ExoCarta: Gene summary

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Gene description for ARHGEF17
Gene name	Rho guanine nucleotide exchange factor (GEF) 17
Gene symbol	ARHGEF17
Other names/aliases	P164RHOGEF TEM4 p164-RhoGEF
Species	Homo sapiens
Database cross references - ARHGEF17
ExoCarta	ExoCarta_9828
Vesiclepedia	VP_9828
Entrez Gene	9828
HGNC	21726
UniProt	Q96PE2
ARHGEF17 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Liver cancer cells	25265333
Liver cancer cells	25265333
Mesenchymal stem cells	36408942

Gene ontology annotations for ARHGEF17

Molecular Function
	guanyl-nucleotide exchange factor activity	GO:0005085	IBA
	guanyl-nucleotide exchange factor activity	GO:0005085	IDA
	guanyl-nucleotide exchange factor activity	GO:0005085	TAS
	protein binding	GO:0005515	IPI
Biological Process
	actin cytoskeleton organization	GO:0030036	IBA
	actin cytoskeleton organization	GO:0030036	IDA
	regulation of small GTPase mediated signal transduction	GO:0051056	TAS
Subcellular Localization
	cytoplasm	GO:0005737	IBA
	cytosol	GO:0005829	TAS

Experiment description of studies that identified ARHGEF17 in sEVs

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

266

MISEV standards
✘	Biophysical techniques
✔ TSG101\|Alix	Enriched markers
✔ CANX\|HSP90B1	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

25265333

Organism

Homo sapiens

Experiment description

Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS

Authors

"Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"

Journal name

J Proteome Res

Publication year

2014

Sample

Liver cancer cells

Sample name

Huh-7 - Wild type

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

268

MISEV standards
✘	Biophysical techniques
✔ TSG101\|Alix	Enriched markers
✔ CANX\|HSP90B1	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

25265333

Organism

Homo sapiens

Experiment description

Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS

Authors

"Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"

Journal name

J Proteome Res

Publication year

2014

Sample

Liver cancer cells

Sample name

Huh-7 - Transfected with HBx-null HBV replicon plasmids

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

488

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD63\|GAPDH\|SDCBP\|LAMP1\|TFRC\|UCHL1\|FLOT2\|LAMP2\|FLOT1\|ICAM1\|RAB5B\|CD151\|RAB35\|TSG101\|RAB5A\|CD82	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for ARHGEF17

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

YWHAH

7533

Affinity Capture-MS

Homo sapiens

GSN

2934

Affinity Capture-MS

Homo sapiens

SFN

2810

Affinity Capture-MS

Homo sapiens

ANLN

54443

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPL10L

140801

Affinity Capture-MS

Homo sapiens

KIF14

9928

Affinity Capture-MS

Homo sapiens

PRDX4

10549

Affinity Capture-MS

Homo sapiens

Tpm1

22003

Affinity Capture-MS

Mus musculus

DOCK4

9732

Affinity Capture-MS

Homo sapiens

TP53

7157

Affinity Capture-MS

Homo sapiens

LBR

3930

Affinity Capture-MS

Homo sapiens

YWHAB

7529

Affinity Capture-MS

Homo sapiens

ECT2

1894

Affinity Capture-MS

Homo sapiens

TARDBP

23435

Affinity Capture-MS

Homo sapiens

E2F4

Affinity Capture-MS

Homo sapiens

PRDX2

7001

Affinity Capture-MS

Homo sapiens

MEN1

4221

Affinity Capture-MS

Homo sapiens

MYO18A

399687

Affinity Capture-MS

Homo sapiens

EFNA1

Affinity Capture-MS

Homo sapiens

CAPZA2

830

Affinity Capture-MS

Homo sapiens

CALM1

801

Affinity Capture-MS

Homo sapiens

OTX1

Two-hybrid

Homo sapiens

Myh10

77579

Affinity Capture-MS

Mus musculus

NXF1

10482

Affinity Capture-RNA

Homo sapiens

EFNA3

Affinity Capture-MS

Homo sapiens

SYNPO

11346

Affinity Capture-MS

Homo sapiens

CHMP4B

128866

Affinity Capture-MS

Homo sapiens

RASL11A

Affinity Capture-MS

Homo sapiens

PRC1

9055

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MYH9

4627

Affinity Capture-MS

Homo sapiens

DYRK1A

1859

Affinity Capture-MS

Homo sapiens

TMOD2

29767

Affinity Capture-MS

Homo sapiens

RASL12

51285

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

WDR83

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

C14orf166

51637

Affinity Capture-MS

Homo sapiens

Vamp2

Affinity Capture-MS

Mus musculus

IQGAP1

8826

Affinity Capture-MS

Homo sapiens

Coro1c

23790

Affinity Capture-MS

Mus musculus

YWHAG

7532

Affinity Capture-MS

Homo sapiens

RHOA

387

FRET

Homo sapiens

Kif2a

16563

Affinity Capture-MS

Mus musculus

RERG

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

View the network

Pathways in which ARHGEF17 is involved

Pathway	Evidence	Source
Cell death signalling via NRAGE, NRIF and NADE	TAS	Reactome
Death Receptor Signaling	TAS	Reactome
G alpha (12/13) signalling events	TAS	Reactome
GPCR downstream signalling	TAS	Reactome
NRAGE signals death through JNK	TAS	Reactome
p75 NTR receptor-mediated signalling	TAS	Reactome
RHO GTPase cycle	TAS	Reactome
RHOA GTPase cycle	TAS	Reactome
RHOB GTPase cycle	TAS	Reactome
RHOC GTPase cycle	TAS	Reactome
Signal Transduction	TAS	Reactome
Signaling by GPCR	TAS	Reactome
Signaling by Rho GTPases	TAS	Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3	TAS	Reactome

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