3'-UTR binding GO:0003730 IBA
    mRNA 3'-UTR binding GO:0003730 IDA
    mRNA 3'-UTR binding GO:0003730 TAS
    protein binding GO:0005515 IPI
    miRNA binding GO:0035198 IDA
Biological Process
    regulation of translation GO:0006417 IEA
    spermatogenesis GO:0007283 ISS
    adult locomotory behavior GO:0008344 ISS
    post-transcriptional regulation of gene expression GO:0010608 IDA
    post-transcriptional regulation of gene expression GO:0010608 IMP
    post-transcriptional gene silencing GO:0016441 ISS
    miRNA processing GO:0035196 IBA
    miRNA processing GO:0035196 IMP
    regulation of mRNA stability GO:0043488 IBA
    regulation of mRNA stability GO:0043488 IDA
    stem cell differentiation GO:0048863 ISS
    regulation of cell cycle GO:0051726 IDA
    regulation of chromosome segregation GO:0051983 IDA
    regulation of miRNA-mediated gene silencing GO:0060964 TAS
    mRNA destabilization GO:0061157 ISS
    3'-UTR-mediated mRNA destabilization GO:0061158 TAS
    positive regulation of RIG-I signaling pathway GO:1900246 IDA
    positive regulation of miRNA-mediated gene silencing GO:2000637 IDA
Subcellular Localization
    P-body GO:0000932 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoplasmic stress granule GO:0010494 IDA
    axon GO:0030424 IEA
 Experiment description of studies that identified PUM1 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PUM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 NUPL2 11097
Affinity Capture-MS Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 HNRNPC 3183
Affinity Capture-MS Homo sapiens
5 MAGEA3  
Affinity Capture-MS Homo sapiens
6 PAICS 10606
Co-fractionation Homo sapiens
7 SPSB4  
Affinity Capture-MS Homo sapiens
8 C9orf78 51759
Affinity Capture-MS Homo sapiens
9 EYA2  
Proximity Label-MS Homo sapiens
10 CAND1 55832
Affinity Capture-MS Homo sapiens
11 LOXL4 84171
Affinity Capture-MS Homo sapiens
12 MSI1  
Affinity Capture-MS Homo sapiens
13 ILF2 3608
Affinity Capture-MS Homo sapiens
14 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
15 VPS26A 9559
Co-fractionation Homo sapiens
16 CAPZB 832
Affinity Capture-MS Homo sapiens
17 RPS20 6224
Proximity Label-MS Homo sapiens
18 GSPT2 23708
Affinity Capture-MS Homo sapiens
19 B3GNT2 10678
Affinity Capture-MS Homo sapiens
20 FBXW7  
Affinity Capture-MS Homo sapiens
21 FBL 2091
Proximity Label-MS Homo sapiens
22 GUSBP5  
Affinity Capture-MS Homo sapiens
23 EFEMP1 2202
Affinity Capture-MS Homo sapiens
24 ITGA4 3676
Affinity Capture-MS Homo sapiens
25 PRTN3 5657
Affinity Capture-MS Homo sapiens
26 FAM120A 23196
Affinity Capture-MS Homo sapiens
27 LARP4B 23185
Affinity Capture-MS Homo sapiens
28 HSPA8 3312
Affinity Capture-MS Homo sapiens
29 LIN28A  
Affinity Capture-MS Homo sapiens
30 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CPEB1  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 HSPBP1 23640
Affinity Capture-MS Homo sapiens
33 KRT8 3856
Proximity Label-MS Homo sapiens
34 CLOCK  
Affinity Capture-MS Homo sapiens
35 SEPHS1 22929
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
36 CDKN1A  
Affinity Capture-MS Homo sapiens
37 SPSB2  
Affinity Capture-MS Homo sapiens
38 CLNS1A 1207
Co-fractionation Homo sapiens
39 PURG  
Affinity Capture-MS Homo sapiens
40 NTRK1 4914
Affinity Capture-MS Homo sapiens
41 TRIM11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 KDF1  
Affinity Capture-MS Homo sapiens
43 ELAVL2 1993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 ATXN1 6310
Two-hybrid Homo sapiens
45 CYB5B 80777
Affinity Capture-MS Homo sapiens
46 XPO1 7514
Affinity Capture-MS Homo sapiens
47 BAG2 9532
Affinity Capture-MS Homo sapiens
48 RBM47 54502
Affinity Capture-MS Homo sapiens
49 SIRT7  
Affinity Capture-MS Homo sapiens
50 FOXG1  
Affinity Capture-MS Homo sapiens
51 EGFR 1956
Affinity Capture-MS Homo sapiens
52 FBXO6 26270
Affinity Capture-MS Homo sapiens
53 CELF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 AURKB 9212
Affinity Capture-MS Homo sapiens
55 SMAD1 4086
Two-hybrid Homo sapiens
56 PRR3  
Affinity Capture-MS Homo sapiens
57 SAV1  
Proximity Label-MS Homo sapiens
58 SEC16A 9919
Affinity Capture-MS Homo sapiens
59 PABPC5 140886
Affinity Capture-MS Homo sapiens
60 CLASRP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 HSPA1A 3303
Affinity Capture-MS Homo sapiens
62 DSTN 11034
Co-fractionation Homo sapiens
63 MATR3 9782
Affinity Capture-MS Homo sapiens
64 OTULIN 90268
Affinity Capture-MS Homo sapiens
65 BMI1  
Affinity Capture-MS Homo sapiens
66 FOXQ1  
Affinity Capture-MS Homo sapiens
67 AKAP1 8165
Proximity Label-MS Homo sapiens
68 RBMS2 5939
Affinity Capture-MS Homo sapiens
69 CCDC8  
Affinity Capture-MS Homo sapiens
70 FOXI2  
Affinity Capture-MS Homo sapiens
71 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
72 FBXW11  
Affinity Capture-MS Homo sapiens
73 APOBEC3D  
Affinity Capture-MS Homo sapiens
74 LITAF 9516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 HSPB1 3315
Co-fractionation Homo sapiens
76 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
77 NUP35 129401
Proximity Label-MS Homo sapiens
78 PRR12 57479
Two-hybrid Homo sapiens
79 LATS1  
Proximity Label-MS Homo sapiens
80 UFL1 23376
Affinity Capture-MS Homo sapiens
81 RBFOX2 23543
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 E2F4  
Affinity Capture-MS Homo sapiens
83 LAGE3  
Co-fractionation Homo sapiens
84 ANXA5 308
Co-fractionation Homo sapiens
85 C9orf72  
Affinity Capture-MS Homo sapiens
86 RC3H1 149041
Affinity Capture-MS Homo sapiens
87 EP300 2033
Affinity Capture-MS Homo sapiens
88 EIF5 1983
Two-hybrid Homo sapiens
View the network image/svg+xml



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