Gene description for USP6NL
Gene name USP6 N-terminal like
Gene symbol USP6NL
Other names/aliases RNTRE
TRE2NL
USP6NL-IT1
Species Homo sapiens
 Database cross references - USP6NL
ExoCarta ExoCarta_9712
Vesiclepedia VP_9712
Entrez Gene 9712
HGNC 16858
MIM 605405
UniProt Q92738  
 USP6NL identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
 Gene ontology annotations for USP6NL
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IDA
    small GTPase binding GO:0031267 IPI
Biological Process
    Golgi organization GO:0007030 IMP
    virion assembly GO:0019068 IMP
    retrograde transport, plasma membrane to Golgi GO:0035526 IMP
    positive regulation of GTPase activity GO:0043547 IDA
    plasma membrane to endosome transport GO:0048227 IBA
    plasma membrane to endosome transport GO:0048227 IMP
    regulation of cilium assembly GO:1902017 IMP
    regulation of Golgi organization GO:1903358 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    cytoplasmic vesicle GO:0031410 IBA
    cytoplasmic vesicle GO:0031410 IDA
    trans-Golgi network membrane GO:0032588 TAS
 Experiment description of studies that identified USP6NL in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for USP6NL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRRC15 131578
Affinity Capture-MS Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 RNF43  
Proximity Label-MS Homo sapiens
4 GJD3  
Proximity Label-MS Homo sapiens
5 UBE2S  
Affinity Capture-MS Homo sapiens
6 OCLN 100506658
Proximity Label-MS Homo sapiens
7 RAB35 11021
Proximity Label-MS Homo sapiens
8 CCNA2 890
Affinity Capture-MS Homo sapiens
9 LAMTOR1 55004
Proximity Label-MS Homo sapiens
10 MCAM 4162
Proximity Label-MS Homo sapiens
11 GRB2 2885
Reconstituted Complex Homo sapiens
12 KIF14 9928
Affinity Capture-MS Homo sapiens
13 MARCKS 4082
Proximity Label-MS Homo sapiens
14 ATG9A 79065
Proximity Label-MS Homo sapiens
15 GJA1 2697
Proximity Label-MS Homo sapiens
16 ECT2 1894
Affinity Capture-MS Homo sapiens
17 PARD3 56288
Proximity Label-MS Homo sapiens
18 EPB41L4A 64097
Proximity Label-MS Homo sapiens
19 CTNNB1 1499
Proximity Label-MS Homo sapiens
20 ANAPC15  
Affinity Capture-MS Homo sapiens
21 LCK 3932
Proximity Label-MS Homo sapiens
22 VSIG8  
Affinity Capture-MS Homo sapiens
23 FAM168A  
Affinity Capture-MS Homo sapiens
24 WWOX 51741
Affinity Capture-MS Homo sapiens
25 ARF6 382
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SHMT2 6472
Affinity Capture-RNA Homo sapiens
27 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
28 C11orf52 91894
Proximity Label-MS Homo sapiens
29 Map3k12  
Affinity Capture-MS Mus musculus
30 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
31 LYN 4067
Proximity Label-MS Homo sapiens
32 NXF1 10482
Affinity Capture-RNA Homo sapiens
33 PLCD1 5333
Affinity Capture-MS Homo sapiens
34 WWP2 11060
Affinity Capture-MS Homo sapiens
35 Ppp2ca 19052
Affinity Capture-MS Mus musculus
36 DNAJC5 80331
Proximity Label-MS Homo sapiens
37 Cenpf  
Affinity Capture-MS Mus musculus
38 CDH1 999
Proximity Label-MS Homo sapiens
39 MLLT4 4301
Proximity Label-MS Homo sapiens
40 Dctn3  
Affinity Capture-MS Mus musculus
41 DIRAS3  
Proximity Label-MS Homo sapiens
42 ACTN4 81
Affinity Capture-Western Homo sapiens
43 WWC1  
Affinity Capture-MS Homo sapiens
44 DSG4 147409
Affinity Capture-MS Homo sapiens
45 CXADR 1525
Proximity Label-MS Homo sapiens
46 DTX2 113878
Proximity Label-MS Homo sapiens
47 LAMP1 3916
Proximity Label-MS Homo sapiens
48 DUSP14 11072
Affinity Capture-MS Homo sapiens
49 EPHA2 1969
Proximity Label-MS Homo sapiens
50 Cetn2  
Affinity Capture-MS Mus musculus
51 SNX27 81609
Affinity Capture-MS Homo sapiens
52 KRAS 3845
Proximity Label-MS Homo sapiens
53 RHOB 388
Proximity Label-MS Homo sapiens
54 CAV1 857
Proximity Label-MS Homo sapiens
55 FLOT1 10211
Proximity Label-MS Homo sapiens
56 COMT 1312
Affinity Capture-MS Homo sapiens
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